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The map label for this gene is acoA [H]

Identifier: 124021816

GI number: 124021816

Start: 103093

End: 103935

Strand: Direct

Name: acoA [H]

Synonym: P9303_01031

Alternate gene names: 124021816

Gene position: 103093-103935 (Clockwise)

Preceding gene: 124021815

Following gene: 124021817

Centisome position: 3.84

GC content: 35.82

Gene sequence:

>843_bases
ATGCGTGCATTTCAATTGGCAATAGCAGATGAATACCATAATCAAAAAATGAGATGTCCTGTTCATTTATCGATAGGACA
AGAATATTGGTTACCCTTGGTCAAGAAATTTTTTCAAAAAACAGATCGTTGTTTTTCAAGTCATCGATCACATAGTATGT
ATTTGGCGTTAGCTTGTGATCCCGAATCGCTCATAGCAGAACTACATGGATCTGCATTTGGATCATTGCAGGGATTAGGC
GGTAGTATGCATTTAAAAGAACTTGATTCAGGTCTGGAAGCTTCTATACCTATTGTTGGAAGTTCAATAGCTCTTGGTGT
GGGTAGTGCTTTATCAGCTAAACATTCTAAGAATGATGTACTAACAATTTCATATTTTGGAGATGGAGCTTGTGAAGAGG
GAATCTTTCATGAGTGTTTAAATCTAGCTGCCCTTTATAAGCTACCCATACTATTCATCTGTGAGAATAATAGCTATTCT
TGTAATACACATTACAGCAATCGACAAACAGCAAATGATATGACTAGATTTCCTCTGTCACATGGAATGACCAATTTTAA
AATTAACAATTTTGATCCATATCTTTTTATTGAATCCACAATGGAAAAATGCTTTTCCTTGGCTAGGGATAGACCTGTTT
TTCTTGAAATCTCCTCTTATCGGTTATATGAACACTGTGGACACCTGATCGACTTAGATCGAGGGGACAGGACTTACGAT
GAATACCTATATTATCAACGTATAGACCCTGTCAATCAAATGCTATCCATCTACCCGAACTTAAAAAACGATGAAACTAT
TTGGAAGAAATATTATATTGACTTGATTCGTAAGTATGAGTAG

Upstream 100 bases:

>100_bases
ATTCAATAGTAAATAATAAGATCGACCATATCAACTTTATTTAAACATGCAAGATAAATATCCAGATCAATACATGTATT
TATACAAGGATGCTGTTAAG

Downstream 100 bases:

>100_bases
TTTAATGAAGGATAAATTATCCATGAAACATGGTGAATTAATCAGAGAACAGACCTATGAATTTCTAAATAAATCGCCTA
ACCATATTCTCCTATGTGAA

Product: pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, subunit alpha

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRAFQLAIADEYHNQKMRCPVHLSIGQEYWLPLVKKFFQKTDRCFSSHRSHSMYLALACDPESLIAELHGSAFGSLQGLG
GSMHLKELDSGLEASIPIVGSSIALGVGSALSAKHSKNDVLTISYFGDGACEEGIFHECLNLAALYKLPILFICENNSYS
CNTHYSNRQTANDMTRFPLSHGMTNFKINNFDPYLFIESTMEKCFSLARDRPVFLEISSYRLYEHCGHLIDLDRGDRTYD
EYLYYQRIDPVNQMLSIYPNLKNDETIWKKYYIDLIRKYE

Sequences:

>Translated_280_residues
MRAFQLAIADEYHNQKMRCPVHLSIGQEYWLPLVKKFFQKTDRCFSSHRSHSMYLALACDPESLIAELHGSAFGSLQGLG
GSMHLKELDSGLEASIPIVGSSIALGVGSALSAKHSKNDVLTISYFGDGACEEGIFHECLNLAALYKLPILFICENNSYS
CNTHYSNRQTANDMTRFPLSHGMTNFKINNFDPYLFIESTMEKCFSLARDRPVFLEISSYRLYEHCGHLIDLDRGDRTYD
EYLYYQRIDPVNQMLSIYPNLKNDETIWKKYYIDLIRKYE
>Mature_280_residues
MRAFQLAIADEYHNQKMRCPVHLSIGQEYWLPLVKKFFQKTDRCFSSHRSHSMYLALACDPESLIAELHGSAFGSLQGLG
GSMHLKELDSGLEASIPIVGSSIALGVGSALSAKHSKNDVLTISYFGDGACEEGIFHECLNLAALYKLPILFICENNSYS
CNTHYSNRQTANDMTRFPLSHGMTNFKINNFDPYLFIESTMEKCFSLARDRPVFLEISSYRLYEHCGHLIDLDRGDRTYD
EYLYYQRIDPVNQMLSIYPNLKNDETIWKKYYIDLIRKYE

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=219, Percent_Identity=31.5068493150685, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI4505685, Length=219, Percent_Identity=29.6803652968037, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI291084742, Length=219, Percent_Identity=29.6803652968037, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI291084744, Length=227, Percent_Identity=29.5154185022026, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI258645172, Length=178, Percent_Identity=28.6516853932584, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI11386135, Length=178, Percent_Identity=27.5280898876405, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564172, Length=255, Percent_Identity=27.843137254902, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536047, Length=255, Percent_Identity=27.843137254902, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI86563357, Length=190, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI86563355, Length=190, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321026, Length=254, Percent_Identity=30.7086614173228, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24639746, Length=262, Percent_Identity=29.0076335877863, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639744, Length=262, Percent_Identity=29.0076335877863, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28571106, Length=262, Percent_Identity=29.0076335877863, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639740, Length=262, Percent_Identity=29.0076335877863, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24639748, Length=226, Percent_Identity=29.2035398230088, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355903, Length=186, Percent_Identity=26.3440860215054, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 32156; Mature: 32156

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFQLAIADEYHNQKMRCPVHLSIGQEYWLPLVKKFFQKTDRCFSSHRSHSMYLALACD
CCEEEEEEHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
PESLIAELHGSAFGSLQGLGGSMHLKELDSGLEASIPIVGSSIALGVGSALSAKHSKNDV
HHHHHHHHHCCHHHHHHCCCCCCCHHHHCCCCCCCCCEECCHHHHCCCCHHHCCCCCCCE
LTISYFGDGACEEGIFHECLNLAALYKLPILFICENNSYSCNTHYSNRQTANDMTRFPLS
EEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCHHHHHHCHHH
HGMTNFKINNFDPYLFIESTMEKCFSLARDRPVFLEISSYRLYEHCGHLIDLDRGDRTYD
CCCCEEEECCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCEEECCCCCCCHH
EYLYYQRIDPVNQMLSIYPNLKNDETIWKKYYIDLIRKYE
HHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRAFQLAIADEYHNQKMRCPVHLSIGQEYWLPLVKKFFQKTDRCFSSHRSHSMYLALACD
CCEEEEEEHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
PESLIAELHGSAFGSLQGLGGSMHLKELDSGLEASIPIVGSSIALGVGSALSAKHSKNDV
HHHHHHHHHCCHHHHHHCCCCCCCHHHHCCCCCCCCCEECCHHHHCCCCHHHCCCCCCCE
LTISYFGDGACEEGIFHECLNLAALYKLPILFICENNSYSCNTHYSNRQTANDMTRFPLS
EEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCCCCCHHHHHHCHHH
HGMTNFKINNFDPYLFIESTMEKCFSLARDRPVFLEISSYRLYEHCGHLIDLDRGDRTYD
CCCCEEEECCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCEEECCCCCCCHH
EYLYYQRIDPVNQMLSIYPNLKNDETIWKKYYIDLIRKYE
HHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]