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The map label for this gene is lasT [C]

Identifier: 124022607

GI number: 124022607

Start: 814391

End: 815140

Strand: Reverse

Name: lasT [C]

Synonym: P9303_08981

Alternate gene names: 124022607

Gene position: 815140-814391 (Counterclockwise)

Preceding gene: 124022609

Following gene: 124022606

Centisome position: 30.39

GC content: 56.27

Gene sequence:

>750_bases
TTGAACAAGGACATCCGCTCAATCCAAACGACTGTTGTTCTGGTTGAGCCAATTGGTCCACTGAATCTGGGCAGTGTGGC
CAGACTCTGCGCCAACTTCGGAGTACAAGAACTACGTCTGGTCTCGCCCCGCTGCGACCCTGCTGATGCCGAGGCACGAC
GCATGGCTGTCCATGGCGCAGCAATGCTTGAGCAGGCAAAACAATTCTCATGCTTGCTCGATGCCGTAGCTGACTGTCAC
AGAGTTGTAGCCAGCTGCGGCCGATTGGATCATGGCAACATCCCCCTGCAACCCCCCGAACAAGCAGTGCCATGGTTACT
AGAGAGCATGGGTGCAGCACCTGTGGCACTGGTATTTGGTCGTGAAGACAGGGGCCTAACCAACGAGGAACTTCAACTCG
CCCAACGTGTAATCACTCTGCACAGCTCTTATGGATATCCATCCCTCAACCTCTCGCATGCAGTAGCGATCGTGCTGCAT
GAGCTACAACGTTGCCAAGGGCAAGGGACGCTCAGGGGAGGGCAATCGGCATGGCCCGACCCAGCATCACCTCGTCAACT
CGACGCATGCTTAACAGACGCACAGGATCTCCTGTTAGAGGTGGGATTTCTTTTGAGCCATACGGCCCAAGCCCGCATGG
CCAAAGTCCGTGGTTTACTCCAACGCGCTGCCGTCCGTCCCGAGGAAGTAGCCCTGCTGAGAGGGATGGTGAGGCAACTG
CGCTGGGCTATCCATTCTCGTCACTCTTAA

Upstream 100 bases:

>100_bases
CGTTCCATAACGCCAAACAAACCCCAGTTCATCCAACTTCATCTAACCTCCAACCCCAGCTTTTCCTAGTGAGAGCCTTA
ACCTTTATACTTTCCCTGAA

Downstream 100 bases:

>100_bases
CCTCAGCCGAACTCACTGATCTTCACTTCCTTGTCAAGCAACCGCCCTTACCGCAACCGCTCAAACGGGGCTGGTCCCCT
ACGGCTTTTGTTGCGTTTGA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNKDIRSIQTTVVLVEPIGPLNLGSVARLCANFGVQELRLVSPRCDPADAEARRMAVHGAAMLEQAKQFSCLLDAVADCH
RVVASCGRLDHGNIPLQPPEQAVPWLLESMGAAPVALVFGREDRGLTNEELQLAQRVITLHSSYGYPSLNLSHAVAIVLH
ELQRCQGQGTLRGGQSAWPDPASPRQLDACLTDAQDLLLEVGFLLSHTAQARMAKVRGLLQRAAVRPEEVALLRGMVRQL
RWAIHSRHS

Sequences:

>Translated_249_residues
MNKDIRSIQTTVVLVEPIGPLNLGSVARLCANFGVQELRLVSPRCDPADAEARRMAVHGAAMLEQAKQFSCLLDAVADCH
RVVASCGRLDHGNIPLQPPEQAVPWLLESMGAAPVALVFGREDRGLTNEELQLAQRVITLHSSYGYPSLNLSHAVAIVLH
ELQRCQGQGTLRGGQSAWPDPASPRQLDACLTDAQDLLLEVGFLLSHTAQARMAKVRGLLQRAAVRPEEVALLRGMVRQL
RWAIHSRHS
>Mature_249_residues
MNKDIRSIQTTVVLVEPIGPLNLGSVARLCANFGVQELRLVSPRCDPADAEARRMAVHGAAMLEQAKQFSCLLDAVADCH
RVVASCGRLDHGNIPLQPPEQAVPWLLESMGAAPVALVFGREDRGLTNEELQLAQRVITLHSSYGYPSLNLSHAVAIVLH
ELQRCQGQGTLRGGQSAWPDPASPRQLDACLTDAQDLLLEVGFLLSHTAQARMAKVRGLLQRAAVRPEEVALLRGMVRQL
RWAIHSRHS

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=228, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790865, Length=159, Percent_Identity=35.2201257861635, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27174; Mature: 27174

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDIRSIQTTVVLVEPIGPLNLGSVARLCANFGVQELRLVSPRCDPADAEARRMAVHGA
CCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHH
AMLEQAKQFSCLLDAVADCHRVVASCGRLDHGNIPLQPPEQAVPWLLESMGAAPVALVFG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
REDRGLTNEELQLAQRVITLHSSYGYPSLNLSHAVAIVLHELQRCQGQGTLRGGQSAWPD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PASPRQLDACLTDAQDLLLEVGFLLSHTAQARMAKVRGLLQRAAVRPEEVALLRGMVRQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RWAIHSRHS
HHHHHHCCC
>Mature Secondary Structure
MNKDIRSIQTTVVLVEPIGPLNLGSVARLCANFGVQELRLVSPRCDPADAEARRMAVHGA
CCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHH
AMLEQAKQFSCLLDAVADCHRVVASCGRLDHGNIPLQPPEQAVPWLLESMGAAPVALVFG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
REDRGLTNEELQLAQRVITLHSSYGYPSLNLSHAVAIVLHELQRCQGQGTLRGGQSAWPD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PASPRQLDACLTDAQDLLLEVGFLLSHTAQARMAKVRGLLQRAAVRPEEVALLRGMVRQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RWAIHSRHS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]