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The map label for this gene is truB

Identifier: 123969080

GI number: 123969080

Start: 1327075

End: 1327998

Strand: Direct

Name: truB

Synonym: A9601_15481

Alternate gene names: 123969080

Gene position: 1327075-1327998 (Clockwise)

Preceding gene: 123969078

Following gene: 123969082

Centisome position: 79.47

GC content: 30.63

Gene sequence:

>924_bases
ATGGAAACTAAAGATGGATTCTTAGTAATTAATAAAGATAAAGGCTGTACCTCTCATGATTGTGTTAAACAAATAAGGAA
GTTACTAAATACAAAAAAGGTTGGGCACACAGGAACTCTTGACCCAGAAGTTATAGGAACATTACCAATCGCTATAGGCA
ATGCAACAAGATTTATTCAATATCTTCCCCAGGGTAAAACCTATATAGGAGAAATTAAATTAGGGATAAGAACTAGCACT
GATGATATTCAAGGAGAAATAATTAGTCAAAAAAGTTGGCCTAAAATCAGCTATAAACAATTAGATCAATACTTCAATAG
ATTTAGAGGAATTATTAAACAAATTCCGCCGAAAGTTTCTAGTGTGCACGTCAATGGAGAGAGAGCTTATAAAAAATCTT
TCAGGAATGAAATTTTTGAATTAGCACCAAGAGAAGTAAAAATAGACGAACTCATTTTAATGAACTGGGACCAAATTAAC
GGAATCATAGAAATAAAAATCAAATGTTCAGCTGGCACATATATAAGATCAATCGCGAGAGATATAGGAGAAACTCTTAA
TTCCGCAGGTTGCCTTCTTCAACTAAAAAGAATTTCAGCTTGTGGCTTTGATGAACAAAACTCGATAAAAATATCTGATA
TTGAAAAAGAAAAAGGAAAAAAAAACTCGAAAAATTTTATTATTCCAACAATTTCTGCTCTTAGTCACATTTCAACATTT
GTCTTAAGTAATGAAGAACAAATCAATTTTTGGCAAACAGGAAGAGAAATTAAAGTTGATATTAATTACTTCCGGGAAAG
CAAATCATTTGACTATAAAAAACCTATAAAAGTAATAGATAACAAACAAACACTTCTTGGGATAGGCTTCTTAAATAAAG
AGCAATCTAATATAAATCCAAAATTAGTCCTTAATGCTAAATAA

Upstream 100 bases:

>100_bases
TAAAATGAAATTATGGTTTTTCAATTTCTTTCTCTAAATTTCCAATACCAAAATCATAGAATGGTTATAAATCTTTCTGA
AAGTATTCTTAATATTAAAA

Downstream 100 bases:

>100_bases
CTTCTATAGGTATTCTTTTCTAAAAGGTTTAATCTGAACACCCCTATAAAAATGCTTTAGTATTCTTTCAGCTTTTTGGC
CTCTAGACGCCAGATATTTT

Product: putative tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGTLPIAIGNATRFIQYLPQGKTYIGEIKLGIRTST
DDIQGEIISQKSWPKISYKQLDQYFNRFRGIIKQIPPKVSSVHVNGERAYKKSFRNEIFELAPREVKIDELILMNWDQIN
GIIEIKIKCSAGTYIRSIARDIGETLNSAGCLLQLKRISACGFDEQNSIKISDIEKEKGKKNSKNFIIPTISALSHISTF
VLSNEEQINFWQTGREIKVDINYFRESKSFDYKKPIKVIDNKQTLLGIGFLNKEQSNINPKLVLNAK

Sequences:

>Translated_307_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGTLPIAIGNATRFIQYLPQGKTYIGEIKLGIRTST
DDIQGEIISQKSWPKISYKQLDQYFNRFRGIIKQIPPKVSSVHVNGERAYKKSFRNEIFELAPREVKIDELILMNWDQIN
GIIEIKIKCSAGTYIRSIARDIGETLNSAGCLLQLKRISACGFDEQNSIKISDIEKEKGKKNSKNFIIPTISALSHISTF
VLSNEEQINFWQTGREIKVDINYFRESKSFDYKKPIKVIDNKQTLLGIGFLNKEQSNINPKLVLNAK
>Mature_307_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGTLPIAIGNATRFIQYLPQGKTYIGEIKLGIRTST
DDIQGEIISQKSWPKISYKQLDQYFNRFRGIIKQIPPKVSSVHVNGERAYKKSFRNEIFELAPREVKIDELILMNWDQIN
GIIEIKIKCSAGTYIRSIARDIGETLNSAGCLLQLKRISACGFDEQNSIKISDIEKEKGKKNSKNFIIPTISALSHISTF
VLSNEEQINFWQTGREIKVDINYFRESKSFDYKKPIKVIDNKQTLLGIGFLNKEQSNINPKLVLNAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=212, Percent_Identity=31.1320754716981, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4503337, Length=208, Percent_Identity=27.4038461538462, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI215599015, Length=208, Percent_Identity=27.4038461538462, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI2367200, Length=213, Percent_Identity=31.4553990610329, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553978, Length=211, Percent_Identity=27.0142180094787, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323204, Length=211, Percent_Identity=26.5402843601896, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROMS (A2BSS0)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001009938.1
- STRING:   A2BSS0
- GeneID:   4718274
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_15481
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2516628
- BioCyc:   PMAR146891:A9601_15481-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34868; Mature: 34868

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGTLPIAIGNATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCHHHHHH
YLPQGKTYIGEIKLGIRTSTDDIQGEIISQKSWPKISYKQLDQYFNRFRGIIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKSFRNEIFELAPREVKIDELILMNWDQINGIIEIKIKCSAGTYIRSIAR
EEEECCHHHHHHHHHHHHHHHCCCCEEECCEEEECHHHCCCEEEEEEEECCCHHHHHHHH
DIGETLNSAGCLLQLKRISACGFDEQNSIKISDIEKEKGKKNSKNFIIPTISALSHISTF
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECHHHCCCCCCCCEEEEHHHHHHHHHHH
VLSNEEQINFWQTGREIKVDINYFRESKSFDYKKPIKVIDNKQTLLGIGFLNKEQSNINP
HCCCCCCEEHHHCCCEEEEEEEEEECCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCC
KLVLNAK
EEEEECC
>Mature Secondary Structure
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGTLPIAIGNATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCHHHHHH
YLPQGKTYIGEIKLGIRTSTDDIQGEIISQKSWPKISYKQLDQYFNRFRGIIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKSFRNEIFELAPREVKIDELILMNWDQINGIIEIKIKCSAGTYIRSIAR
EEEECCHHHHHHHHHHHHHHHCCCCEEECCEEEECHHHCCCEEEEEEEECCCHHHHHHHH
DIGETLNSAGCLLQLKRISACGFDEQNSIKISDIEKEKGKKNSKNFIIPTISALSHISTF
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECHHHCCCCCCCCEEEEHHHHHHHHHHH
VLSNEEQINFWQTGREIKVDINYFRESKSFDYKKPIKVIDNKQTLLGIGFLNKEQSNINP
HCCCCCCEEHHHCCCEEEEEEEEEECCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCC
KLVLNAK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA