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The map label for this gene is rlmN

Identifier: 124022151

GI number: 124022151

Start: 446601

End: 447671

Strand: Direct

Name: rlmN

Synonym: P9303_04411

Alternate gene names: 124022151

Gene position: 446601-447671 (Clockwise)

Preceding gene: 124022150

Following gene: 124022152

Centisome position: 16.65

GC content: 55.37

Gene sequence:

>1071_bases
GTGAGTTCTACCCATCTGCCTGGCGGCAATCAGGCGTTACTGGGTTGTAGTGCTACTGAACTAGAGAGCTGGGCTGTTGC
AGAAGGCCAGCCAGCCTTCCGGGGCCGTCAGTTGCATGACTGGCTCTATGCCAAGGGGGCCAGAAGTTTTGATGCGATCA
CAGTGTTGCCAAAGTCATGGCGCATCTCTTTGCAACAGCGAGGCCTCACGATTGGGCGGTTGTTGGAGGTGAACCGGGCT
GTTGCGGTGGATGACACGACCAAGTTGCTGCTGGCAACGGTTGATGGCGAAACCATTGAGAGTGTAGGTATCCCAACCCA
GCAACGTCTCACAGTTTGCCTATCCAGTCAGGTGGGCTGTCCAATGGCTTGTCGCTTCTGCGCTAGCGGTAAGGGAGGGC
TGCAACGATCGCTGGCCACACACGAGATTGTTGATCAGGTGTTGAGCTTGCGCGAGGCGATGGATCGCCGTCCTTCCCAC
GTTGTCTTCATGGGTATGGGTGAACCTTTGCTCAACATTGAAGCGGTCTTGGCGTCGATTCGTTGCCTGAATATTGATCT
AGGCATTGCTCAGCGCCGTATCACTGTGAGCACCGTGGGTGTTCCCCATACGCTGCCTCAGCTGGCAGAACTTGCTATGA
AACGTCTAGGTCGCGCCCAGTTCACCCTGGCTGTGAGTCTGCATGCTCCCAATCAAGAGTTGCGCGAACGTTTGATTCCT
ACCGCTTGCGCTTATCCCTTCGAGACTCTTCTTCAAGACTGTCGCCATTATTTGGCAGTGACTGGTCGAAGGGTGACTTT
TGAGTACATCCTGCTTGGAGCACTTAATGATCAGCCTCAGCATGCAGAAGAACTAGCGGAACGGGTTAGGGGCTTCCAGA
GTCATGTGAATCTGATTGCCTACAACCCCATCGACGATGAGGGTTTTCAACGTCCTAATCCAGAGACGATTGAGGCCTTT
CGGCGTGTATTGGAGCAGCGTGGGGTGGCAGTGAGTTTGCGTGCAAGTCGAGGGCTGGATCAGAATGCCGCCTGTGGCCA
GCTCCGCCGCCAGCACGCAGCCATCGGCTGA

Upstream 100 bases:

>100_bases
TTCACAGCCATAACGAGAAGCTCAATGGACGTATGGCGATGCTTGGCTTTATTGCCTTAATGGTGGTGGAGGCAACACTC
GGGCATGGCTTGTTGATCTG

Downstream 100 bases:

>100_bases
GATGGACTAGCTGAGCCACACTGAGATCAACCGCCTAGGGATATGGCTGCCATTGATTGGATAATCCTCGTTGGTTATCT
CGCCATCACTCTCGTGATTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSTHLPGGNQALLGCSATELESWAVAEGQPAFRGRQLHDWLYAKGARSFDAITVLPKSWRISLQQRGLTIGRLLEVNRA
VAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSH
VVFMGMGEPLLNIEAVLASIRCLNIDLGIAQRRITVSTVGVPHTLPQLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELAERVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAF
RRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAAIG

Sequences:

>Translated_356_residues
MSSTHLPGGNQALLGCSATELESWAVAEGQPAFRGRQLHDWLYAKGARSFDAITVLPKSWRISLQQRGLTIGRLLEVNRA
VAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSH
VVFMGMGEPLLNIEAVLASIRCLNIDLGIAQRRITVSTVGVPHTLPQLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELAERVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAF
RRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAAIG
>Mature_355_residues
SSTHLPGGNQALLGCSATELESWAVAEGQPAFRGRQLHDWLYAKGARSFDAITVLPKSWRISLQQRGLTIGRLLEVNRAV
AVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHV
VFMGMGEPLLNIEAVLASIRCLNIDLGIAQRRITVSTVGVPHTLPQLAELAMKRLGRAQFTLAVSLHAPNQELRERLIPT
ACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELAERVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAFR
RVLEQRGVAVSLRASRGLDQNAACGQLRRQHAAIG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=34.2776203966006, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PROM3 (A2C6T3)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016458.1
- ProteinModelPortal:   A2C6T3
- STRING:   A2C6T3
- GeneID:   4777882
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_04411
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14457
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39154; Mature: 39022

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTHLPGGNQALLGCSATELESWAVAEGQPAFRGRQLHDWLYAKGARSFDAITVLPKSW
CCCCCCCCCCCEEEECCHHHHCCEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCHHH
RISLQQRGLTIGRLLEVNRAVAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGC
EEEHHHCCCHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCCCC
PMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHVVFMGMGEPLLNIEAVLASI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
RCLNIDLGIAQRRITVSTVGVPHTLPQLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
HHEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCC
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELAERVRGFQSHVNLIA
HHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEE
YNPIDDEGFQRPNPETIEAFRRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAAIG
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSTHLPGGNQALLGCSATELESWAVAEGQPAFRGRQLHDWLYAKGARSFDAITVLPKSW
CCCCCCCCCCEEEECCHHHHCCEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCHHH
RISLQQRGLTIGRLLEVNRAVAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGC
EEEHHHCCCHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCCCC
PMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHVVFMGMGEPLLNIEAVLASI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
RCLNIDLGIAQRRITVSTVGVPHTLPQLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
HHEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCC
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELAERVRGFQSHVNLIA
HHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEE
YNPIDDEGFQRPNPETIEAFRRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAAIG
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA