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The map label for this gene is nadB [H]

Identifier: 124021931

GI number: 124021931

Start: 235057

End: 236727

Strand: Reverse

Name: nadB [H]

Synonym: P9303_02181

Alternate gene names: 124021931

Gene position: 236727-235057 (Counterclockwise)

Preceding gene: 124021932

Following gene: 124021929

Centisome position: 8.82

GC content: 56.79

Gene sequence:

>1671_bases
ATGAGTCTCTCTAACCCATCCACGCCCATTCCTAACGGTCCCTGGGATGTCGTGGTGATCGGCGCAGGGGCAGCAGGGCT
GATGACCTGCCTTGAGCTGCCTGCTCACCTGAAGGTTCTTTTGCTGAACCGCAACACCAGCCGCCACTCCTCCAGCCGCT
GGGCACAAGGTGGCATCGCCGCAGTAACAAGACCTAAAGACAGCGCCGAGAGTCATGCAGAAGACACTCTCAAAGCAGGC
GCAGGACTCTGTGATGCTGATGCTGTCAGAAAACTTGTAGAAGAAGCTCCCCACTGCGTTCAACGTCTCCAGAAGCTTGG
GATGGCCTTTGACCGCGAAGGTGATGCGCTAGCCACCACCTTGGAGGCAGCCCATAGCCATCGAAGAGTGCTGCATGTGC
AGGACCAAACGGGCCGCGCCTTGGTTGAAGCGCTGCAAGAGCAAGTGGAACGGCGCCCCAACCTCTTGCACCGGCGAGGG
GTACGCGCCACCCAGCTTTGGATTGAGCACAATCGCTGCTACGGAGTACAAGTTCTCGACGGAACGCAGCTCCATTGGCT
TTCGGCCAGAGCCGTAGTCCTGGCAACTGGTGGCGGTGGCCATCTGTTCGCAAACACCACCAATCCGGCTCAGGCCTGCG
GAGAAGGCTTAGCCCTTGCCTGGCAAGCAGGCGCCACGATCGAGGATCTTGAATTCGTGCAGTTTCATCCCACAGCTCTG
AAACTGGAAGGAGCTCCATGCTTTCTGATTTCAGAAGCAGTCAGAGGCGAAGGTGCCGTACTGGTTGATGCCCAGGGGCA
AAGTCCTGTAAAGGAATTGAGCGCTGGTGATCTTGCTCCTCGCGACCAAGTAAGCCGGTCCCTCATCCAAACCATGCATC
ACCAAGGGGTCCAGCATCTTGGCCTAGATCTCACACCCATCCCTTCAGGGCAGGCCGAGGTCCGTTTCCCCACCATCCTG
GAACGCTGTCGGGAATTAGGTTTAGATCCACTCAACCAACCGATCCCGGTAGCCCCAGCAGCTCACTACTGGATGGGAGG
AATCGCCACCAACCTTCAAGCCGCAACCAATCTTCCAGGGCTCTATGCGATTGGGGAAGTCGCCTGCACCGGAGTGCATG
GAGCCAACCGACTCGCTGGCAACTCACTGATGGAATGCATGGTATTTGCAAGACAAATGGCCACAATCGAACTGGGCTCT
TTGCCTCGTCTGGGTGTTCATCAACGAGATTCTTCCCCCGAGCCTTGCTGTCAGCAACTCCATGGAACTGAAAGTGCCGA
GTCTCTAACTCGCGCCATCGCAGATCTTCGAGAGCTTTGCTGGCGAAAGGCGGGTGTAGATCGCTCAGCAAAGGGAATGA
AACAAAGCCTCGCGAGCATCCGTAAGGATCTACAGCACATTGGTGAACAGCCTTTGCTCAAGCTGGTAAGCCATCAGCCC
AAAGAAATTTGCCATCGCCTCGATGACACGGCCAGAAGGGACCTCAACTTGCTACTGGACCTCAACCATCGCCAGTTGAC
CAGCTCCTTACTGCTGGAAGCCTGTTTGTTCCGCCGCGAAAGTCGCGGCGGACATTTCAGAACTGATGCCCCTGCAGCAA
TGCCTCAGTGGAAACGCCACTCACGCCAAAAACGAGGAGAAGACCTCTCCACGCGGCCAGTGAGGTCCTAA

Upstream 100 bases:

>100_bases
ATCGCATCAACGACGGTCAGTACAGATAAACGATTCCTCACTCAACAATCAGCTCAACCGCAAGCCGTCGGTCCATCCGG
CGGCTTTTTTGTAGGTTTCA

Downstream 100 bases:

>100_bases
CTGGCTGCTTCAGAACTGCCTCCCGCCCTGATAGCCACTCATATCAGCAAGCTTATTGAGCGGTTTCACGCCGGATTCGA
GCTCCCCGTTAATTTCCCAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSLSNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAHLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAG
AGLCDADAVRKLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRG
VRATQLWIEHNRCYGVQVLDGTQLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
KLEGAPCFLISEAVRGEGAVLVDAQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTIL
ERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIELGS
LPRLGVHQRDSSPEPCCQQLHGTESAESLTRAIADLRELCWRKAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
KEICHRLDDTARRDLNLLLDLNHRQLTSSLLLEACLFRRESRGGHFRTDAPAAMPQWKRHSRQKRGEDLSTRPVRS

Sequences:

>Translated_556_residues
MSLSNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAHLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAG
AGLCDADAVRKLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRG
VRATQLWIEHNRCYGVQVLDGTQLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
KLEGAPCFLISEAVRGEGAVLVDAQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTIL
ERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIELGS
LPRLGVHQRDSSPEPCCQQLHGTESAESLTRAIADLRELCWRKAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
KEICHRLDDTARRDLNLLLDLNHRQLTSSLLLEACLFRRESRGGHFRTDAPAAMPQWKRHSRQKRGEDLSTRPVRS
>Mature_555_residues
SLSNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAHLKVLLLNRNTSRHSSSRWAQGGIAAVTRPKDSAESHAEDTLKAGA
GLCDADAVRKLVEEAPHCVQRLQKLGMAFDREGDALATTLEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGV
RATQLWIEHNRCYGVQVLDGTQLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTALK
LEGAPCFLISEAVRGEGAVLVDAQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHLGLDLTPIPSGQAEVRFPTILE
RCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPGLYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIELGSL
PRLGVHQRDSSPEPCCQQLHGTESAESLTRAIADLRELCWRKAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQPK
EICHRLDDTARRDLNLLLDLNHRQLTSSLLLEACLFRRESRGGHFRTDAPAAMPQWKRHSRQKRGEDLSTRPVRS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=32.824427480916, Blast_Score=192, Evalue=9e-49,
Organism=Escherichia coli, GI1788928, Length=537, Percent_Identity=35.9404096834264, Blast_Score=302, Evalue=5e-83,
Organism=Escherichia coli, GI1790597, Length=551, Percent_Identity=27.5862068965517, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1786942, Length=573, Percent_Identity=30.7155322862129, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17505833, Length=581, Percent_Identity=30.1204819277108, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17550100, Length=534, Percent_Identity=31.6479400749064, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=421, Percent_Identity=27.0783847980998, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=577, Percent_Identity=31.7157712305026, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322416, Length=585, Percent_Identity=31.4529914529915, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320788, Length=466, Percent_Identity=27.8969957081545, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137288, Length=578, Percent_Identity=32.0069204152249, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655642, Length=578, Percent_Identity=32.0069204152249, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655647, Length=578, Percent_Identity=32.0069204152249, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24663005, Length=520, Percent_Identity=28.8461538461538, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60502; Mature: 60371

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAHLKVLLLNRNTSRHSSSRWAQGGIA
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCEE
AVTRPKDSAESHAEDTLKAGAGLCDADAVRKLVEEAPHCVQRLQKLGMAFDREGDALATT
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
LEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGVRATQLWIEHNRCYGVQVLD
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHEEEECCCEEEEEEEC
GTQLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
CCEEEEEECCEEEEEECCCCEEEECCCCHHHHHCCCCEEEEECCCCHHHCHHEEECCCEE
KLEGAPCFLISEAVRGEGAVLVDAQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHL
EECCCCEEEEHHHHCCCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
GLDLTPIPSGQAEVRFPTILERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPG
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCC
LYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIELGSLPRLGVHQRDSSPEPCCQQL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
HGTESAESLTRAIADLRELCWRKAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
KEICHRLDDTARRDLNLLLDLNHRQLTSSLLLEACLFRRESRGGHFRTDAPAAMPQWKRH
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
SRQKRGEDLSTRPVRS
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SLSNPSTPIPNGPWDVVVIGAGAAGLMTCLELPAHLKVLLLNRNTSRHSSSRWAQGGIA
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCEE
AVTRPKDSAESHAEDTLKAGAGLCDADAVRKLVEEAPHCVQRLQKLGMAFDREGDALATT
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHH
LEAAHSHRRVLHVQDQTGRALVEALQEQVERRPNLLHRRGVRATQLWIEHNRCYGVQVLD
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHEEEECCCEEEEEEEC
GTQLHWLSARAVVLATGGGGHLFANTTNPAQACGEGLALAWQAGATIEDLEFVQFHPTAL
CCEEEEEECCEEEEEECCCCEEEECCCCHHHHHCCCCEEEEECCCCHHHCHHEEECCCEE
KLEGAPCFLISEAVRGEGAVLVDAQGQSPVKELSAGDLAPRDQVSRSLIQTMHHQGVQHL
EECCCCEEEEHHHHCCCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHH
GLDLTPIPSGQAEVRFPTILERCRELGLDPLNQPIPVAPAAHYWMGGIATNLQAATNLPG
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCC
LYAIGEVACTGVHGANRLAGNSLMECMVFARQMATIELGSLPRLGVHQRDSSPEPCCQQL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
HGTESAESLTRAIADLRELCWRKAGVDRSAKGMKQSLASIRKDLQHIGEQPLLKLVSHQP
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCH
KEICHRLDDTARRDLNLLLDLNHRQLTSSLLLEACLFRRESRGGHFRTDAPAAMPQWKRH
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
SRQKRGEDLSTRPVRS
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]