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The map label for this gene is tig

Identifier: 124021785

GI number: 124021785

Start: 68501

End: 69940

Strand: Reverse

Name: tig

Synonym: P9303_00701

Alternate gene names: 124021785

Gene position: 69940-68501 (Counterclockwise)

Preceding gene: 124021788

Following gene: 124021784

Centisome position: 2.61

GC content: 52.71

Gene sequence:

>1440_bases
ATGAGTGCCACAGCACTACAGGTCAAAACCACGCAGAAACCCAATAGTCGGCTTGCTCTGGAAGTAGCGGTACCAGCAGA
GCGCTGCCAGGCCAACTATGAGGCCGCTGTCACCCGCTTAAGCCGCACCATCAATCTTCCAGGCTTCCGCAAGGGGAAGG
TTCCGCGTGCCGTTCTGCTGCAACAGATCGGCCTGGTAAGAATCCGTGCAACAGCCTTAGAAACGCTCGTCGATGCTGTC
TGGCGTGAAGTCCTCGAACAAGAATCAATCGAGCCACTTTGCGAACCCGAACTCAGTGGCGGCTTTGACGCTCTTCTGGA
AAGCTTTCAGCCCGGCGAAGCACTGACCCTCATCCTTGAGACAGACGTCACCCCAACCCCAAAACTTAAGGCCACCAAAG
GGCTCCAAGCCGAAGCAGAAGTGGTGACTTTCGATCCCAGCAAGGTCGATGAACTGATCGAACAATCCCGCAAACAACTA
GCAACCCTTGTACCCGTAGAAAGTCGCCCAGCAGCAATCGGCGATATCGCTGTGGTTAGTTTCAGCGGCACCTACGACGA
CGACGGCAGCGCAATCGAAGGCGGAAGTAGTGAGTCCATGGATGTCGATCTTGAAGATGGACAGATGATCCCCGGCTTCG
TGGAAGGGATTATCGGTATGAGCTTGGGCAGTGAGAAAACTGTTGATTGCCACTTCCCAGACGATTACTCGAAAGAGGAT
GCTCGCGGCCGTAAAGCCAGCTTTGTGATCAACCTCAAGGAGCTCAAAACCCGCGAGTTGCCGGATCTTGACGATGCCTT
CGCCCAGCAGTCCAGTGACAAAGCAACTCTGGAAGAACTTCGCAACGATCTAGAACAACGTCTTCAGGAAGACGCAAAGC
GTCGTGATCGCAGCAATCGGCACGACGCTTTGCTGGAAGCATTGACTGAGCAGCTGGAAGTAGATCTACCAAACACCCTT
GTGCAGCAAGAGATCCGCAACCTTGTTGAACAAACTGCCAGTCAATTCGCCCAACAGGGGATGGACGTGAAATCCATGTT
CACACCAGAGCTTGTGCGTTCCTTGATGGAATCATCACGCCCAGAAGCCGAAGAGCGCCTACGTCGCAGTCTTGCCCTCA
CTGCACTAGCTGAAAGCGAAGATCTCAAAATAGAAGAGAGTGAAATCAGCGCCAAGGTCAAGGAAGTAAGCCGCGAGCTT
TCTGGCGAACGCGATATTGACCCGGCAAGGTTGCGTCAAGCTGTTAGCGACGACTTACTCAAAGACAAATTGCTGGACTG
GCTCGAAGACAACAGCACCATTACCGAAAAGGTGCTTGAAAGCGAAGCAAAAACCAGTAAGCCCGCTGCAAAATCCAAAG
GCAGCAAAACTAAGTCCACAAAAACCAAGACCAACAAAGCCAAGACTGAAAAACCTGCCTCGGACAAAACCAAGTCCTGA

Upstream 100 bases:

>100_bases
CCCTACAGCCTCAATCAGGCAAGACGTTGAGACCACAATACGTGTTGAGAGATGAACTGACGCTTTCTTAGGGTGGTCTT
CTGCTCGCAGTCGTCCGTCC

Downstream 100 bases:

>100_bases
ATGGCACCAATAGATTGATAAAAGTGCTCCCCTAAAGCCCAAACGAGTGATCGATTCCTGCTGCTACCACCCCATCCACA
ATCGCTGGCCGGGCATTCGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSATALQVKTTQKPNSRLALEVAVPAERCQANYEAAVTRLSRTINLPGFRKGKVPRAVLLQQIGLVRIRATALETLVDAV
WREVLEQESIEPLCEPELSGGFDALLESFQPGEALTLILETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQL
ATLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSESMDVDLEDGQMIPGFVEGIIGMSLGSEKTVDCHFPDDYSKED
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTL
VQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSLALTALAESEDLKIEESEISAKVKEVSREL
SGERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKAKTEKPASDKTKS

Sequences:

>Translated_479_residues
MSATALQVKTTQKPNSRLALEVAVPAERCQANYEAAVTRLSRTINLPGFRKGKVPRAVLLQQIGLVRIRATALETLVDAV
WREVLEQESIEPLCEPELSGGFDALLESFQPGEALTLILETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQL
ATLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSESMDVDLEDGQMIPGFVEGIIGMSLGSEKTVDCHFPDDYSKED
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTL
VQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSLALTALAESEDLKIEESEISAKVKEVSREL
SGERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKAKTEKPASDKTKS
>Mature_478_residues
SATALQVKTTQKPNSRLALEVAVPAERCQANYEAAVTRLSRTINLPGFRKGKVPRAVLLQQIGLVRIRATALETLVDAVW
REVLEQESIEPLCEPELSGGFDALLESFQPGEALTLILETDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLA
TLVPVESRPAAIGDIAVVSFSGTYDDDGSAIEGGSSESMDVDLEDGQMIPGFVEGIIGMSLGSEKTVDCHFPDDYSKEDA
RGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNRHDALLEALTEQLEVDLPNTLV
QQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSRPEAEERLRRSLALTALAESEDLKIEESEISAKVKEVSRELS
GERDIDPARLRQAVSDDLLKDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKAKTEKPASDKTKS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=395, Percent_Identity=27.8481012658228, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PROM3 (A2C5R7)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016092.1
- ProteinModelPortal:   A2C5R7
- STRING:   A2C5R7
- GeneID:   4778281
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_00701
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 52852; Mature: 52721

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATALQVKTTQKPNSRLALEVAVPAERCQANYEAAVTRLSRTINLPGFRKGKVPRAVLL
CCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QQIGLVRIRATALETLVDAVWREVLEQESIEPLCEPELSGGFDALLESFQPGEALTLILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEE
TDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLATLVPVESRPAAIGDIAVVS
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FSGTYDDDGSAIEGGSSESMDVDLEDGQMIPGFVEGIIGMSLGSEKTVDCHFPDDYSKED
ECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHH
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNR
CCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HDALLEALTEQLEVDLPNTLVQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCC
PEAEERLRRSLALTALAESEDLKIEESEISAKVKEVSRELSGERDIDPARLRQAVSDDLL
CCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKAKTEKPASDKTKS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SATALQVKTTQKPNSRLALEVAVPAERCQANYEAAVTRLSRTINLPGFRKGKVPRAVLL
CCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QQIGLVRIRATALETLVDAVWREVLEQESIEPLCEPELSGGFDALLESFQPGEALTLILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEE
TDVTPTPKLKATKGLQAEAEVVTFDPSKVDELIEQSRKQLATLVPVESRPAAIGDIAVVS
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FSGTYDDDGSAIEGGSSESMDVDLEDGQMIPGFVEGIIGMSLGSEKTVDCHFPDDYSKED
ECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCHH
ARGRKASFVINLKELKTRELPDLDDAFAQQSSDKATLEELRNDLEQRLQEDAKRRDRSNR
CCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HDALLEALTEQLEVDLPNTLVQQEIRNLVEQTASQFAQQGMDVKSMFTPELVRSLMESSR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCC
PEAEERLRRSLALTALAESEDLKIEESEISAKVKEVSRELSGERDIDPARLRQAVSDDLL
CCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KDKLLDWLEDNSTITEKVLESEAKTSKPAAKSKGSKTKSTKTKTNKAKTEKPASDKTKS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA