Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 123968560

GI number: 123968560

Start: 887131

End: 888483

Strand: Direct

Name: pmbA [H]

Synonym: A9601_10271

Alternate gene names: 123968560

Gene position: 887131-888483 (Clockwise)

Preceding gene: 123968559

Following gene: 123968561

Centisome position: 53.13

GC content: 32.59

Gene sequence:

>1353_bases
ATGAATTCAAAAGAAATAACAACTCAAATCTCTGAAGCTGCAGATTCTCTAAATCTTAGAAAATGGGATTATGGAGCAAG
CTTTTCTAATGATTATTCTGTGCAAGTAGATAATGGTGAGGCTAAACAACTTAAGGCATCACAAAAGCAAATTTTAACTA
TAAGAGTTTGGAACGAATCTAATTTAGTTGGTATTACAACAACTAGTGATATCAGTGAATCTGGTATTAAAAAAGCTCTA
AATCAAGCAAATATTGCATCTGATTTTGGCAACAAGAATGAAAGAACAGAATTTTCACCACTAGCAAAGGATCCAATTGA
AGTTAAGGACTCAAAAAAAAGAAACCCTGTTGGAATAAAAAAATTACTTACTCTTTTAAGAGAAGCGGAAGTAAAACTAT
TAGAAAGCCATGAATCCATAAAATCTGTTCCGTATAATGGTCTATCTGAGAGTTTTTATGAGAGAGTTTATGCAAATAGT
GATGGTGCCTTTCGGAGTTATACTAAAAGTCAAGCTGCGCTTTATTTATACGCAAGAGCAGAAGAGAAAAATAAAAAACC
TCGTAGCTCAGGTTCTGTAAAACTTGGATATGGAGTTGACGATATAGATATAGAATCGTGTATTAAAGACGCTTCTAATA
AAACAATTTCTCATTTAAATTATAAATCTATTAAAACTGATAAATACTTAATATGTTTTTCCCCAGAGTCTTTTTTAACT
ATCATTAATGCCTTTAGTTCAATGTTTAATGCTAGAAGCATTTTAGATGGAGTTAGCTTATCTAATAAAAATTCTATTGG
AGAGAAACTATCTACAGAAGCACTTAACATTTATGATGATGGTCTTCACGAAAAGAATATTTCTTCATCACCATTTGATG
GAGAGGGAACTCCTACCAAAAGACTATGTTTAATTAACAAAGGGCGACTTGAAAATTTTATACATTCTGAATCAACTGCA
AGAATATTCAAAACAATCCCCACAGGCCACGCTGGACTAGGATCAAAAGTCTCAGTATCTCCTGATTGGATAGTAGTTGA
GAAATCAGAGGAAAACTCAGATCTAAAAACATCCTTAGATCACTCTACTTATGAGGGAGAATTTGTTTATATTGAAGAAT
TAAATGCAATCCATGCAGGTGTCAGAGCAAGTCAAGGTTCATTCTCTCTTCCATTTGATGGTTGGCTCTATAAAAACGGT
AAAAAAATCTCAATAGAATCTGCAACTGTAGCTGGGGATATCAAATATCTTTTGAAGAATATATTAAATATTGAATCAAA
CCAGAAAGTAACAACAAGTGGAATTTCTCCACATATATGGGTAGATGAATTATCAATAACTGGTGACGCGTGA

Upstream 100 bases:

>100_bases
AGCTCCCGGATTCTGTGGATCCATTAGTGGAAGTGTCAATGTAACTGTTGGCCAACCTCATATTAAGGTTGATTCAATCA
CTGTTGGCGGAAGATAGGAT

Downstream 100 bases:

>100_bases
GAATTATATTCTGGGGAACACCTGAATATTCAATTGCTAGCCTTGATATTTTTATTAAATCTAAGCACGAAGTAATTGGA
GTAGTTAGCCAACCCGATAA

Product: putative modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNSKEITTQISEAADSLNLRKWDYGASFSNDYSVQVDNGEAKQLKASQKQILTIRVWNESNLVGITTTSDISESGIKKAL
NQANIASDFGNKNERTEFSPLAKDPIEVKDSKKRNPVGIKKLLTLLREAEVKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVDDIDIESCIKDASNKTISHLNYKSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTEALNIYDDGLHEKNISSSPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTIPTGHAGLGSKVSVSPDWIVVEKSEENSDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNILNIESNQKVTTSGISPHIWVDELSITGDA

Sequences:

>Translated_450_residues
MNSKEITTQISEAADSLNLRKWDYGASFSNDYSVQVDNGEAKQLKASQKQILTIRVWNESNLVGITTTSDISESGIKKAL
NQANIASDFGNKNERTEFSPLAKDPIEVKDSKKRNPVGIKKLLTLLREAEVKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVDDIDIESCIKDASNKTISHLNYKSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTEALNIYDDGLHEKNISSSPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTIPTGHAGLGSKVSVSPDWIVVEKSEENSDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNILNIESNQKVTTSGISPHIWVDELSITGDA
>Mature_450_residues
MNSKEITTQISEAADSLNLRKWDYGASFSNDYSVQVDNGEAKQLKASQKQILTIRVWNESNLVGITTTSDISESGIKKAL
NQANIASDFGNKNERTEFSPLAKDPIEVKDSKKRNPVGIKKLLTLLREAEVKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVDDIDIESCIKDASNKTISHLNYKSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTEALNIYDDGLHEKNISSSPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTIPTGHAGLGSKVSVSPDWIVVEKSEENSDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNILNIESNQKVTTSGISPHIWVDELSITGDA

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=425, Percent_Identity=21.1764705882353, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49721; Mature: 49721

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKEITTQISEAADSLNLRKWDYGASFSNDYSVQVDNGEAKQLKASQKQILTIRVWNES
CCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDISESGIKKALNQANIASDFGNKNERTEFSPLAKDPIEVKDSKKRNPVGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KLLTLLREAEVKLLESHESIKSVPYNGLSESFYERVYANSDGAFRSYTKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEC
EEKNKKPRSSGSVKLGYGVDDIDIESCIKDASNKTISHLNYKSIKTDKYLICFSPESFLT
HHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCEEHHCCCCEECCCCEEEEECCHHHHH
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTEALNIYDDGLHEKNISSSPFDGEGTPTK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEHHHCCCCCCCCCCCCCCCCCCCCE
RLCLINKGRLENFIHSESTARIFKTIPTGHAGLGSKVSVSPDWIVVEKSEENSDLKTSLD
EEEEEECCHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHCC
HSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNGKKISIESATVAGDIKYLLKN
CCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHH
ILNIESNQKVTTSGISPHIWVDELSITGDA
HHCCCCCCEEEECCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MNSKEITTQISEAADSLNLRKWDYGASFSNDYSVQVDNGEAKQLKASQKQILTIRVWNES
CCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDISESGIKKALNQANIASDFGNKNERTEFSPLAKDPIEVKDSKKRNPVGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KLLTLLREAEVKLLESHESIKSVPYNGLSESFYERVYANSDGAFRSYTKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEC
EEKNKKPRSSGSVKLGYGVDDIDIESCIKDASNKTISHLNYKSIKTDKYLICFSPESFLT
HHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCEEHHCCCCEECCCCEEEEECCHHHHH
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTEALNIYDDGLHEKNISSSPFDGEGTPTK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEHHHCCCCCCCCCCCCCCCCCCCCE
RLCLINKGRLENFIHSESTARIFKTIPTGHAGLGSKVSVSPDWIVVEKSEENSDLKTSLD
EEEEEECCHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHCC
HSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNGKKISIESATVAGDIKYLLKN
CCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHH
ILNIESNQKVTTSGISPHIWVDELSITGDA
HHCCCCCCEEEECCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]