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The map label for this gene is gcvH [H]

Identifier: 123969410

GI number: 123969410

Start: 1614242

End: 1614631

Strand: Reverse

Name: gcvH [H]

Synonym: A9601_18781

Alternate gene names: 123969410

Gene position: 1614631-1614242 (Counterclockwise)

Preceding gene: 123969411

Following gene: 123969409

Centisome position: 96.69

GC content: 35.64

Gene sequence:

>390_bases
ATGTCTTACAAGTTTCCAGACAACCTCAACTATGCTGATACTCATGAATATGTCTTGGAAGAAAATGGATTATTAAAAAT
TGGAGTTAGTGAATTCGCTATAGATCAATTAGGAGATATTGTTTTTGTTGAATTAGCTGATGAAGGTGCGACTTTAGAGA
AAGGCGAGACTTTTGGAACCATAGAATCAGTTAAGGCCGTTGAGGAAGTCTATTTGCCTTTTTCAGGGGAAATAGTATCT
GTAAATGAAAGTGTTATTGAGAACCCTGAGCTTTTACAGAATGATCCGATTGGAGAAGGTTGGTTAGTCATTTTGAAACC
AGAATCAAAAGCATCAATTGCTGATTTGATGACCTCTGAGGAATATCAATCAAAGGTTGTACCAAAATAA

Upstream 100 bases:

>100_bases
ACATAGCTCACATCAAAATTGCATTAATTCGATTATTATCTGAACTATTAGAGGAAAAATTAATTTCAAAGGATTCTCTA
CTTCCTTTATCTACTTAATC

Downstream 100 bases:

>100_bases
GGCAAACTTTTTAAAAAGAGCTATTTTAAAGAAAAATATTTTAATATGACATCTAAATTTGGGTCTGATTTGTTTGTAGA
TAGGCATCTTGGGTTAGGAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIENPELLQNDPIGEGWLVILKPESKASIADLMTSEEYQSKVVPK

Sequences:

>Translated_129_residues
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIENPELLQNDPIGEGWLVILKPESKASIADLMTSEEYQSKVVPK
>Mature_128_residues
SYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVSV
NESVIENPELLQNDPIGEGWLVILKPESKASIADLMTSEEYQSKVVPK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=38.9380530973451, Blast_Score=98, Evalue=2e-21,
Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=38.9380530973451, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=36.6666666666667, Blast_Score=93, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=41.9642857142857, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=35.8333333333333, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319272, Length=130, Percent_Identity=33.8461538461538, Blast_Score=90, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=34.4827586206897, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14257; Mature: 14126

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGT
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIENPELLQNDPIGEGWLVILKPESKASIADLMTSE
HHHHHHHHHHCCCCCCCEEECCHHHHCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYQSKVVPK
HHHHCCCCC
>Mature Secondary Structure 
SYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGT
CCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIENPELLQNDPIGEGWLVILKPESKASIADLMTSE
HHHHHHHHHHCCCCCCCEEECCHHHHCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYQSKVVPK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA