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The map label for this gene is leuA [H]

Identifier: 123968704

GI number: 123968704

Start: 985583

End: 987223

Strand: Direct

Name: leuA [H]

Synonym: A9601_11711

Alternate gene names: 123968704

Gene position: 985583-987223 (Clockwise)

Preceding gene: 123968702

Following gene: 123968705

Centisome position: 59.02

GC content: 36.81

Gene sequence:

>1641_bases
ATGTCAAAAGATCCTGGAAGAATTTTGATATTTGATACAACTCTTCGAGATGGAGAGCAATCTCCTGGTGCCAGTTTAAA
TCTTGAAGAAAAACTTGCTATCGCCCATCAATTGGCAAGATTAGGGGTGGATGTTATCGAGGCTGGATTCCCTTTCGCAA
GTCCTGGAGATTTTAAAGCTGTTAATAAAATTGCCAATGCCGTAGGGAAAGAAAATGGCCCTATAATATGTGGTTTGGCC
AGAGCCTCAAAAGGGGATATAAAAGCATGTTATGAAGCAGTAAGTCCAGCGCCAAAAAAAAGAATACATACTTTTATTGC
GACAAGTGATATTCATCTTAAACATAAACTTAAAAAATCCAGAAAAGATGTTCTGCAAATAGTCCCAGAAATGGTTAACT
ATGCAAAATCATTGGTCGATGATATTGAGTTTTCTTGTGAAGACGCCTCAAGGAGTGATCCTGATTTTTTATATGAAGTG
ATCCAACTAGCAATTTCAGCAGGGGCGACAACGATAAATATACCTGATACTGTTGGATTCACAACCCCAAGTGAATTTGG
CAACCTAATTGCAGATATAAATAAAAATGTTCCAAATATTAATGAGGCAGTAATCTCGGTTCATGGTCATAATGATTTAG
GTTTAGCAGTAGCTAATTTTCTTGAGGCAGTAAAAAATGGAGCAAGACAATTGGAATGTACCATTAATGGAATTGGAGAA
AGAGCCGGGAATGCCTCCCTTGAGGAATTAGTAATGGCACTGCACGTTAGAAAAAGTTTTTTTAATAGTTTTTTCAAAAG
AAATCCAGATTCACCAACTCCTCTTACAGCAATAAGAACAGAAGAAATAACAAAAACCTCTAGACTTGTTTCCAACCTAA
CTGGAATGACAGTACAACCTAATAAAGCAATTGTGGGAGCTAACGCTTTTGCACACGAGTCAGGCATTCATCAGGATGGG
GTTTTAAAAAATAGACTAACTTACGAAATTATCGATGCAAAAACTGTTGGTTTGAGTGACAACAAAATATCTTTGGGGAA
GCTTAGTGGAAGAAGTGCAGTAAGAGCGAGATTAGAAGAGATGGGGTATGATTTGAGCCGAGAAGATTTAAATGATGCTT
TTGCTCGTTTTAAGGATTTAGCTGACAGGAAAAGAGAAATTACTGATAGAGATTTAGAGGCAATTGTTAGTGAACAAGTT
CAGCTTCCTGAAGCTAAATTTCAATTAAGTCTTGTCCAAGTAAGTTGCGGTAACGCGTCTAAACCTACTGCCACAATATC
GCTTCTAAACACGGAAGATAATACTGAGGATACCGCCGTATCAATAGGGACTGGCCCCGTTGATGCTGTATGCGAGGCTT
TAAACAAATTAGCTAAAGTTCCTAATGAATTGATTGAATTTTCTGTTAAGTCGGTAACAGAAGGAATTGACGCTTTGGGA
GAAGTAACAATAAGAATAAGAAGGGATAATAAAATATATTCTGGTCATTCTGCTGATACTGATGTTGTAGTTGCCGCAGC
AAATGCTTACGTTAATGCCTTAAATAGGCTTGTATTTTCTGAGAAAAAAAATTCAATCCACCCACAATTTGATAGTTTAG
AAAATTCAACTAATACATTTCTATCCAATAATGCAAATTAA

Upstream 100 bases:

>100_bases
CATTATTGTTTAAGTTACAAACTTGCAGCAAATTTTTATTGAATTAAATCTAGTCAAATATTTTTAATTAGCTTAATATA
AGTTTAGGTTATCCACTTCA

Downstream 100 bases:

>100_bases
ATTAATTTTTTAGGAATATTAAGTAGCATTAAAAAAGCCTCTTTAATATTGTAAATTAATCTAATAGTTAAAGCAGTTAA
TAATGAGAGAAAGATTACTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIANAVGKENGPIICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNINEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDSLENSTNTFLSNNAN

Sequences:

>Translated_546_residues
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIANAVGKENGPIICGLA
RASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEV
IQLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNINEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDSLENSTNTFLSNNAN
>Mature_545_residues
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKAVNKIANAVGKENGPIICGLAR
ASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKSRKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVI
QLAISAGATTINIPDTVGFTTPSEFGNLIADINKNVPNINEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEEMGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQ
LPEAKFQLSLVQVSCGNASKPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALGE
VTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDSLENSTNTFLSNNAN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=46.588693957115, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320019, Length=387, Percent_Identity=28.6821705426357, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=389, Percent_Identity=28.0205655526992, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=556, Percent_Identity=25.5395683453237, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=507, Percent_Identity=24.8520710059172, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59139; Mature: 59008

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKIANAVGKENGPIICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGNLIADINKNVPNINEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLSLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDSLENSTNTF
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCC
LSNNAN
CCCCCC
>Mature Secondary Structure 
SKDPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VNKIANAVGKENGPIICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPSEFGNLIADINKNVPNINEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECHHC
RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLSLVQVSCGNAS
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEEEEEEECCCCC
KPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG
CCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
EVTIRIRRDNKIYSGHSADTDVVVAAANAYVNALNRLVFSEKKNSIHPQFDSLENSTNTF
EEEEEEEECCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCC
LSNNAN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA