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The map label for this gene is murD [H]

Identifier: 124022231

GI number: 124022231

Start: 508880

End: 510262

Strand: Reverse

Name: murD [H]

Synonym: P9303_05211

Alternate gene names: 124022231

Gene position: 510262-508880 (Counterclockwise)

Preceding gene: 124022232

Following gene: 124022226

Centisome position: 19.02

GC content: 57.34

Gene sequence:

>1383_bases
ATGGCACGCACTGTGGTCGTGGGGCTTGGACGCTCCGGCATCAGTGCGGCACGACTTCTGCAAGCAGAGGGACATCAGGT
CACAGTGCTTGAGAGCAGCATCGAGCCCCATCTCCAAAGCTTGGCAGCTGATCTACGTCTGCAGGGAATTGCTGTCGAGC
TAGGCAAACCTCTAGAGCTGACCAGCTTCATCCCTTGGCTGGATCAACTTGATTCCGTCGTCATCAGCCCTGGCATCGCC
TGGGACCACCCAACACTGACTGCACTGCGTCAACGCGGCATCGACATCGATGGAGAGATGGCAGTGGCCTGGCGAAGCCT
GAGCCATCTGCCATGGATCGCCATTACCGGCACGAATGGCAAAACAACAGTGACCCACCTGCTCAATCATGTGCTTGAAA
GCAATGGCCTCAAAGCACCAATGGGCGGCAATGTTGGCCATGCCGCGGCTGAAATCGCACTCAAATGGCGGCAACCAAAC
GCACAGCGACCCGACTGGCTGGTCATGGAGCTCAGCAGTTACCAGATCGAAGCCGCCCCTGAGATTGCCCCACGCATTGG
CATTTGGACCAACCTCACCCCCGACCACCTCGAACGCCACGGCACCCTTGATGCTTACCGAAAGATCAAGCGTGGCCTAC
TGGAGCGCTCCGAGATCCGCATCTTCAATGGCGATGATCCAGATCTGAGCAGCCAAAGATCCAGCTGGGATAAGGGGCTG
TGGGTGAGCAGCGAAGGGCCTGGTACTGCAAACCATCGCGCCGACTTTTGGATTGATGCGGAGGGGCTGGTCAGGGACCC
GCAAGGAGCGCTTTTTGCCGCTTCGGCTTTGGCCATGCCTGGACAACACAATCTTCAAAACCTGCTGATGGTGACAGCCG
CAGCCCGACAAACGGGATTGCCAGCTAAGGCCATCGAAGCCAGCCTGCGCTCGTTCCCAGGCGTGCCTCACAGACTCGAA
GAGCTTGGACATATCCAGCAAATGAGCGTCTACAACGACAGCAAGGCCACGAACTATGACGCCGCCTGTGTGGGCCTCAA
GGCCGTCCCTGCTCCTGCCGTTGTGCTGGCAGGTGGGCAAACCAAACAGGGCGATGCTTCTGGATGGCTCAAGCAATTGG
ATCTGAAAGCCTGCGCCGTGATCCTGTTTGGGGCTGGTGCTGCAGAACTAAATGACCTCATCGAAACGTCTGGCTTCCGT
GGTGAGTTGCATTGCTGCAGCAACCTCAACGCTGCTGTGACTTTGGCCATTCCACTGGGCCTAGAGCGACAAGCCGCCTG
CCTGTTGCTTTCACCAGCTTGCGCAAGTTTTGATCAATACCAAGACTTCGAAGCCCGCGGCGAACATTTCCGTAGCCTAA
TTGCACCATATTTAACGGCCTAA

Upstream 100 bases:

>100_bases
CGGGCGATTAGCTCAGAGGTAGAGCACTACCTTGACACGGTAGGAGTCACTGGTTCGATTCCAGTATCGCCCACTCCCCT
TACAAACACAGGACTCATCG

Downstream 100 bases:

>100_bases
TCTACGGCTAACTGATCCCTAGCGTTAACTTCATGACTCATTCCTCTGTTCGATAATGATGCGACTGGCGCTCTATTTGT
AACGGCAATCCTACTTACTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MARTVVVGLGRSGISAARLLQAEGHQVTVLESSIEPHLQSLAADLRLQGIAVELGKPLELTSFIPWLDQLDSVVISPGIA
WDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLKAPMGGNVGHAAAEIALKWRQPN
AQRPDWLVMELSSYQIEAAPEIAPRIGIWTNLTPDHLERHGTLDAYRKIKRGLLERSEIRIFNGDDPDLSSQRSSWDKGL
WVSSEGPGTANHRADFWIDAEGLVRDPQGALFAASALAMPGQHNLQNLLMVTAAARQTGLPAKAIEASLRSFPGVPHRLE
ELGHIQQMSVYNDSKATNYDAACVGLKAVPAPAVVLAGGQTKQGDASGWLKQLDLKACAVILFGAGAAELNDLIETSGFR
GELHCCSNLNAAVTLAIPLGLERQAACLLLSPACASFDQYQDFEARGEHFRSLIAPYLTA

Sequences:

>Translated_460_residues
MARTVVVGLGRSGISAARLLQAEGHQVTVLESSIEPHLQSLAADLRLQGIAVELGKPLELTSFIPWLDQLDSVVISPGIA
WDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLKAPMGGNVGHAAAEIALKWRQPN
AQRPDWLVMELSSYQIEAAPEIAPRIGIWTNLTPDHLERHGTLDAYRKIKRGLLERSEIRIFNGDDPDLSSQRSSWDKGL
WVSSEGPGTANHRADFWIDAEGLVRDPQGALFAASALAMPGQHNLQNLLMVTAAARQTGLPAKAIEASLRSFPGVPHRLE
ELGHIQQMSVYNDSKATNYDAACVGLKAVPAPAVVLAGGQTKQGDASGWLKQLDLKACAVILFGAGAAELNDLIETSGFR
GELHCCSNLNAAVTLAIPLGLERQAACLLLSPACASFDQYQDFEARGEHFRSLIAPYLTA
>Mature_459_residues
ARTVVVGLGRSGISAARLLQAEGHQVTVLESSIEPHLQSLAADLRLQGIAVELGKPLELTSFIPWLDQLDSVVISPGIAW
DHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLKAPMGGNVGHAAAEIALKWRQPNA
QRPDWLVMELSSYQIEAAPEIAPRIGIWTNLTPDHLERHGTLDAYRKIKRGLLERSEIRIFNGDDPDLSSQRSSWDKGLW
VSSEGPGTANHRADFWIDAEGLVRDPQGALFAASALAMPGQHNLQNLLMVTAAARQTGLPAKAIEASLRSFPGVPHRLEE
LGHIQQMSVYNDSKATNYDAACVGLKAVPAPAVVLAGGQTKQGDASGWLKQLDLKACAVILFGAGAAELNDLIETSGFRG
ELHCCSNLNAAVTLAIPLGLERQAACLLLSPACASFDQYQDFEARGEHFRSLIAPYLTA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=29.7592997811816, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49545; Mature: 49414

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVVVGLGRSGISAARLLQAEGHQVTVLESSIEPHLQSLAADLRLQGIAVELGKPLEL
CCCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCH
TSFIPWLDQLDSVVISPGIAWDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNG
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC
KTTVTHLLNHVLESNGLKAPMGGNVGHAAAEIALKWRQPNAQRPDWLVMELSSYQIEAAP
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEEEECCCEEECCC
EIAPRIGIWTNLTPDHLERHGTLDAYRKIKRGLLERSEIRIFNGDDPDLSSQRSSWDKGL
CCCCCCCEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCE
WVSSEGPGTANHRADFWIDAEGLVRDPQGALFAASALAMPGQHNLQNLLMVTAAARQTGL
EEECCCCCCCCCCCEEEECCCCCEECCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHCCC
PAKAIEASLRSFPGVPHRLEELGHIQQMSVYNDSKATNYDAACVGLKAVPAPAVVLAGGQ
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHEECCCCCCCCCHHEEECCCCCCCEEEEECCC
TKQGDASGWLKQLDLKACAVILFGAGAAELNDLIETSGFRGELHCCSNLNAAVTLAIPLG
CCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECC
LERQAACLLLSPACASFDQYQDFEARGEHFRSLIAPYLTA
CCCCCEEEEECHHHHCHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARTVVVGLGRSGISAARLLQAEGHQVTVLESSIEPHLQSLAADLRLQGIAVELGKPLEL
CCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCH
TSFIPWLDQLDSVVISPGIAWDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNG
HHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC
KTTVTHLLNHVLESNGLKAPMGGNVGHAAAEIALKWRQPNAQRPDWLVMELSSYQIEAAP
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEEEECCCEEECCC
EIAPRIGIWTNLTPDHLERHGTLDAYRKIKRGLLERSEIRIFNGDDPDLSSQRSSWDKGL
CCCCCCCEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCCCCE
WVSSEGPGTANHRADFWIDAEGLVRDPQGALFAASALAMPGQHNLQNLLMVTAAARQTGL
EEECCCCCCCCCCCEEEECCCCCEECCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHCCC
PAKAIEASLRSFPGVPHRLEELGHIQQMSVYNDSKATNYDAACVGLKAVPAPAVVLAGGQ
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHEECCCCCCCCCHHEEECCCCCCCEEEEECCC
TKQGDASGWLKQLDLKACAVILFGAGAAELNDLIETSGFRGELHCCSNLNAAVTLAIPLG
CCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECC
LERQAACLLLSPACASFDQYQDFEARGEHFRSLIAPYLTA
CCCCCEEEEECHHHHCHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]