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The map label for this gene is rimO

Identifier: 124021929

GI number: 124021929

Start: 232579

End: 233997

Strand: Reverse

Name: rimO

Synonym: P9303_02161

Alternate gene names: 124021929

Gene position: 233997-232579 (Counterclockwise)

Preceding gene: 124021931

Following gene: 124021928

Centisome position: 8.72

GC content: 57.36

Gene sequence:

>1419_bases
ATGACCAAGCCTGCGTTGCGATCCGACATACCAATGAAGCCCACAGCCCACAAACAAGAAAAACCATCGGTGGCCTTTGC
CCATTTGGGCTGTGAGAAAAATCGCGTTGACACCGAACACATGCTGGGACTGCTTACAGAAGCGGGCTATAGCGTCAGCA
GCGATGAAAACGATGCCGCCGTCGTGGTGGTCAACACCTGCAGCTTCATCCAGGACGCGCGCGAGGAATCGGTACGGACG
TTGATTGGACTAGCAGAACAGGGCAAGGAACTGATCATCGCCGGATGCCTTGCCCAGCATTTTCAAGAGGAACTGCTCGA
GTCAATCCCGGAGGCAAAAGCAATCGTCGGCACCGGTGACTATCAGCACATCGTGGATGTGCTGAAGCGAGTAGAAGCGG
GTGAACGGGTGAACCGTGTCAGCGCTTTCCCCACGTTTGTGGGCGATGAGACTCTCCCCCGGCAGCGCACCACTGATCAG
GCTGTGGCTTATCTGAAAGTTGCTGAGGGATGTGATTACCGCTGTGCCTTCTGCATCATCCCAAAGCTGCGTGGCGACCA
GCGCAGCCGACCGGTGGAGTCGATTGTGGCAGAGGCGCACCAGCTGGCTGAGCAGGGGGTGCAGGAACTAATCCTGATCA
GCCAAATAACAACCAACTATGGCCTTGACCTCTATGGCAAGCCCAAATTTGCAGAGCTGCTACAGGCGCTGGGGGAGGTA
GACATCCCCTGGGTGAGGGTGCATTACGCCTACCCCACTGGCCTCACTCCAGAGGTGCTGGCGGCCTATCGGGAGGTGCC
AAATGTGCTTCGCTATCTCGACCTTCCACTGCAACACAGCCATCCAGATGTGCTACGGGCTATGAACCGTCCCTGGCAAA
CGGATGTGAACGAACGGCTATTGGATCGGATTCGTGAGCAGCTACCAGACGCAGTCCTGCGTACCACGCTCATCGTCGGC
TTCCCCGGGGAAACTGAAGATCACTTCAATCACCTCGCCGCCTTCATCGAACGGCAGCGTTTTGATCACGTTGGCGTGTT
CACCTTTTCTCCAGAAGATGGAACCGCCGCAGCCGATCTGCCAAACAGGGTAGACCCGTCAATCGCCGCAGCCCGCAAGG
ATCGATTGATGGCGCTTCAGCAACCCATCTCAGCGGAAAGGAACCAACGATGGGTGGGACGCACCATCGATGTGCTGATC
GAGCAGCACAACCCCGAAACCGGAGCGATGATTGGCCGCTGCGATCGTTTCGCTCCGGAGGTCGATGGCGAGGTGTTGGT
ACTGCCTAGTGAGAAAGGTCTGCAGGCCAGTCCAGGAACGATGGTTCCTGTCTTCATTACCGGCTCTGATGTCTATGACC
TCACCGGCCAGCTGGTGGACACCAACGCCATGGCGGTCACAGCCCAGACGTCTCAGTGA

Upstream 100 bases:

>100_bases
CACAGATCTAAGAATTGTGGCAGCTCTGGAAGAAGCCACCCTCTCTTTAGGGTGGCGCCTAGCGGGAACTGCCTAGGCAG
CCCAGAAATCAACCCTTTCC

Downstream 100 bases:

>100_bases
GTCCATTCCCCTGTTTGCGAGCCCATCAACGCAACATCTTTCTGACAGCCTCTGGCATCAGTACAGCAGGCTCGTTCGCT
GGAATGACTGCAAAAGGCTG

Product: Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTKPALRSDIPMKPTAHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRT
LIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNRVSAFPTFVGDETLPRQRTTDQ
AVAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVAEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPDVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVG
FPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPNRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLI
EQHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ

Sequences:

>Translated_472_residues
MTKPALRSDIPMKPTAHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRT
LIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNRVSAFPTFVGDETLPRQRTTDQ
AVAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVAEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPDVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVG
FPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPNRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLI
EQHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
>Mature_471_residues
TKPALRSDIPMKPTAHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAAVVVVNTCSFIQDAREESVRTL
IGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGDYQHIVDVLKRVEAGERVNRVSAFPTFVGDETLPRQRTTDQA
VAYLKVAEGCDYRCAFCIIPKLRGDQRSRPVESIVAEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEVD
IPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPDVLRAMNRPWQTDVNERLLDRIREQLPDAVLRTTLIVGF
PGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADLPNRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIE
QHNPETGAMIGRCDRFAPEVDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=27.5789473684211, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=27.5789473684211, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI93277076, Length=344, Percent_Identity=28.1976744186047, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=38.6666666666667, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1786882, Length=385, Percent_Identity=30.3896103896104, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=477, Percent_Identity=25.3668763102725, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=325, Percent_Identity=25.8461538461538, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356207, Length=434, Percent_Identity=29.2626728110599, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=28.6016949152542, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PROM3 (A2C661)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016236.1
- ProteinModelPortal:   A2C661
- SMR:   A2C661
- STRING:   A2C661
- GeneID:   4776433
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_02161
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK893584
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52288; Mature: 52157

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPALRSDIPMKPTAHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAA
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VVVVNTCSFIQDAREESVRTLIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGD
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCC
YQHIVDVLKRVEAGERVNRVSAFPTFVGDETLPRQRTTDQAVAYLKVAEGCDYRCAFCII
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
PKLRGDQRSRPVESIVAEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPDVLRAMNRPWQTDVNERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHH
LDRIREQLPDAVLRTTLIVGFPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADL
HHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
PNRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIEQHNPETGAMIGRCDRFAPE
CCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
CCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCEEECCEEEEEEECCC
>Mature Secondary Structure 
TKPALRSDIPMKPTAHKQEKPSVAFAHLGCEKNRVDTEHMLGLLTEAGYSVSSDENDAA
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VVVVNTCSFIQDAREESVRTLIGLAEQGKELIIAGCLAQHFQEELLESIPEAKAIVGTGD
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCC
YQHIVDVLKRVEAGERVNRVSAFPTFVGDETLPRQRTTDQAVAYLKVAEGCDYRCAFCII
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
PKLRGDQRSRPVESIVAEAHQLAEQGVQELILISQITTNYGLDLYGKPKFAELLQALGEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
DIPWVRVHYAYPTGLTPEVLAAYREVPNVLRYLDLPLQHSHPDVLRAMNRPWQTDVNERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHH
LDRIREQLPDAVLRTTLIVGFPGETEDHFNHLAAFIERQRFDHVGVFTFSPEDGTAAADL
HHHHHHHCCHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
PNRVDPSIAAARKDRLMALQQPISAERNQRWVGRTIDVLIEQHNPETGAMIGRCDRFAPE
CCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
VDGEVLVLPSEKGLQASPGTMVPVFITGSDVYDLTGQLVDTNAMAVTAQTSQ
CCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA