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The map label for this gene is ilvB [H]

Identifier: 124021857

GI number: 124021857

Start: 158274

End: 160001

Strand: Direct

Name: ilvB [H]

Synonym: P9303_01441

Alternate gene names: 124021857

Gene position: 158274-160001 (Clockwise)

Preceding gene: 124021856

Following gene: 124021858

Centisome position: 5.9

GC content: 48.15

Gene sequence:

>1728_bases
ATGCACCTCCTAAATTCAGCCAGGCAGACGATGACTTGTGTGCCTGTGGTTCACGAAGTTGCAGCAGTTGTGGCTGCTGA
GTATCACAATGCAACCGTCTCATCTGGCAAGGCAGCCAAGCCAAGGGGGCGAGCCATTGCTCTGGTTACAGCAGGCCCGG
GATTAACCAATGCTCTGACGGGCCTCGCTGGGGCTTGGTTAGAAAGCCGTGAGCTACTTCTTCTTGGTGGTCAGGCGAAA
GTGGCGGATCTTGCTTCGTCTGGTTTGCGCCAATTGGGCATTCAGGAGGTGAATGGTGTTGCCATGGCCGCTCCCGTTTG
CAAAAGGAGCCTTTGCTTGCGAGAACCATTGGAACAACAAGCATTTTTTGCTGAAGTCTCTTCAGGCTGGCAGGCTCGTC
CTGGCCCTGTATTCCTAGAGTTTCCGCTTGATGTTCAAGCATTGCAAGTTCCTGAAGCTTGGGTCGCTGGCATCGATGAA
GCAAATATCAACAAGGCTGATGACGCTGCTCCTGTCATCGACAGTGACATGGTTCATTCATTAGCAGCTTCTATAGCCGC
GGCTGAGCGTCCTGTCTTGCTGCTTGGAGGGGGGATCTCTTCTGTAACGGCTCAGCAGTTAGAGCCTCAGCTTGCTTCGC
TTGGTTTGCCAGTAATGACGACGTGGAATGGAGCCGATCGCTATGGCGCAGAGCATTCCAATTATTTTGGCAGGCCCAAT
ACCTGGGGACAGCGCTATAGCAATCTGCTGATTCAGCAATCAGATTTTTTGGTTGCGATCGGCAGCCGGCTTGGGCTCCA
GCAGACAGGCTTTAATTGGCAAGAATTTGTGCCAGTTGGGAAAGTTATTCAGGTTGACATTGATCCAGCTGAGCTGGCTA
AGCCCAATCCAAAACTTGATCTAGCCATTGAAGCGAATGCTAATGATTTTATACAACAGTTGCTTAGTTTTGACTTAGGT
AGCCACCCTGATTGGCTTGCTTACTGTTCTGATGTTAAAACCAGATTGCCGATTTCTGAAGCCTGCAATATCACACCTGT
TGGTTATCTCAATCCCTTTGAAATGGTAATCAAGCTATCCATGCTTTGCAATGCTTCTGATCACATTGTTCCTTGTAGTA
GTGGAGGAGCATTCACTGTAATGATGCAGGCTTTTGAGCTTCAGCAGGGTCAGACAATGATCACCGATAAGGGATTAGCG
AGCATGGGGTATGGCTTATCAGGTGCTATCGGAACATCAATTGCTGACCCTGATGTACGTACAGTGCTAGTTGAGGGCGA
TGGTGGATTCACCCAGAACCTTCAGGAGTTAGCAACTGTGGCCGTGAATAATCTCAATCTAAAGATGTTCCTGTTTTGCA
ATAATGGTTATGCATCGATCAGGATGACGCAAAAGAATTACTTTGATGGTGCTTATATGGGTTGTGATGTTTCTTCGGGT
TTAGGCTTCCCTGATTGGTCTAAACTTGCCGAGGCTTATGGGATTGATTGCTTTGAGCTAGGAGAGGCTTGGTGGGATGC
TGAACGATTTGACCATTTGTGGAATCACCAAGGCCCTGCTCTGTTTTTGGTTCCATTGCATCCTGAACAGACATATTCTC
CTAAGATCGCTAGTCGCATTAGTGCTAATGGCGGCATGGAATCAAATCCCTTGCATCGAATGAGTCCGGATTTAGATCAA
GAGCTTGAGGATTTCGTGACACGCTTTATTCCAAAAAAAGCGTCTTAA

Upstream 100 bases:

>100_bases
CTTTAGCCAATCGATTATGATGAAGTATTCAGATGTTGTTTTTCAATGGCTTAAACGGAGTGGCTATAGCCATTGTTACT
TTGTGGCGGGTGGCAATATT

Downstream 100 bases:

>100_bases
CTCCCTTACTATTTTGTTATTTGTGCTATAATAAATATATTCTGGATTGCTTTTGGCATGAATGTTCATGAGCGCAAGAT
GCTTGATATCCTGCGCCAAG

Product: thiamine pyrophosphate-requiring enzyme

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MHLLNSARQTMTCVPVVHEVAAVVAAEYHNATVSSGKAAKPRGRAIALVTAGPGLTNALTGLAGAWLESRELLLLGGQAK
VADLASSGLRQLGIQEVNGVAMAAPVCKRSLCLREPLEQQAFFAEVSSGWQARPGPVFLEFPLDVQALQVPEAWVAGIDE
ANINKADDAAPVIDSDMVHSLAASIAAAERPVLLLGGGISSVTAQQLEPQLASLGLPVMTTWNGADRYGAEHSNYFGRPN
TWGQRYSNLLIQQSDFLVAIGSRLGLQQTGFNWQEFVPVGKVIQVDIDPAELAKPNPKLDLAIEANANDFIQQLLSFDLG
SHPDWLAYCSDVKTRLPISEACNITPVGYLNPFEMVIKLSMLCNASDHIVPCSSGGAFTVMMQAFELQQGQTMITDKGLA
SMGYGLSGAIGTSIADPDVRTVLVEGDGGFTQNLQELATVAVNNLNLKMFLFCNNGYASIRMTQKNYFDGAYMGCDVSSG
LGFPDWSKLAEAYGIDCFELGEAWWDAERFDHLWNHQGPALFLVPLHPEQTYSPKIASRISANGGMESNPLHRMSPDLDQ
ELEDFVTRFIPKKAS

Sequences:

>Translated_575_residues
MHLLNSARQTMTCVPVVHEVAAVVAAEYHNATVSSGKAAKPRGRAIALVTAGPGLTNALTGLAGAWLESRELLLLGGQAK
VADLASSGLRQLGIQEVNGVAMAAPVCKRSLCLREPLEQQAFFAEVSSGWQARPGPVFLEFPLDVQALQVPEAWVAGIDE
ANINKADDAAPVIDSDMVHSLAASIAAAERPVLLLGGGISSVTAQQLEPQLASLGLPVMTTWNGADRYGAEHSNYFGRPN
TWGQRYSNLLIQQSDFLVAIGSRLGLQQTGFNWQEFVPVGKVIQVDIDPAELAKPNPKLDLAIEANANDFIQQLLSFDLG
SHPDWLAYCSDVKTRLPISEACNITPVGYLNPFEMVIKLSMLCNASDHIVPCSSGGAFTVMMQAFELQQGQTMITDKGLA
SMGYGLSGAIGTSIADPDVRTVLVEGDGGFTQNLQELATVAVNNLNLKMFLFCNNGYASIRMTQKNYFDGAYMGCDVSSG
LGFPDWSKLAEAYGIDCFELGEAWWDAERFDHLWNHQGPALFLVPLHPEQTYSPKIASRISANGGMESNPLHRMSPDLDQ
ELEDFVTRFIPKKAS
>Mature_575_residues
MHLLNSARQTMTCVPVVHEVAAVVAAEYHNATVSSGKAAKPRGRAIALVTAGPGLTNALTGLAGAWLESRELLLLGGQAK
VADLASSGLRQLGIQEVNGVAMAAPVCKRSLCLREPLEQQAFFAEVSSGWQARPGPVFLEFPLDVQALQVPEAWVAGIDE
ANINKADDAAPVIDSDMVHSLAASIAAAERPVLLLGGGISSVTAQQLEPQLASLGLPVMTTWNGADRYGAEHSNYFGRPN
TWGQRYSNLLIQQSDFLVAIGSRLGLQQTGFNWQEFVPVGKVIQVDIDPAELAKPNPKLDLAIEANANDFIQQLLSFDLG
SHPDWLAYCSDVKTRLPISEACNITPVGYLNPFEMVIKLSMLCNASDHIVPCSSGGAFTVMMQAFELQQGQTMITDKGLA
SMGYGLSGAIGTSIADPDVRTVLVEGDGGFTQNLQELATVAVNNLNLKMFLFCNNGYASIRMTQKNYFDGAYMGCDVSSG
LGFPDWSKLAEAYGIDCFELGEAWWDAERFDHLWNHQGPALFLVPLHPEQTYSPKIASRISANGGMESNPLHRMSPDLDQ
ELEDFVTRFIPKKAS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=509, Percent_Identity=27.7013752455796, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87081685, Length=501, Percent_Identity=28.1437125748503, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786717, Length=475, Percent_Identity=25.2631578947368, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=516, Percent_Identity=25.1937984496124, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922626, Length=430, Percent_Identity=23.7209302325581, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61986; Mature: 61986

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLNSARQTMTCVPVVHEVAAVVAAEYHNATVSSGKAAKPRGRAIALVTAGPGLTNALT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
GLAGAWLESRELLLLGGQAKVADLASSGLRQLGIQEVNGVAMAAPVCKRSLCLREPLEQQ
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCHHHHCCEEHHHHHHHHHHHHHCHHHHH
AFFAEVSSGWQARPGPVFLEFPLDVQALQVPEAWVAGIDEANINKADDAAPVIDSDMVHS
HHHHHHHCCCCCCCCCEEEECCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LAASIAAAERPVLLLGGGISSVTAQQLEPQLASLGLPVMTTWNGADRYGAEHSNYFGRPN
HHHHHHHCCCCEEEEECCCHHHHHHHHCHHHHHCCCCEEEECCCCHHCCCCCCCCCCCCC
TWGQRYSNLLIQQSDFLVAIGSRLGLQQTGFNWQEFVPVGKVIQVDIDPAELAKPNPKLD
CHHHHHHHHHEECCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCHHHHCCCCCCEE
LAIEANANDFIQQLLSFDLGSHPDWLAYCSDVKTRLPISEACNITPVGYLNPFEMVIKLS
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHH
MLCNASDHIVPCSSGGAFTVMMQAFELQQGQTMITDKGLASMGYGLSGAIGTSIADPDVR
HHHCCCCCEEECCCCCEEEEHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCEE
TVLVEGDGGFTQNLQELATVAVNNLNLKMFLFCNNGYASIRMTQKNYFDGAYMGCDVSSG
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCEECCCCCCC
LGFPDWSKLAEAYGIDCFELGEAWWDAERFDHLWNHQGPALFLVPLHPEQTYSPKIASRI
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHH
SANGGMESNPLHRMSPDLDQELEDFVTRFIPKKAS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHLLNSARQTMTCVPVVHEVAAVVAAEYHNATVSSGKAAKPRGRAIALVTAGPGLTNALT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
GLAGAWLESRELLLLGGQAKVADLASSGLRQLGIQEVNGVAMAAPVCKRSLCLREPLEQQ
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCHHHHCCEEHHHHHHHHHHHHHCHHHHH
AFFAEVSSGWQARPGPVFLEFPLDVQALQVPEAWVAGIDEANINKADDAAPVIDSDMVHS
HHHHHHHCCCCCCCCCEEEECCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LAASIAAAERPVLLLGGGISSVTAQQLEPQLASLGLPVMTTWNGADRYGAEHSNYFGRPN
HHHHHHHCCCCEEEEECCCHHHHHHHHCHHHHHCCCCEEEECCCCHHCCCCCCCCCCCCC
TWGQRYSNLLIQQSDFLVAIGSRLGLQQTGFNWQEFVPVGKVIQVDIDPAELAKPNPKLD
CHHHHHHHHHEECCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCHHHHCCCCCCEE
LAIEANANDFIQQLLSFDLGSHPDWLAYCSDVKTRLPISEACNITPVGYLNPFEMVIKLS
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHH
MLCNASDHIVPCSSGGAFTVMMQAFELQQGQTMITDKGLASMGYGLSGAIGTSIADPDVR
HHHCCCCCEEECCCCCEEEEHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCEE
TVLVEGDGGFTQNLQELATVAVNNLNLKMFLFCNNGYASIRMTQKNYFDGAYMGCDVSSG
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCEECCCCCCC
LGFPDWSKLAEAYGIDCFELGEAWWDAERFDHLWNHQGPALFLVPLHPEQTYSPKIASRI
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHH
SANGGMESNPLHRMSPDLDQELEDFVTRFIPKKAS
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]