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The map label for this gene is miaA

Identifier: 124021869

GI number: 124021869

Start: 173508

End: 174407

Strand: Reverse

Name: miaA

Synonym: P9303_01561

Alternate gene names: 124021869

Gene position: 174407-173508 (Counterclockwise)

Preceding gene: 124021875

Following gene: 124021868

Centisome position: 6.5

GC content: 57.11

Gene sequence:

>900_bases
ATGCCCGCCTCTAAGCCGCTTCTGATTGCGCTGCTAGGGCCGACAGCAAGCGGCAAAACATGCCTGGCCTTACAGCTGGC
AGAACAGCTGAAGCTGAGCGTGCTCAATGTGGATTCCCGCCAGCTCTACATCGGCATGGACGTTGGCACCGCCAAGCCAA
CCAAAGAACAACAGCGGCGCGTGCAACATCACCTGATTGATCTTCGTCGCCCAGATCAACCGATCACCCTGCAGGAATTC
CAGGCAGCCGCGCAACTGATCCTTGCGCAAAAGCTAAGGGAACAAAACATGCCATTTCTAGTTGGCGGCAGTGGTCTTTA
TCTCAAAGCTCTCACTTGTGGGCTGCGCCCACCAGCAGTGCCACCCCAACCAGAGCTCCGCAAGCAGTTAGGCGAGCTAG
GACAACGCACCTGCCACCACCTACTCCAAGCCGCCGATCCCACCGCTGCCAGCCGAATTGCCCCTGCTGATGCCATGCGC
ACCAAACGGGCTCTGGAAGTGGTTTATGCCACTGGCAAACCGATCACCACTCAACAAGGCTCAAGCCCACCCCCTTGGCG
CGTTCTGGAACTAGGCCTCGACCCACACAACCTGCGCGAACGCATTGGCCAACGCACAACGCAGCTCTATGCCAATGGTC
TCATCGAAGAAACCGAACACTTGAGTCAGTGCTACGACAGCGATCTGCCCTTGCTGCAAACAATCGGCTATGGGGAAGCA
CTAAAGGTGATTCAAGGCCTACTCAACCGTGAACAGGCCATCGCCCTGACCACACGTCGTACTCAGCAGTTCGCCAAGCG
TCAGCGCACATGGTTCCGCAGACAACATCACCCCTATTGGCTCAAAGGTGAGGAACCGCTGAGCGAAGCGCTATCACTGA
TTCAAGCGGGTCTAAGGTGA

Upstream 100 bases:

>100_bases
ACCACTGGCTCACCACTGGCGCTTACAACGGTCACTGAAGCGTCATTGCCCAAAAGGGACGACCTAAACGTTGCTGACAT
GACACTCATCACAGCTGCTC

Downstream 100 bases:

>100_bases
AGGGTGCAGACATAAAACCTTCTGGTCTCCTTGTCGTCTGCTCTATTCCCTTATCTCACTGAATGCCACCACGTCCTCGT
TTTGACCGTCGCGCACCCGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEF
QAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAASRIAPADAMR
TKRALEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGQRTTQLYANGLIEETEHLSQCYDSDLPLLQTIGYGEA
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR

Sequences:

>Translated_299_residues
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEF
QAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAASRIAPADAMR
TKRALEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGQRTTQLYANGLIEETEHLSQCYDSDLPLLQTIGYGEA
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
>Mature_298_residues
PASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRRVQHHLIDLRRPDQPITLQEFQ
AAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAVPPQPELRKQLGELGQRTCHHLLQAADPTAASRIAPADAMRT
KRALEVVYATGKPITTQQGSSPPPWRVLELGLDPHNLRERIGQRTTQLYANGLIEETEHLSQCYDSDLPLLQTIGYGEAL
KVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=25.0793650793651, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=38.4353741496599, Blast_Score=183, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324848, Length=308, Percent_Identity=26.2987012987013, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24649830, Length=264, Percent_Identity=32.5757575757576, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROM3 (A2C601)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016176.1
- ProteinModelPortal:   A2C601
- SMR:   A2C601
- STRING:   A2C601
- GeneID:   4776669
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_01561
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33390; Mature: 33259

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRR
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
VQHHLIDLRRPDQPITLQEFQAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHCCCCCCC
PPQPELRKQLGELGQRTCHHLLQAADPTAASRIAPADAMRTKRALEVVYATGKPITTQQG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHEEECCCCEECCCC
SSPPPWRVLELGLDPHNLRERIGQRTTQLYANGLIEETEHLSQCYDSDLPLLQTIGYGEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHH
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PASKPLLIALLGPTASGKTCLALQLAEQLKLSVLNVDSRQLYIGMDVGTAKPTKEQQRR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
VQHHLIDLRRPDQPITLQEFQAAAQLILAQKLREQNMPFLVGGSGLYLKALTCGLRPPAV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHCCCCCCC
PPQPELRKQLGELGQRTCHHLLQAADPTAASRIAPADAMRTKRALEVVYATGKPITTQQG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHEEECCCCEECCCC
SSPPPWRVLELGLDPHNLRERIGQRTTQLYANGLIEETEHLSQCYDSDLPLLQTIGYGEA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHH
LKVIQGLLNREQAIALTTRRTQQFAKRQRTWFRRQHHPYWLKGEEPLSEALSLIQAGLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA