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The map label for this gene is cca [H]

Identifier: 123967863

GI number: 123967863

Start: 299676

End: 300923

Strand: Reverse

Name: cca [H]

Synonym: A9601_03261

Alternate gene names: 123967863

Gene position: 300923-299676 (Counterclockwise)

Preceding gene: 123967864

Following gene: 123967861

Centisome position: 18.02

GC content: 28.29

Gene sequence:

>1248_bases
ATGAACGATATCTCTGATTACATCCAAGGAGAATTAATCAAAACTCCATTTAATCTATATAACCTTATTACTAAATACAT
AGAATCTAATAACAATACTAAAGTGGCTTTTGTTGGCGGTTATTTAAGAGATTTGTTAATTAGTAAATTCCACAAAAAAT
CGTTTTCTGAACCTGTAGATATTGATCTTGTTATTGAAGGATCCTCTATTGCTCTTGCAAAATTTATAAAAAAAAACATT
GTAAATGTAGATTTATGTTTGATCAAAGAATTTAATTTATACAACACTGTAGAAATAAATATTAATGACTATAAAATTGA
TATTGCTTCTGCAAGAAAAGAAATTTATTCTGCTCCAGGCTTAAATCCCACAGTAAATAAAAGTACTATCGAGGAGGATC
TTAAGAGGAGAGATTTCTCTATAAATTCAATAGCCTTCGAGGTCTCGAAAAGGGAAATCTATGATCTTTATGGAGGAATT
TCTGATATAAAAAGTAAAACATTGAACTTACTTCACAGTAATAGTATTTCAGATGATCCAAGTAGATTAATTAGATGTGC
AAAATATGCCTCAAGGTTAGATTTCAATATTTCAAATGATACCCTGAAACAATCTCAAGAAACAGTTAGACAATGGCCAT
GGAAAAGTTCAGAAACTCATCAGAAAATGATTTACCCTCCTGCAATAGGCATGCGAATAAGGATGGAACTAGCTGAAATA
TGCAAACACGATAATTTGACTAATGTAATTTCGATAATTAATAAATGGGAAGTTATCTCAATCTTAAATGAAAATATTAA
AGTCGATAAACGGTTTTTAAGAGGACTAAATTGGATTAAGAAGTTAAAGGGAAATCATATGCTTTACTTATTAAAAGATT
CAGAAGATTTAGAAAAAGGATGTCAAAGATTTTTAGTGAATAATAGTGAGATAAAAATATTAGAAGATTATTTAAATATC
AAAAAGATATTAAATAAAAACCAAAAAGATTTCAATCATTTTTCTCCATCAAGTTGGACAGAATTTATTGAGGACAGAAA
CCTTAATGATGAGACAGTCAAATTATTAATTTGTGATGGAGGACCATACTGGCGTAAATTGTTTAAGTGGTTATTTATTT
ACAAATTCATAAAATCAAAAAAAGATGGAGAAACATTAAAAAAAGAGGGATGGGAACCAGGGAAGGAGATGGGAAAGGAA
ATCAAAAGATTAAGATATTTAGAAATTGACAAATTAAATAGAAATTAA

Upstream 100 bases:

>100_bases
GGAGTGGGGAAAAAATATCTTTAGCAGTAAAATTTGGTGATAAAGGAAGTAAAATTCTAGATCCCAGATTAGCTCCAATT
CGTTATGTAAGTTAAAAATC

Downstream 100 bases:

>100_bases
TTACATTTTTACAACCTCTCCAACCTTGTACCATTTATCCTTTAGAACATTTTCATATTTACGGTTGCAACTAATCAACA
AGGGGCCACATGTTTGAGGA

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNDISDYIQGELIKTPFNLYNLITKYIESNNNTKVAFVGGYLRDLLISKFHKKSFSEPVDIDLVIEGSSIALAKFIKKNI
VNVDLCLIKEFNLYNTVEININDYKIDIASARKEIYSAPGLNPTVNKSTIEEDLKRRDFSINSIAFEVSKREIYDLYGGI
SDIKSKTLNLLHSNSISDDPSRLIRCAKYASRLDFNISNDTLKQSQETVRQWPWKSSETHQKMIYPPAIGMRIRMELAEI
CKHDNLTNVISIINKWEVISILNENIKVDKRFLRGLNWIKKLKGNHMLYLLKDSEDLEKGCQRFLVNNSEIKILEDYLNI
KKILNKNQKDFNHFSPSSWTEFIEDRNLNDETVKLLICDGGPYWRKLFKWLFIYKFIKSKKDGETLKKEGWEPGKEMGKE
IKRLRYLEIDKLNRN

Sequences:

>Translated_415_residues
MNDISDYIQGELIKTPFNLYNLITKYIESNNNTKVAFVGGYLRDLLISKFHKKSFSEPVDIDLVIEGSSIALAKFIKKNI
VNVDLCLIKEFNLYNTVEININDYKIDIASARKEIYSAPGLNPTVNKSTIEEDLKRRDFSINSIAFEVSKREIYDLYGGI
SDIKSKTLNLLHSNSISDDPSRLIRCAKYASRLDFNISNDTLKQSQETVRQWPWKSSETHQKMIYPPAIGMRIRMELAEI
CKHDNLTNVISIINKWEVISILNENIKVDKRFLRGLNWIKKLKGNHMLYLLKDSEDLEKGCQRFLVNNSEIKILEDYLNI
KKILNKNQKDFNHFSPSSWTEFIEDRNLNDETVKLLICDGGPYWRKLFKWLFIYKFIKSKKDGETLKKEGWEPGKEMGKE
IKRLRYLEIDKLNRN
>Mature_415_residues
MNDISDYIQGELIKTPFNLYNLITKYIESNNNTKVAFVGGYLRDLLISKFHKKSFSEPVDIDLVIEGSSIALAKFIKKNI
VNVDLCLIKEFNLYNTVEININDYKIDIASARKEIYSAPGLNPTVNKSTIEEDLKRRDFSINSIAFEVSKREIYDLYGGI
SDIKSKTLNLLHSNSISDDPSRLIRCAKYASRLDFNISNDTLKQSQETVRQWPWKSSETHQKMIYPPAIGMRIRMELAEI
CKHDNLTNVISIINKWEVISILNENIKVDKRFLRGLNWIKKLKGNHMLYLLKDSEDLEKGCQRFLVNNSEIKILEDYLNI
KKILNKNQKDFNHFSPSSWTEFIEDRNLNDETVKLLICDGGPYWRKLFKWLFIYKFIKSKKDGETLKKEGWEPGKEMGKE
IKRLRYLEIDKLNRN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48546; Mature: 48546

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDISDYIQGELIKTPFNLYNLITKYIESNNNTKVAFVGGYLRDLLISKFHKKSFSEPVD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCC
IDLVIEGSSIALAKFIKKNIVNVDLCLIKEFNLYNTVEININDYKIDIASARKEIYSAPG
EEEEEECCCHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECEEEEEHHHHHHHHHCCCC
LNPTVNKSTIEEDLKRRDFSINSIAFEVSKREIYDLYGGISDIKSKTLNLLHSNSISDDP
CCCCCCHHHHHHHHHHCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCH
SRLIRCAKYASRLDFNISNDTLKQSQETVRQWPWKSSETHQKMIYPPAIGMRIRMELAEI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHH
CKHDNLTNVISIINKWEVISILNENIKVDKRFLRGLNWIKKLKGNHMLYLLKDSEDLEKG
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHH
CQRFLVNNSEIKILEDYLNIKKILNKNQKDFNHFSPSSWTEFIEDRNLNDETVKLLICDG
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECC
GPYWRKLFKWLFIYKFIKSKKDGETLKKEGWEPGKEMGKEIKRLRYLEIDKLNRN
CHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDISDYIQGELIKTPFNLYNLITKYIESNNNTKVAFVGGYLRDLLISKFHKKSFSEPVD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCC
IDLVIEGSSIALAKFIKKNIVNVDLCLIKEFNLYNTVEININDYKIDIASARKEIYSAPG
EEEEEECCCHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECEEEEEHHHHHHHHHCCCC
LNPTVNKSTIEEDLKRRDFSINSIAFEVSKREIYDLYGGISDIKSKTLNLLHSNSISDDP
CCCCCCHHHHHHHHHHCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCH
SRLIRCAKYASRLDFNISNDTLKQSQETVRQWPWKSSETHQKMIYPPAIGMRIRMELAEI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHH
CKHDNLTNVISIINKWEVISILNENIKVDKRFLRGLNWIKKLKGNHMLYLLKDSEDLEKG
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHH
CQRFLVNNSEIKILEDYLNIKKILNKNQKDFNHFSPSSWTEFIEDRNLNDETVKLLICDG
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECC
GPYWRKLFKWLFIYKFIKSKKDGETLKKEGWEPGKEMGKEIKRLRYLEIDKLNRN
CHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA