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The map label for this gene is prfA [H]

Identifier: 123969269

GI number: 123969269

Start: 1482389

End: 1483483

Strand: Reverse

Name: prfA [H]

Synonym: A9601_17371

Alternate gene names: 123969269

Gene position: 1483483-1482389 (Counterclockwise)

Preceding gene: 123969270

Following gene: 123969267

Centisome position: 88.84

GC content: 36.26

Gene sequence:

>1095_bases
TTGGAATACTCAACATTAATTGCAAGGTTGAAAACTGCTTCAGAAAGTTTTGAAAATTTGGAGGTTCAGCTTGCAGATCC
TGATATAGCTAATGATCCCAAAAAATTAGAATCAATAGCAAGAGAAAGGTCAAAATTGGAACCATTGGTTATTGATTTTA
ATAAGTTGCTTGATACTGATAAAGAAATCGAAGATTCAAAAAATCTACTAAAAGAGAATAGAAATGATAAAGAAATGGAA
TCTTTGATAAACGAGGAGTTAATAAATCTAGAGGAAATTAAGAATGAACTGATTCAAAAAACCACTATCGCCTTATTACC
AAAAGATCCTAGAGATGAAAGAAGTGTAATGTTAGAAATCAGAGCTGGTGCTGGAGGTAATGAGGCTTGTATTTGGGCTG
GTGATTTAGCAAGAATGTATGAGAGATATGGACAAAAAATTGGATGGTCTGTAAAACCTGTTAGTGCTTCCGAGTCCGAT
ATGGGTGGATTTAAGGAGTTAGTTATATCCGTTAAGGGTGATTCTGTATACAGTCAGCTTAAATTTGAGGCTGGTGTACA
TAGAGTCCAAAGAGTTCCAGCTACTGAATCCCAAGGTAGAGTTCACACCTCTACAGCTACAGTGGCAGTTATGCCTGAGG
CTGATCCTGTTGAGGTTAAGATTGATCCGATTGATCTAGAGGTTGGTACCGCGAGATCAGGAGGAGCAGGGGGACAAAAT
GTTAATAAGGTAGAGACGGCTATTGATCTTCTCCACAAGCCTACAGGAATAAGAGTTTTTTGTACTCAGGAGAGATCACA
ATTGCAAAATCGTGAACGAGCGATGGAAATTTTAAGAGCTAAATTATATGAAATTCAATTAAAAGAAGCTAATGCAAAAG
AGAGATCACAAAGGCTTTCTCAGGTTGGGACAGGCGATAGGAGTGAAAAAATTAGAACTTATAATTTTAAAGATAATAGG
ACAACTGATCATAGATTAGGTTCCAATTTTTCACTTGAGCCAATTCTTGCTGGTCAATTAGATGAAGTAATTGATGCATG
CATAGCGCAAGAACAAAAAAGAATGATGGAAGATTTTAATAACGAAGTGAATTAA

Upstream 100 bases:

>100_bases
AAATATGGAATGGGTTCAGCTAATTCAGCCACATCAAAAGAGCAAAAAGAAGAAAAAGATTCTAAAAAATAGATTTTCCA
AAAATTAAGCAAAATTTCAA

Downstream 100 bases:

>100_bases
GTTTTTATTTTTATTAGATTGCAACCCCTATTACGTATTTACTCCATTCTTTATGTCTGCCAGAGTCAATTTGTCTTGTA
GCCTCAAAATTGAGATTACT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEEIKNELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPIDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNEVN

Sequences:

>Translated_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEEIKNELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPIDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNEVN
>Mature_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEEIKNELIQKTTIALLPKDPRDERSVMLEIRAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPIDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNEVN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=40.1315789473684, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=34.8441926345609, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=36.3636363636364, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=42.5714285714286, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=34.3195266272189, Blast_Score=201, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=307, Percent_Identity=36.1563517915309, Blast_Score=184, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=253, Percent_Identity=43.8735177865613, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI19921226, Length=311, Percent_Identity=38.2636655948553, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40918; Mature: 40918

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELINLEEIKNELIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPIDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFN
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC
NEVN
CCCC
>Mature Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELINLEEIKNELIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGNEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPIDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFN
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC
NEVN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA