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The map label for this gene is truB [H]

Identifier: 124022249

GI number: 124022249

Start: 521937

End: 522908

Strand: Reverse

Name: truB [H]

Synonym: P9303_05391

Alternate gene names: 124022249

Gene position: 522908-521937 (Counterclockwise)

Preceding gene: 124022250

Following gene: 124022248

Centisome position: 19.49

GC content: 57.2

Gene sequence:

>972_bases
GTGCCCTTCGGCTTTGTGGTGATCGACAAGCCTGCTGGGCTCACCTCCCATGACTGTGTGAGCCGAATGCGGCGCGTGTT
TGGCATCAAGCGCGTGGGCCACGGCGGCACGCTTGATCCTGCCGTAACAGGAGTCTTGCCCATCGCCCTAGGGCATGCCA
CCAGGCTGCTGCCCTATCTACCCGGCGCCAAGAGCTATCGCGGCAGCATCCAACTGGGCCAACGCACCAGTAGCGACGAT
CAACAAGGGGATCTGATCTCCAAGCAAGCCTGGCCCGAATTGAACACAGCAGAGATCGAGGCCTATCTCGAGCCGTTTCG
CGGCAGGATTCAGCAACGGCCACCGCAAGTTTCAGCCGTGCACGTGCAAGGGGAACGCGCCTATGCACGTGCACGCAGGG
GAGAAACGATGGAGATTCCAGCGCGAACGATCACGATTGATTGCTTGCAGCTACTCAGTTGGAACCAACAACTGGGCCAA
ATTGACTTCAACGTGCATTGCTCATCAGGCACATACATTCGCTCTCTCGCACGAGATCTAGGCGAATTAATTGGCTGCGG
CGCTTGTCTGGGGTGGTTAAAGCGAACCCAGGCACTGGGGTTTCACGAGCAACAAGCCGTACCACTACCCGATCGAGACA
ATCCAGTCCTAACGACTCCGCCTGCTGTGTTACCGCCACTCACGGCCCTTGCACACCTACCAAGGCTGCAACTCAACGAA
GAGGAACAGGAGAGCTGGAGCTGCGGGCGCCGCATTACCGCCCATCAAGATCAATGCCAACCAGCACCCAAACCTCTGGC
CTCTGATCAGCAAGAATCTGATCCAAGCCAAACTGCTCCAAACCAAACTGATCCAAGCGCAAACAAGCCCATGCTCGTGG
TGATCGACTGCAGAGGCGAAGTTGCCGGAATGGCCTACTGGGAAGATAACGCAACAGTGAAGCCAAAAGTTGTGTTCAAC
GCACGGGGATGA

Upstream 100 bases:

>100_bases
CAAGCGCATCAGTGTGGTTGCCTCCAGCTGAGCAATCTCAGCCTTTGCAGACAATGCGGGCAGCAGCAGTCACAAACTGA
ACGCTGCTGATCAGGTGAGT

Downstream 100 bases:

>100_bases
TCGCAACACTGTCGTTCAACGCGAACGCCCCTAGCGTTGATGGTCAAGCAACAAGCTAATCAGCCTCTGCCATGGCCGGC
CATAGCAAATGGTCTCAAAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPFGFVVIDKPAGLTSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSSDD
QQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAYARARRGETMEIPARTITIDCLQLLSWNQQLGQ
IDFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPVLTTPPAVLPPLTALAHLPRLQLNE
EEQESWSCGRRITAHQDQCQPAPKPLASDQQESDPSQTAPNQTDPSANKPMLVVIDCRGEVAGMAYWEDNATVKPKVVFN
ARG

Sequences:

>Translated_323_residues
MPFGFVVIDKPAGLTSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSSDD
QQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAYARARRGETMEIPARTITIDCLQLLSWNQQLGQ
IDFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPVLTTPPAVLPPLTALAHLPRLQLNE
EEQESWSCGRRITAHQDQCQPAPKPLASDQQESDPSQTAPNQTDPSANKPMLVVIDCRGEVAGMAYWEDNATVKPKVVFN
ARG
>Mature_322_residues
PFGFVVIDKPAGLTSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSSDDQ
QGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAYARARRGETMEIPARTITIDCLQLLSWNQQLGQI
DFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPVLTTPPAVLPPLTALAHLPRLQLNEE
EQESWSCGRRITAHQDQCQPAPKPLASDQQESDPSQTAPNQTDPSANKPMLVVIDCRGEVAGMAYWEDNATVKPKVVFNA
RG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=32.1428571428571, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI215599015, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4503337, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367200, Length=207, Percent_Identity=37.1980676328502, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553978, Length=196, Percent_Identity=32.1428571428571, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=202, Percent_Identity=30.1980198019802, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=136, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35434; Mature: 35302

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFGFVVIDKPAGLTSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PGAKSYRGSIQLGQRTSSDDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAV
CCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
HVQGERAYARARRGETMEIPARTITIDCLQLLSWNQQLGQIDFNVHCSSGTYIRSLARDL
EEECHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHH
GELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPVLTTPPAVLPPLTALAHLPRLQLNE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC
EEQESWSCGRRITAHQDQCQPAPKPLASDQQESDPSQTAPNQTDPSANKPMLVVIDCRGE
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
VAGMAYWEDNATVKPKVVFNARG
EEEEEEECCCCEECCEEEEECCC
>Mature Secondary Structure 
PFGFVVIDKPAGLTSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PGAKSYRGSIQLGQRTSSDDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAV
CCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
HVQGERAYARARRGETMEIPARTITIDCLQLLSWNQQLGQIDFNVHCSSGTYIRSLARDL
EEECHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHH
GELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPVLTTPPAVLPPLTALAHLPRLQLNE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC
EEQESWSCGRRITAHQDQCQPAPKPLASDQQESDPSQTAPNQTDPSANKPMLVVIDCRGE
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
VAGMAYWEDNATVKPKVVFNARG
EEEEEEECCCCEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]