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The map label for this gene is metG [H]

Identifier: 123968528

GI number: 123968528

Start: 866496

End: 868040

Strand: Direct

Name: metG [H]

Synonym: A9601_09951

Alternate gene names: 123968528

Gene position: 866496-868040 (Clockwise)

Preceding gene: 123968527

Following gene: 123968529

Centisome position: 51.89

GC content: 30.23

Gene sequence:

>1545_bases
ATGACTTTTGTCATTACTACACCTTTATACTATGTTAATGATAAACCTCATTTAGGAAGTGTATATACAACAATAATTTG
TGACTCAATAGCTAGGTATAAAAGGCTTGCAGGTGAAGAAGTTATTTTCATCACTGGTGTCGATGAACATGGTTTAAAAA
TACAAAGAACAGCTAATGAAAAGGGTATTCCTCCAAAATCACATTGTGATGAAATCTCAGAAATCTTTAATAATAATTGG
AAAGATTGGAATATATCCTTTGACAAATTTATAAGAACAAGCTCAAAAAATCATGAATTTGTTGTTAATGAATTTTATGA
AAGAGTAAAAGCATCAGATGATATCTATATGGGAGTTCAAAAAGGTTGGTATTGTGTCGGTTGCGAAGAATTTAAAGATA
ATCCAGAAAATTCATCAACATACAAATGTCCCATACATCAAAAAAATCTTGAATGGAAAAATGAAGAGAATCTCTTTTTT
AGGCTTTCAAAATATCAAAAAGAAATCGAGAAAATAATCAACGAACCTTCTTTTATAGAGCCAATAGAAAGAAAGAATGA
AATTATAAATTTTGTTTCTAGAGGTTTAAAGGATTTTTCAATTTCAAGAACAAATGTTACATGGGGAATTCCAGTCCCTG
GTTACGATAACCATACCTTTTATGTGTGGTTTGATGCTTTACTTGGATATGTAAGTGCCATTAGTTCTGATGCGACAGAA
CATTCATTGGAAAAATCAATTAATGGAGGATGGCCAGCTGATGTTCATTTAATTGGTAAGGATATTCTGAGATTCCATGC
TGTATATTGGCCTGCAATGCTCATTTCTGCCAAAATGAAAGTTCCTAAGAAGGTTTTTGGGCATGGGTTTCTTACAAGAG
AGGGGCAAAAAATGGGTAAAAGCTTAGGAAATGTACTCGACCCTGATTTATTGCTTAAAAAATATGGAAATGATCCTGTA
AGGTGGTACCTCATTAAAGACATATCATTAGGAAATGATGGGGATTTTCAAGATAAAAGATTTGTTGACATCATCAATAA
TGACTTAGCTAATACAATAGGTAATTTATTAAATAGAACATCATCTATGTCTAGAAAATGGTTTGATAATAAAGTGCCAA
ATAATGAAAAAATTTCAAGTGAAAATAAATTAGAGAATTATGCCAAAATTGCAGTTGAAAACTATATTTATAACTTTGAT
AACTATAAATTAGATTTAGCAGCTAATGAAGTACTTAGCCTAGCAATTAATACAAATTTGTATTTGAATGATAATCAGCC
ATGGGTGCTAATAAAAGAGAAAGATAATCTACCTCTAGTTAAAGAAATTATTTATAACGTTTTAGAAAGTACCAGAATAA
TAGGATTATTATTACTACCTTTATTGCCCGAATTATCTATAAAAATTAATGAACAACTTGGCTCTATATACAGAGAGGAG
ATTTCTTGGAAAAAACAATTAATTTGGGGATTATTAGTTAGCAACTCAAGTCTTCCTAAACCCACTCCAATCATAAATAA
ACTTGAGTATGAGCAAAAATTATAG

Upstream 100 bases:

>100_bases
ATGATAGTAATCTTTTAGAATTTATTTATTAATAAGATAATCAATATAATGGGTTATTATTAGTTTAAAAAATATCTGTT
AGTATAAATATAAAAGCTTT

Downstream 100 bases:

>100_bases
ATTAATAATTTTCCTTACATTATTTATGCTTGGTTGCTCTCCAAGTGTAATTGATGAAAAAAAAGTTATCCAAAAAATAG
ACAGTTTAGATATGACTATA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEEVIFITGVDEHGLKIQRTANEKGIPPKSHCDEISEIFNNNW
KDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVTWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLKKYGNDPV
RWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPNNEKISSENKLENYAKIAVENYIYNFD
NYKLDLAANEVLSLAINTNLYLNDNQPWVLIKEKDNLPLVKEIIYNVLESTRIIGLLLLPLLPELSIKINEQLGSIYREE
ISWKKQLIWGLLVSNSSLPKPTPIINKLEYEQKL

Sequences:

>Translated_514_residues
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEEVIFITGVDEHGLKIQRTANEKGIPPKSHCDEISEIFNNNW
KDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFF
RLSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVTWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLKKYGNDPV
RWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPNNEKISSENKLENYAKIAVENYIYNFD
NYKLDLAANEVLSLAINTNLYLNDNQPWVLIKEKDNLPLVKEIIYNVLESTRIIGLLLLPLLPELSIKINEQLGSIYREE
ISWKKQLIWGLLVSNSSLPKPTPIINKLEYEQKL
>Mature_513_residues
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEEVIFITGVDEHGLKIQRTANEKGIPPKSHCDEISEIFNNNWK
DWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQKGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFR
LSKYQKEIEKIINEPSFIEPIERKNEIINFVSRGLKDFSISRTNVTWGIPVPGYDNHTFYVWFDALLGYVSAISSDATEH
SLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGKSLGNVLDPDLLLKKYGNDPVR
WYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPNNEKISSENKLENYAKIAVENYIYNFDN
YKLDLAANEVLSLAINTNLYLNDNQPWVLIKEKDNLPLVKEIIYNVLESTRIIGLLLLPLLPELSIKINEQLGSIYREEI
SWKKQLIWGLLVSNSSLPKPTPIINKLEYEQKL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=501, Percent_Identity=34.3313373253493, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=25.5769230769231, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1788432, Length=514, Percent_Identity=26.4591439688716, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI212645096, Length=504, Percent_Identity=30.952380952381, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71992781, Length=344, Percent_Identity=30.5232558139535, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=24.8648648648649, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321610, Length=511, Percent_Identity=34.0508806262231, Blast_Score=256, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=521, Percent_Identity=23.8003838771593, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21358483, Length=489, Percent_Identity=30.6748466257669, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24655311, Length=548, Percent_Identity=27.1897810218978, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59432; Mature: 59300

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEEVIFITGVDEHGLKIQRTANE
CEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCC
KGIPPKSHCDEISEIFNNNWKDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIEKIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVTWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HHHHHHHHHHHHHCCCHHCEEEECEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHCCHHH
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLKKYGNDPVRWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRT
HHHCCCCHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFDNKVPNNEKISSENKLENYAKIAVENYIYNFDNYKLDLAANEVLSLAINTNL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCEEEEEEECEE
YLNDNQPWVLIKEKDNLPLVKEIIYNVLESTRIIGLLLLPLLPELSIKINEQLGSIYREE
EECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
ISWKKQLIWGLLVSNSSLPKPTPIINKLEYEQKL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TFVITTPLYYVNDKPHLGSVYTTIICDSIARYKRLAGEEVIFITGVDEHGLKIQRTANE
EEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCC
KGIPPKSHCDEISEIFNNNWKDWNISFDKFIRTSSKNHEFVVNEFYERVKASDDIYMGVQ
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKDNPENSSTYKCPIHQKNLEWKNEENLFFRLSKYQKEIEKIINEPSFIE
CCEEEEECHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCC
PIERKNEIINFVSRGLKDFSISRTNVTWGIPVPGYDNHTFYVWFDALLGYVSAISSDATE
HHHHHHHHHHHHHCCCHHCEEEECEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHCCHHH
HSLEKSINGGWPADVHLIGKDILRFHAVYWPAMLISAKMKVPKKVFGHGFLTREGQKMGK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHH
SLGNVLDPDLLLKKYGNDPVRWYLIKDISLGNDGDFQDKRFVDIINNDLANTIGNLLNRT
HHHCCCCHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SSMSRKWFDNKVPNNEKISSENKLENYAKIAVENYIYNFDNYKLDLAANEVLSLAINTNL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCEEEEEEECEE
YLNDNQPWVLIKEKDNLPLVKEIIYNVLESTRIIGLLLLPLLPELSIKINEQLGSIYREE
EECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
ISWKKQLIWGLLVSNSSLPKPTPIINKLEYEQKL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]