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The map label for this gene is ygcM [C]

Identifier: 124021919

GI number: 124021919

Start: 224666

End: 225586

Strand: Direct

Name: ygcM [C]

Synonym: P9303_02061

Alternate gene names: 124021919

Gene position: 224666-225586 (Clockwise)

Preceding gene: 124021916

Following gene: 124021920

Centisome position: 8.37

GC content: 50.92

Gene sequence:

>921_bases
ATGACTGACGTCCCCGATTTGGAACGCCATGGTCATGGCCGCGGCTGTGTGATCACTCGCAAAGCTAGGTTCAGTGCTGG
CCATCGCTATTACTTACCGGAACTGTCAAGCGATGACAATGCAGCTCGTTTTGGACTCTGTGCTTTGCCTGCTGGGCATG
GTCACAATTATGAGCTGATCGTCGCCATGGCTGGCGGGCTTAATGTCGATGGCATGGTGTTGAACCTCTCTGAGGTGAAG
CACGCCATCCGTTCTGAGGTAACCGATCAGCTTGACTTTCGCTTCCTTAACGATGTTTGGCCGGAATTTGATCTTTCCAG
GCCAGAGGGCTGCCTGCCGACCACGGAGGCTCTGCTAAGAATCATCTGGACTCGTTTAATTGCTCATCTTCCAATCGTGG
CCATTCGGCTTTACGAACAAGAAAACCTCTGGGCTGATTATCACGGAGATGCCATGGACGCCTATCTAACGATCCGTACC
CATTTTGCGGCTGCCCATCGGCTTGCCAGGGTCGAACTGAGTCAGGAGGAAAACGAACGTATTTATGGCAAATGCGCGCG
GCCTCATGGGCATGGTCATAATTATTTTGTGGATGTCACCGTGCGCGGAGAAATTGATCCCCGAACAGGCATGGTCTGTG
ATCTAGGTGCTTTGCAAAGCCTTGTGAATTGCATTGTTGTGGAACCTTTTGACCACACCTTCTTAAATAAAGACATTCCC
CATTTCGCTAACTGTGTACCAACGGCAGAGAATATTGCCTTGTACATTTCTGATTGTTTAGCGAAACCGATCCGAGCGAT
CGGAGCCCATCTCCACAAGGTGCGGTTGCAGGAAAGCCCTAATAATGCAGCTGAAGTCTATGCGGAAACCCCAAGGATTG
AAGCCATCCCTGAGGTATTGCATGCTATGGCGGCTCACTAG

Upstream 100 bases:

>100_bases
ACCGCCAAGCCTTTGCTTGAGAGGATGAGGGGCTGGGCTTTCGTTCATTGGTTCGTAAATGTCTATAGATCTCTAGGATG
ACTTCTAGTCCGAGAGAGCG

Downstream 100 bases:

>100_bases
CCAAGCATTTGCAAAAGCAGTTTTGGCTTCGTCTTGTCCTAGCGATCAGCTTGGATGGCCGGCTGGCGCCTGCGATTGGT
GGGCCGGCCCAATTGGGTGG

Product: 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTDVPDLERHGHGRGCVITRKARFSAGHRYYLPELSSDDNAARFGLCALPAGHGHNYELIVAMAGGLNVDGMVLNLSEVK
HAIRSEVTDQLDFRFLNDVWPEFDLSRPEGCLPTTEALLRIIWTRLIAHLPIVAIRLYEQENLWADYHGDAMDAYLTIRT
HFAAAHRLARVELSQEENERIYGKCARPHGHGHNYFVDVTVRGEIDPRTGMVCDLGALQSLVNCIVVEPFDHTFLNKDIP
HFANCVPTAENIALYISDCLAKPIRAIGAHLHKVRLQESPNNAAEVYAETPRIEAIPEVLHAMAAH

Sequences:

>Translated_306_residues
MTDVPDLERHGHGRGCVITRKARFSAGHRYYLPELSSDDNAARFGLCALPAGHGHNYELIVAMAGGLNVDGMVLNLSEVK
HAIRSEVTDQLDFRFLNDVWPEFDLSRPEGCLPTTEALLRIIWTRLIAHLPIVAIRLYEQENLWADYHGDAMDAYLTIRT
HFAAAHRLARVELSQEENERIYGKCARPHGHGHNYFVDVTVRGEIDPRTGMVCDLGALQSLVNCIVVEPFDHTFLNKDIP
HFANCVPTAENIALYISDCLAKPIRAIGAHLHKVRLQESPNNAAEVYAETPRIEAIPEVLHAMAAH
>Mature_305_residues
TDVPDLERHGHGRGCVITRKARFSAGHRYYLPELSSDDNAARFGLCALPAGHGHNYELIVAMAGGLNVDGMVLNLSEVKH
AIRSEVTDQLDFRFLNDVWPEFDLSRPEGCLPTTEALLRIIWTRLIAHLPIVAIRLYEQENLWADYHGDAMDAYLTIRTH
FAAAHRLARVELSQEENERIYGKCARPHGHGHNYFVDVTVRGEIDPRTGMVCDLGALQSLVNCIVVEPFDHTFLNKDIPH
FANCVPTAENIALYISDCLAKPIRAIGAHLHKVRLQESPNNAAEVYAETPRIEAIPEVLHAMAAH

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=129, Percent_Identity=48.062015503876, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=42.9752066115703, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=42.9752066115703, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19550074, Length=129, Percent_Identity=43.4108527131783, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585378, Length=129, Percent_Identity=43.4108527131783, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 34233; Mature: 34102

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPDLERHGHGRGCVITRKARFSAGHRYYLPELSSDDNAARFGLCALPAGHGHNYELI
CCCCCCHHHCCCCCCEEEEECHHCCCCCEEECCCCCCCCCCHHEEEEECCCCCCCCEEEE
VAMAGGLNVDGMVLNLSEVKHAIRSEVTDQLDFRFLNDVWPEFDLSRPEGCLPTTEALLR
EEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
IIWTRLIAHLPIVAIRLYEQENLWADYHGDAMDAYLTIRTHFAAAHRLARVELSQEENER
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
IYGKCARPHGHGHNYFVDVTVRGEIDPRTGMVCDLGALQSLVNCIVVEPFDHTFLNKDIP
EEEEECCCCCCCCCEEEEEEEECCCCCCCCCEECHHHHHHHHHEEEECCCCCHHCCCCCC
HFANCVPTAENIALYISDCLAKPIRAIGAHLHKVRLQESPNNAAEVYAETPRIEAIPEVL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHEEECCCCHHHHHHHH
HAMAAH
HHHHCC
>Mature Secondary Structure 
TDVPDLERHGHGRGCVITRKARFSAGHRYYLPELSSDDNAARFGLCALPAGHGHNYELI
CCCCCHHHCCCCCCEEEEECHHCCCCCEEECCCCCCCCCCHHEEEEECCCCCCCCEEEE
VAMAGGLNVDGMVLNLSEVKHAIRSEVTDQLDFRFLNDVWPEFDLSRPEGCLPTTEALLR
EEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
IIWTRLIAHLPIVAIRLYEQENLWADYHGDAMDAYLTIRTHFAAAHRLARVELSQEENER
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
IYGKCARPHGHGHNYFVDVTVRGEIDPRTGMVCDLGALQSLVNCIVVEPFDHTFLNKDIP
EEEEECCCCCCCCCEEEEEEEECCCCCCCCCEECHHHHHHHHHEEEECCCCCHHCCCCCC
HFANCVPTAENIALYISDCLAKPIRAIGAHLHKVRLQESPNNAAEVYAETPRIEAIPEVL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHEEECCCCHHHHHHHH
HAMAAH
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA