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The map label for this gene is gloB

Identifier: 124022531

GI number: 124022531

Start: 747195

End: 747977

Strand: Reverse

Name: gloB

Synonym: P9303_08221

Alternate gene names: 124022531

Gene position: 747977-747195 (Counterclockwise)

Preceding gene: 124022535

Following gene: 124022529

Centisome position: 27.88

GC content: 52.62

Gene sequence:

>783_bases
ATGGAGGCAACCTCAACAGCTGCGACTAGCGATAAACAACGTTCATCGATCCATGCCCTTCCTGTTCTTCAGGACAACAT
CATCTGGATCTGGATTAAAGGCGACCAAGCTGTCGTTGTGGATCCAGCAATCGCAGAACCAGTCAAGACATGGCTGCAAA
CTCGAAAGCTCTCACTCGCAGCAGTACTTCAGACCCATCACCATGCTGATCACATTGGTGGCACCCTTGAGCTGCTTAGG
GACTGGCCTAACGCAGCCGTGGTAGCAGCCGCTGACGACCGTGATCGCATTCCGTTCCAGACGATCTCCGTTCGTGATAG
AGACAAGATTTCTCTTCTAAACAGCAGTGTTGAGGTGCTCGCCGTTGCGGGTCATACCAGAGCACACATTGCTTATTACC
TGCCCACAAACAAGGAAGATCGTGAAGATCCAGCAGTGTTCTGTGGTGACACCCTTTTCGGAGCTGGATGTGGCCGACTG
TTTGAAGGAACTGCCGAAGACATGTTCAAGGCTCTTCAACGTCTCTGTTGCCTGCCAGCGAAGACCCGTGTGTATTGCGC
TCACGAATACACCGAAGCAAATCTGCGCTGGGCCTCCGCCCTGCATCCAGAAGATATTGCTATCAGCGAACGACTGGTAG
ACGTTTCAAGTCGCCGCCAGAGGGGTGCCCTGAGCCTGCCTAGCAGCATTTCAGAAGAACAACGTACCAACCTCTTCGTG
CGTGCTCAGAACAGCAAAGAGCTAGCGGAACTTCGCCAACACAAGGATCAATGGCGAAACTAA

Upstream 100 bases:

>100_bases
AACCCAGCAGATTGAAAACGAGCAACCGAATCATTCAGCAAAGCTCCTTTTGCAAGCGCGACAGTGATCATGGAGCGATG
CGTCGACCTGGACTTTAACG

Downstream 100 bases:

>100_bases
GCACAAATATCTGACGCAGCAAGATCAAAATGGCCTGCCCTGGCAAAACTGCTCAGCAACAAGGCAGTCCTGTTCCCTTG
GAACAGGACCAACAGCCAGT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGDQAVVVDPAIAEPVKTWLQTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAADDRDRIPFQTISVRDRDKISLLNSSVEVLAVAGHTRAHIAYYLPTNKEDREDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLCCLPAKTRVYCAHEYTEANLRWASALHPEDIAISERLVDVSSRRQRGALSLPSSISEEQRTNLFV
RAQNSKELAELRQHKDQWRN

Sequences:

>Translated_260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGDQAVVVDPAIAEPVKTWLQTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAADDRDRIPFQTISVRDRDKISLLNSSVEVLAVAGHTRAHIAYYLPTNKEDREDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLCCLPAKTRVYCAHEYTEANLRWASALHPEDIAISERLVDVSSRRQRGALSLPSSISEEQRTNLFV
RAQNSKELAELRQHKDQWRN
>Mature_260_residues
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGDQAVVVDPAIAEPVKTWLQTRKLSLAAVLQTHHHADHIGGTLELLR
DWPNAAVVAAADDRDRIPFQTISVRDRDKISLLNSSVEVLAVAGHTRAHIAYYLPTNKEDREDPAVFCGDTLFGAGCGRL
FEGTAEDMFKALQRLCCLPAKTRVYCAHEYTEANLRWASALHPEDIAISERLVDVSSRRQRGALSLPSSISEEQRTNLFV
RAQNSKELAELRQHKDQWRN

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=266, Percent_Identity=36.0902255639098, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=36.4341085271318, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI116642887, Length=225, Percent_Identity=34.6666666666667, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=35.0877192982456, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI21703352, Length=225, Percent_Identity=34.6666666666667, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI46361987, Length=199, Percent_Identity=36.1809045226131, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786406, Length=232, Percent_Identity=36.2068965517241, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17536925, Length=252, Percent_Identity=33.7301587301587, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=230, Percent_Identity=32.6086956521739, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=181, Percent_Identity=33.1491712707182, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=36.6795366795367, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=36.6795366795367, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=36.6795366795367, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221330176, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_PROM3 (A2C7W3)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016838.1
- ProteinModelPortal:   A2C7W3
- SMR:   A2C7W3
- STRING:   A2C7W3
- GeneID:   4777903
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_08221
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK647185
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 29082; Mature: 29082

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGDQAVVVDPAIAEPVKTWLQTRKLSLA
CCCCCCCCCCCHHHCCEEECEEEECCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHH
AVLQTHHHADHIGGTLELLRDWPNAAVVAAADDRDRIPFQTISVRDRDKISLLNSSVEVL
HHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHCCCEEEE
AVAGHTRAHIAYYLPTNKEDREDPAVFCGDTLFGAGCGRLFEGTAEDMFKALQRLCCLPA
EEECCCEEEEEEECCCCCCCCCCCCEEECCCHHCCCCCHHHCCCHHHHHHHHHHHHCCCC
KTRVYCAHEYTEANLRWASALHPEDIAISERLVDVSSRRQRGALSLPSSISEEQRTNLFV
CCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEE
RAQNSKELAELRQHKDQWRN
EECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEATSTAATSDKQRSSIHALPVLQDNIIWIWIKGDQAVVVDPAIAEPVKTWLQTRKLSLA
CCCCCCCCCCCHHHCCEEECEEEECCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHHHH
AVLQTHHHADHIGGTLELLRDWPNAAVVAAADDRDRIPFQTISVRDRDKISLLNSSVEVL
HHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHCCCEEEE
AVAGHTRAHIAYYLPTNKEDREDPAVFCGDTLFGAGCGRLFEGTAEDMFKALQRLCCLPA
EEECCCEEEEEEECCCCCCCCCCCCEEECCCHHCCCCCHHHCCCHHHHHHHHHHHHCCCC
KTRVYCAHEYTEANLRWASALHPEDIAISERLVDVSSRRQRGALSLPSSISEEQRTNLFV
CCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEE
RAQNSKELAELRQHKDQWRN
EECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA