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The map label for this gene is purB [H]

Identifier: 123969199

GI number: 123969199

Start: 1407842

End: 1409137

Strand: Direct

Name: purB [H]

Synonym: A9601_16671

Alternate gene names: 123969199

Gene position: 1407842-1409137 (Clockwise)

Preceding gene: 123969197

Following gene: 123969200

Centisome position: 84.31

GC content: 35.34

Gene sequence:

>1296_bases
GTGATCGAGCGTTACACATTACCCAAAATGGGGAAAATCTGGACTGAAAGCGCAAAATTCCAGAGTTGGCTTAAGGTTGA
AATAGCTGCATGTGAAGCAAATTTTTCCCTCGGGAAAATCCCTGAGAATGCCATGAAAGAGATACGTTCAAATGCAAAGT
TTGATGAATCTAGAATCACAGAAATTGAGAAAGAAGTTAAACATGATGTCATAGCATTTCTTACAAGCGTTAATGAATTT
GTAGGAGATTCAGGAAGATACATACATGTTGGTATGACCAGTAGTGATGTACTTGATACTGGCTTATCTCTTCAGTTAAA
AGATTCTTGCGAATTGCTATTAGAAGAAATTGAGAACCTAGAAAATGAGGTCAGATTATTAGCAAGGAAGCATAAAAATA
CATTAATGATTGGCAGATCTCATGCAATTCATGGGGAGCCAATTTCCTTCGGTTTTAAACTTGCTGGATGGTTAGCAGAA
ATAATAAGGAACAAAAAAAGATTGTTAACTCTGAAAGATTCTGTAGCAATTGGACAAATAAGTGGTGCAATGGGAACTTA
CGCTAATACAAATCCTAAAGTAGAACAAATAACTTGTGATTTACTCGGCTTAAAACCTGATACAGCAAGTACGCAGGTTA
TATCGAGAGACAGACATGCAGAATATGTGCAAACTATTGCACTAGTTGGAGCTTCTTTAGATAGATTCGCGACTGAAATA
AGAAATTTACAAAGAACTGATGTTTTAGAAGTTGAGGAGGGCTTTACAAAAGGGCAAAAAGGAAGTTCTGCCATGCCTCA
TAAAAGAAATCCTATTCGGAGTGAAAGGGTAAGTGGTTTAGCAAGAATTTTGAGGAGTTATGTCTTAACCGCACTTGAAA
ATGTTCCACTTTGGCACGAAAGAGATATAAGCCATAGTTCAAATGAACGTATCATGCTACCTGACGTATCAATCTGCTTG
CATTTTATGCTCAGGGAAATGCAAGATATAGTAAGCAATTTAGAAGTTTATCCAAAAAATATGCTCAAAAATTTAAATAT
ATATGGTGGTGTAATCTTTAGTCAGAAAGTTTTACTTTTGCTTGTAGAAAAGGGATTGTCTAGAGAAAAAGCTTATAGCT
TAGTGCAAAAAAATGCGCATCAGGCCTGGAACACGCAGAATGGGAATTTCAAACAAAATATAGAGGGCGATAATGAAATT
ATGGATTATATTGATCAAAGTGACTTAGAAGAATGTTTTAATCCTTCAATTCATCTTAATAATTTAAGTGTAATATGGGA
GAAGTTAGGTATCTAG

Upstream 100 bases:

>100_bases
AATAATCAGTCAAATATGTTCAATGGCAAGTACATCTTTAAAGGTATAGGGCAATAATTCGATTTTGTTATAAAGTTTAA
ATTGATAATAAATAAAAATT

Downstream 100 bases:

>100_bases
GATTACTGAAAACCTTATCTAAGTTAAACCAGTGTTTTCAGAGGAATAATGACCAAAAATTTTAGGATTGAAAAAGATAG
TATGGGAACAATAGAGGTTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPKMGKIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLLEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKDSVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQNGNFKQNIEGDNEI
MDYIDQSDLEECFNPSIHLNNLSVIWEKLGI

Sequences:

>Translated_431_residues
MIERYTLPKMGKIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLLEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKDSVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQNGNFKQNIEGDNEI
MDYIDQSDLEECFNPSIHLNNLSVIWEKLGI
>Mature_431_residues
MIERYTLPKMGKIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLLEEIENLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKDSVAIGQISGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMQDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQNGNFKQNIEGDNEI
MDYIDQSDLEECFNPSIHLNNLSVIWEKLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=440, Percent_Identity=27.5, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1787376, Length=326, Percent_Identity=26.3803680981595, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790398, Length=253, Percent_Identity=25.296442687747, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=392, Percent_Identity=28.8265306122449, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564234, Length=334, Percent_Identity=29.3413173652695, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323391, Length=443, Percent_Identity=25.7336343115124, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=25.9162303664921, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48782; Mature: 48782

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPKMGKIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIT
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLLEEIENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKDSVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCEEHHHH
SGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMQDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTQNGNFKQNIEGDNEIMDYIDQSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPKMGKIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIT
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDTGLSLQLKDSCELLLEEIENL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKDSVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHEEEEECCEEHHHH
SGAMGTYANTNPKVEQITCDLLGLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMQDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTQNGNFKQNIEGDNEIMDYIDQSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]