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The map label for this gene is hisF [H]

Identifier: 124021820

GI number: 124021820

Start: 106451

End: 107953

Strand: Reverse

Name: hisF [H]

Synonym: P9303_01071

Alternate gene names: 124021820

Gene position: 107953-106451 (Counterclockwise)

Preceding gene: 124021821

Following gene: 124021806

Centisome position: 4.02

GC content: 36.53

Gene sequence:

>1503_bases
ATGGGAAGGCAATCAAGCAAAAATACAGTAAAATATATGGCACTATCTAAAAGAATTATTGCACGATTAGATGTGAAGGG
AAATAAGATCATTAAAGGTGTCCGTTTTGAAGGCCTACGAGTAATTGGCGATCCGTTAGATATGGCAAGAAAGTATGCAA
AGGCTGGTATTGATGAACTGCTTTATATTGATGCTGTTGCAAGCTTATATGGCAGAAATAGTCTTATCGAAGTATTGAAA
AGCACCTCATCAGAAGTCTTCATACCAATTACTGCAGGAGGAGGCATTAGAAATCTAGAAGATGCAAAGTTACTTTTATC
TAATGGGGCGGACAAAATTGCGATTAACACTGCGGCGCTGGAGTGCCCCCAATTGATATCAGAACTTTCTAACACCTTAG
GAAGTCAATGTGTAGTTGTATCTATTCAGGCTAGAGCTTCATCAAGCTCATCATGGGACGCAATGAAAGAATCTGGAAGG
GAAAAGTCAGGTATTGATGTCATAAAATGGATTGAAAATGTACAACTTTTAGGAGCAGGAGAGATATTATTGACTTCAGT
AGATCAAGATGGGACATGTATAAAGCCAGACGAAATATTAATTCAGAAAGTACTTCCTATCCTGAAACTACCTTTAATCG
TAAGCGGAGGTTTTTCTGATCCTAATCAAGTCTATCAAGCATTAACTAATTCCAGGATATCAGCAGTAGCGATTGGAGCC
GCTTTGCATTATCAAAAGACTAATGTATCGGATATTAAGAGTATGCTGTCATTTAATAATATAGCAATTCGTAAAACAAA
AGAGCCTGCCATCGAAAATAATCATTACTTAAATAAAAATAATAAAATAGTTGGGATTATAGATTATGGCATGGGAAATC
ATCAAAGTCTAATAAATGCATTACATGAATTAGGATATAGTTCAATTGTTAGTCATGTAGCAGAAGAACTAAACAACTGT
GAATTAATTGCATTGCCAGGTGTAGGTGCATTTGCCAAAGGGATGGATAATTTAAGAAGAATGGGTTTAGACCAATATCT
TATTAATGCATGCAACGAGGGCCGTCCATTAATAGGTATATGTCTTGGAATGCAATTGCTGTTTGAGTCAAGTGAAGAGT
ACGGCATCAATAAAGGATTGGGATTGCTAAACGGATGTGTGTCCAAAATGCAAGAACATTTCAGTAGTAAAGCCTTCTAT
CCGCTTCCACATATGGGTTGGAACTTAATAGAGTCTACTAAATTAGCCAAAGATTCTGGTCTGAGTGAAAACTCATTTTA
TCAATATTTTGTTCACAGCTATTCAGTGAAACCGAAGTCTGATGAGATTATTCTACATACTTGTGAATATGCAGAGAACT
CAATAGTCGCATCTGTTCTTTATGAGAATATATGTGGTCTTCAGTTTCACCCAGAAAGAAGTGGAGAAGTGGGGATTAAT
CTTCTAGATAAGATCCTGGAAAAATTGACTAGTGGCAAAGATGATCAAACCCAGAGCTTGTAA

Upstream 100 bases:

>100_bases
GATCACCTCATTTATGGACATGGGATCGTGACATCGGATGGAAGTTACGCCATCAGGTATCGAACAATAATATCGATCAA
AAAGAAACTGATCTTCTAGC

Downstream 100 bases:

>100_bases
GCATCGAACCTATTGTCATAGAAAATTAACATGTAATGGCTTTTCTTCATTAGGGTTGGCAGGATCAATAGATTTGATCC
CTTTTATATGATCATCAATA

Product: imidazoleglycerol-phosphate synthase

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MGRQSSKNTVKYMALSKRIIARLDVKGNKIIKGVRFEGLRVIGDPLDMARKYAKAGIDELLYIDAVASLYGRNSLIEVLK
STSSEVFIPITAGGGIRNLEDAKLLLSNGADKIAINTAALECPQLISELSNTLGSQCVVVSIQARASSSSSWDAMKESGR
EKSGIDVIKWIENVQLLGAGEILLTSVDQDGTCIKPDEILIQKVLPILKLPLIVSGGFSDPNQVYQALTNSRISAVAIGA
ALHYQKTNVSDIKSMLSFNNIAIRKTKEPAIENNHYLNKNNKIVGIIDYGMGNHQSLINALHELGYSSIVSHVAEELNNC
ELIALPGVGAFAKGMDNLRRMGLDQYLINACNEGRPLIGICLGMQLLFESSEEYGINKGLGLLNGCVSKMQEHFSSKAFY
PLPHMGWNLIESTKLAKDSGLSENSFYQYFVHSYSVKPKSDEIILHTCEYAENSIVASVLYENICGLQFHPERSGEVGIN
LLDKILEKLTSGKDDQTQSL

Sequences:

>Translated_500_residues
MGRQSSKNTVKYMALSKRIIARLDVKGNKIIKGVRFEGLRVIGDPLDMARKYAKAGIDELLYIDAVASLYGRNSLIEVLK
STSSEVFIPITAGGGIRNLEDAKLLLSNGADKIAINTAALECPQLISELSNTLGSQCVVVSIQARASSSSSWDAMKESGR
EKSGIDVIKWIENVQLLGAGEILLTSVDQDGTCIKPDEILIQKVLPILKLPLIVSGGFSDPNQVYQALTNSRISAVAIGA
ALHYQKTNVSDIKSMLSFNNIAIRKTKEPAIENNHYLNKNNKIVGIIDYGMGNHQSLINALHELGYSSIVSHVAEELNNC
ELIALPGVGAFAKGMDNLRRMGLDQYLINACNEGRPLIGICLGMQLLFESSEEYGINKGLGLLNGCVSKMQEHFSSKAFY
PLPHMGWNLIESTKLAKDSGLSENSFYQYFVHSYSVKPKSDEIILHTCEYAENSIVASVLYENICGLQFHPERSGEVGIN
LLDKILEKLTSGKDDQTQSL
>Mature_499_residues
GRQSSKNTVKYMALSKRIIARLDVKGNKIIKGVRFEGLRVIGDPLDMARKYAKAGIDELLYIDAVASLYGRNSLIEVLKS
TSSEVFIPITAGGGIRNLEDAKLLLSNGADKIAINTAALECPQLISELSNTLGSQCVVVSIQARASSSSSWDAMKESGRE
KSGIDVIKWIENVQLLGAGEILLTSVDQDGTCIKPDEILIQKVLPILKLPLIVSGGFSDPNQVYQALTNSRISAVAIGAA
LHYQKTNVSDIKSMLSFNNIAIRKTKEPAIENNHYLNKNNKIVGIIDYGMGNHQSLINALHELGYSSIVSHVAEELNNCE
LIALPGVGAFAKGMDNLRRMGLDQYLINACNEGRPLIGICLGMQLLFESSEEYGINKGLGLLNGCVSKMQEHFSSKAFYP
LPHMGWNLIESTKLAKDSGLSENSFYQYFVHSYSVKPKSDEIILHTCEYAENSIVASVLYENICGLQFHPERSGEVGINL
LDKILEKLTSGKDDQTQSL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=255, Percent_Identity=34.5098039215686, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=38.2352941176471, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=24.1706161137441, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=324, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 54647; Mature: 54515

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQSSKNTVKYMALSKRIIARLDVKGNKIIKGVRFEGLRVIGDPLDMARKYAKAGIDEL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCHHHHHHHHHHHCHHHH
LYIDAVASLYGRNSLIEVLKSTSSEVFIPITAGGGIRNLEDAKLLLSNGADKIAINTAAL
HHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHH
ECPQLISELSNTLGSQCVVVSIQARASSSSSWDAMKESGREKSGIDVIKWIENVQLLGAG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHEEEECC
EILLTSVDQDGTCIKPDEILIQKVLPILKLPLIVSGGFSDPNQVYQALTNSRISAVAIGA
CEEEEECCCCCCEECHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHEEEHH
ALHYQKTNVSDIKSMLSFNNIAIRKTKEPAIENNHYLNKNNKIVGIIDYGMGNHQSLINA
HHEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCEEEEEECCCCCHHHHHHH
LHELGYSSIVSHVAEELNNCELIALPGVGAFAKGMDNLRRMGLDQYLINACNEGRPLIGI
HHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHH
CLGMQLLFESSEEYGINKGLGLLNGCVSKMQEHFSSKAFYPLPHMGWNLIESTKLAKDSG
HHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
LSENSFYQYFVHSYSVKPKSDEIILHTCEYAENSIVASVLYENICGLQFHPERSGEVGIN
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCHH
LLDKILEKLTSGKDDQTQSL
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GRQSSKNTVKYMALSKRIIARLDVKGNKIIKGVRFEGLRVIGDPLDMARKYAKAGIDEL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCHHHHHHHHHHHCHHHH
LYIDAVASLYGRNSLIEVLKSTSSEVFIPITAGGGIRNLEDAKLLLSNGADKIAINTAAL
HHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHH
ECPQLISELSNTLGSQCVVVSIQARASSSSSWDAMKESGREKSGIDVIKWIENVQLLGAG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHEEEECC
EILLTSVDQDGTCIKPDEILIQKVLPILKLPLIVSGGFSDPNQVYQALTNSRISAVAIGA
CEEEEECCCCCCEECHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHEEEHH
ALHYQKTNVSDIKSMLSFNNIAIRKTKEPAIENNHYLNKNNKIVGIIDYGMGNHQSLINA
HHEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCEEEEEECCCCCHHHHHHH
LHELGYSSIVSHVAEELNNCELIALPGVGAFAKGMDNLRRMGLDQYLINACNEGRPLIGI
HHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHH
CLGMQLLFESSEEYGINKGLGLLNGCVSKMQEHFSSKAFYPLPHMGWNLIESTKLAKDSG
HHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
LSENSFYQYFVHSYSVKPKSDEIILHTCEYAENSIVASVLYENICGLQFHPERSGEVGIN
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCHH
LLDKILEKLTSGKDDQTQSL
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]