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The map label for this gene is glgX [H]

Identifier: 123969054

GI number: 123969054

Start: 1302144

End: 1304177

Strand: Direct

Name: glgX [H]

Synonym: A9601_15221

Alternate gene names: 123969054

Gene position: 1302144-1304177 (Clockwise)

Preceding gene: 123969053

Following gene: 123969055

Centisome position: 77.98

GC content: 31.81

Gene sequence:

>2034_bases
GTGACTCATATCAATAAAGGTAAACCATTTCCCCTAGGAAGTTCTCTAACTTCACAAGGGGTTAATTTTTCCCTAGTAGC
CACAAATGCAGAATATGTAGAAATCTTATTGTTTGAGAAAGAGGGCTCTATTTCCCCAAAAAGTATATTGAAACTAGATC
AGAATCATAATACAGGTCCCTACTGGCATGCAGAGGTAAAGAATCTAAATGAAGGTTGTATTTATGCTTTCAGAGTAAAA
CAAAAAAATAATGCGATCAACAATAACTATGAAAAAAAAGTATTACTCGATCCATGTTCAAGGGGTATAACTGGGTGGGA
AAGTTATAAAAGAGAAAATGCATTAAAAACACAAGAAAATACTAATTCTTGTCTTAAAAGCGTTGTTTGCGATAGAAAAT
TATTTAATTTTAAGGATTTTCCAAGACCGAAACATTCTTGGGAAGAAACAATAATTTATGAACTCCATATCAAATCCTTC
ACTGAATCAAATGATAAAACCGAAAGTTGTTTCAAGAAATTTTTAAAAAAAATTCCATATCTCAAAGAACTGGGAATTAC
CTCTATAGAATTACTTCCAATTTTTTGTTTTGATCCAACTGATGCACCAAGTGGTTTAGAGAATTTTTGGGGTTATAGTC
CAATTAATTGGTTTACTCCGCATTTTGAATACGTTTCGAATGAATCAGCTGAAAAGAATAGAGAGGAATTTAGAAAATTA
GTAGAGGAGTGTCATAAGTCAGGCATTGAAGTTATTTTAGATGTCGTATACAATCACACTTCTGAAGGAGATTACAAAGG
GCCTGCGATATGTTGGAAAGGTATAGATGAAAATCTTTATTACTTTATTGGGAAAGATAAAAATTATCAGGACGTATCTG
GTTGTGGGAATACTATTGCAGCAAACAGAGGATTAGTTAGAAAACTAATTATTGAATCATTAAAGTGTTGGGCGAGTGAA
TTTGGAGTAGATGGTTTTAGATTTGATTTAGGTATTGCCTTATCAAGAGGTGAAAATCTTTCGCCACTCGATAATCCTCC
AATTTTTGAAGATATAGAATGTGAGCCAGAACTTGTTGATATAAAGTTTATAAGTGAGCCATGGGATTGTGGTGGGTTAT
ATAAATTAGGTGATTTCCCATCTAAGAATACTTTCACTTGGAATGGTCATTTTAGAGATGATTTGCGTAGATTTTGGAAA
GGGGATAAAGATACAGCTTGGAATATGAGCGATAAAATCAAAGGTACTCCATCGATTTATAAAGAAGATACTATTTTCCC
AAAATCAATAAACTTTATTACTTCACATGATGGATTTACTCTAAAAGATTTAGTAACTTTCAATAGAAAACATAATTTTG
CCAATAGAGAACAAAACAGAGATGGTGATAACCATAACAATTCTTGGAATCATGGTACGGAGGGACCAACTACAAATTTA
TTAATTAATGATTTAAGAAAAAGACAACAAAAAAATCTTATTCTTAGTTTACTTATCTCTAAAGGTGTTCCAATGATACT
AATGGGTGATGAGATAGGAAGATCACAAGGCGGTAATAATAATTCTTGGTGCCAAAATAATTTACTGGGCTGGATGAATT
GGGAACATGGTCAACAAGATTTGGAATTATTAGAATATTTTAAATACGTTATAAAAATCCGAAAAAAACTAATACACATT
TTTAATCCATCATTCTTTCCTAATAATCAAACCAATGAAAATATTCCAACATATCATTGGCATGGAACAAAGTTAGATAG
CCCAGATTGGAGTAGTTGGTCACACACAGTTGCATTTAGCATTAACGAAGGCAATACTAATCCGCTGGTCTGGATAGGTT
TAAATGCATATTCAAAAAGTATCGATTTCCCTTTGCCGAAATGTAAATATAATTGGCTAAAAGTTGTTGACACTAGCATG
TCCGAGATTTTTGAACCCTTAACTATTAATCAAAAATCTGTTTCAATAAAGAGTAGAAGCTCTTTATTAATTATCTCAGA
AGAAGTATTTTTGGAAAAAAATAATATATTCTAA

Upstream 100 bases:

>100_bases
CTTATATTTTAGTTTTTTTCTCAATTTTGTATCTATTATTCTAATATCAGTAATTTGAATAAATTATCTCAATATTTAAT
TAATCAATGATAAAGAGAAC

Downstream 100 bases:

>100_bases
AAGCGGGCGGCGGGAATCGAACCCGCATCTTCAGCTTGGAAGGCTGAGGTTTTACCACTAAACCACGCCCGCATTAAGTA
ATAGAATTACCATTGCAATA

Product: putative isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MTHINKGKPFPLGSSLTSQGVNFSLVATNAEYVEILLFEKEGSISPKSILKLDQNHNTGPYWHAEVKNLNEGCIYAFRVK
QKNNAINNNYEKKVLLDPCSRGITGWESYKRENALKTQENTNSCLKSVVCDRKLFNFKDFPRPKHSWEETIIYELHIKSF
TESNDKTESCFKKFLKKIPYLKELGITSIELLPIFCFDPTDAPSGLENFWGYSPINWFTPHFEYVSNESAEKNREEFRKL
VEECHKSGIEVILDVVYNHTSEGDYKGPAICWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
FGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELVDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGTPSIYKEDTIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTTNL
LINDLRKRQQKNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGQQDLELLEYFKYVIKIRKKLIHI
FNPSFFPNNQTNENIPTYHWHGTKLDSPDWSSWSHTVAFSINEGNTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVVDTSM
SEIFEPLTINQKSVSIKSRSSLLIISEEVFLEKNNIF

Sequences:

>Translated_677_residues
MTHINKGKPFPLGSSLTSQGVNFSLVATNAEYVEILLFEKEGSISPKSILKLDQNHNTGPYWHAEVKNLNEGCIYAFRVK
QKNNAINNNYEKKVLLDPCSRGITGWESYKRENALKTQENTNSCLKSVVCDRKLFNFKDFPRPKHSWEETIIYELHIKSF
TESNDKTESCFKKFLKKIPYLKELGITSIELLPIFCFDPTDAPSGLENFWGYSPINWFTPHFEYVSNESAEKNREEFRKL
VEECHKSGIEVILDVVYNHTSEGDYKGPAICWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
FGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELVDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGTPSIYKEDTIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTTNL
LINDLRKRQQKNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGQQDLELLEYFKYVIKIRKKLIHI
FNPSFFPNNQTNENIPTYHWHGTKLDSPDWSSWSHTVAFSINEGNTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVVDTSM
SEIFEPLTINQKSVSIKSRSSLLIISEEVFLEKNNIF
>Mature_676_residues
THINKGKPFPLGSSLTSQGVNFSLVATNAEYVEILLFEKEGSISPKSILKLDQNHNTGPYWHAEVKNLNEGCIYAFRVKQ
KNNAINNNYEKKVLLDPCSRGITGWESYKRENALKTQENTNSCLKSVVCDRKLFNFKDFPRPKHSWEETIIYELHIKSFT
ESNDKTESCFKKFLKKIPYLKELGITSIELLPIFCFDPTDAPSGLENFWGYSPINWFTPHFEYVSNESAEKNREEFRKLV
EECHKSGIEVILDVVYNHTSEGDYKGPAICWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASEF
GVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELVDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWKG
DKDTAWNMSDKIKGTPSIYKEDTIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTTNLL
INDLRKRQQKNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGQQDLELLEYFKYVIKIRKKLIHIF
NPSFFPNNQTNENIPTYHWHGTKLDSPDWSSWSHTVAFSINEGNTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVVDTSMS
EIFEPLTINQKSVSIKSRSSLLIISEEVFLEKNNIF

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=577, Percent_Identity=37.261698440208, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322245, Length=237, Percent_Identity=27.0042194092827, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78040; Mature: 77909

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHINKGKPFPLGSSLTSQGVNFSLVATNAEYVEILLFEKEGSISPKSILKLDQNHNTGP
CCCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHEECCCCCCCC
YWHAEVKNLNEGCIYAFRVKQKNNAINNNYEKKVLLDPCSRGITGWESYKRENALKTQEN
EEEHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEECHHHCCCCCHHHHHHHHCCCCCCH
TNSCLKSVVCDRKLFNFKDFPRPKHSWEETIIYELHIKSFTESNDKTESCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCHHHHHHHHHHHHCCC
LKELGITSIELLPIFCFDPTDAPSGLENFWGYSPINWFTPHFEYVSNESAEKNREEFRKL
HHHCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
VEECHKSGIEVILDVVYNHTSEGDYKGPAICWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASEFGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELVD
HCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGTPSIY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDTIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTTNL
CCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQKNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGQQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCHHH
LELLEYFKYVIKIRKKLIHIFNPSFFPNNQTNENIPTYHWHGTKLDSPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INEGNTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVVDTSMSEIFEPLTINQKSVSIKSRS
EECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHEEHHHHHHHHCCCCCCCCEEEEECCC
SLLIISEEVFLEKNNIF
CEEEEEHHHEEECCCCC
>Mature Secondary Structure 
THINKGKPFPLGSSLTSQGVNFSLVATNAEYVEILLFEKEGSISPKSILKLDQNHNTGP
CCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHEECCCCCCCC
YWHAEVKNLNEGCIYAFRVKQKNNAINNNYEKKVLLDPCSRGITGWESYKRENALKTQEN
EEEHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEECHHHCCCCCHHHHHHHHCCCCCCH
TNSCLKSVVCDRKLFNFKDFPRPKHSWEETIIYELHIKSFTESNDKTESCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCHHHHHHHHHHHHCCC
LKELGITSIELLPIFCFDPTDAPSGLENFWGYSPINWFTPHFEYVSNESAEKNREEFRKL
HHHCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
VEECHKSGIEVILDVVYNHTSEGDYKGPAICWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASEFGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELVD
HCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGTPSIY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDTIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGTEGPTTNL
CCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQKNLILSLLISKGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGQQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCHHH
LELLEYFKYVIKIRKKLIHIFNPSFFPNNQTNENIPTYHWHGTKLDSPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INEGNTNPLVWIGLNAYSKSIDFPLPKCKYNWLKVVDTSMSEIFEPLTINQKSVSIKSRS
EECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHEEHHHHHHHHCCCCCCCCEEEEECCC
SLLIISEEVFLEKNNIF
CEEEEEHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]