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The map label for this gene is phrB [H]

Identifier: 123967845

GI number: 123967845

Start: 284525

End: 285958

Strand: Reverse

Name: phrB [H]

Synonym: A9601_03081

Alternate gene names: 123967845

Gene position: 285958-284525 (Counterclockwise)

Preceding gene: 123967846

Following gene: 123967842

Centisome position: 17.12

GC content: 30.54

Gene sequence:

>1434_bases
ATGAATAAACCTAGAATACTTTTCTGGCATAGAAAGGATTTAAGAATATTTGATAATCAAGCTTTAATCAAAGCATTTTC
ATTATCAAATGCTATTACTTCAACCTATATATTTGATAAAAATTACTCACACGATTTCAATGCAAGTTCAAGAGCTTGGT
TTCTAGGAAATTCACTTCAAGAATTAGGAAATAATTGGAAAAAAATGGGTAGTAGATTAGTTATGGAAGAAGGAGATCCG
GTATTAATAATTCCCAAATTAGCAAAGAAAATAAATGCTAAATTTGTTTTTTGGAATAGATCAATTGAACCTTATGAGAT
TAATCGCGATTTACAAATAAAAAAAAATTTAAAAGAACAAAACATTCAAGTTATTGAAACTTGGGATCACTTATTAGTAG
AACCTTTAAAAATATTTTCAGGGAATAATAATCCTTATTCAGTTTATGGACCTTTTTATAAAAACCTTAAATCAAAAATG
AATTTATTAGGTTTATATGAACAAGATAAAGTTGGTTTCCAGTTTAAAGATATTGATAATAAACTCAAAGATAAGACAAT
AAATTCATCTGATTCGGTTTTAGAGAAATTTATCAAAAATATCAAATTTCCTGGTTCGAATATTTGTCCATGTAAACCTG
GAGAGAATGCTGCAGAAACATTATTAGAAAACTTCATTAACGAAAAAAAAATATATTCTTATAATTCTGCACGAGATTTT
CCTTCCCATAATGGGACATCTTTTCTAAGTGCATCTCTCAGATTCGGTACCATCAGCATTAGAAAAGTTTGGAACGCCAC
TTTAAATTTAAATTCAGATTTGGAAAATCAAGGAAATTATCTATCAATTGAAACTTGGCAAAAAGAACTTGTTTGGCGTG
AATTTTATCAACATTGCTTATTCCATTTCCCAGAGCTAGAGAAAGGTCCCTATAGAAAAAAATGGGATCACTTTCGATGG
CAAAACAATAATGAATGGTTTCAGCATTGGAGCAACGGAGAGACCGGAGTACCTATAGTTGATGCTGCAATGCGTCAACT
AAATAGTACTGGCTGGATGCATAACAGATGTAGGATGATAGTCGCTTCATTTCTGGTAAAAGATCTTATATGCAATTGGC
AAATGGGCGAGAAAAAATTTATGGAGACTTTGGTTGATGGAGACTTAGCTGCAAATAATGGGGGATGGCAGTGGAGCGCA
AGTAGCGGTATGGATCCAAAACCTCTTAGAATTTTTAATCCATATACCCAAGCAAAAAAATTTGATCCTATTTGCGAATA
TATAAAATATTGGATTCCTGAATTATCTAAAGTGTCAAATTCAGAATTATTAAATGGGGATATATCTAATTTAGAAAAAA
ATGATTATTCAAGCCCTATTGTCAATCACAAGATACAACAAAGATTATTTAAATCACTTTATGCTGAAATTTGA

Upstream 100 bases:

>100_bases
ATCCCGACGAACTAGATAATCTTATTTCTAGTGGGGATGAAATTCTTGACGCAAAAACCGTGACGGCTTGGTTTAGAGCT
AAACAATTTTTAGATAAATT

Downstream 100 bases:

>100_bases
ATTTCCTCTATACAATTCTTTAAAACTTTATTTAGATTTTCTGCTACATAAACTTGCTCTTCATAAGAAATTTCAGGAAA
CATTGGCAGACTAAGAACTT

Product: putative DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNKPRILFWHRKDLRIFDNQALIKAFSLSNAITSTYIFDKNYSHDFNASSRAWFLGNSLQELGNNWKKMGSRLVMEEGDP
VLIIPKLAKKINAKFVFWNRSIEPYEINRDLQIKKNLKEQNIQVIETWDHLLVEPLKIFSGNNNPYSVYGPFYKNLKSKM
NLLGLYEQDKVGFQFKDIDNKLKDKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETLLENFINEKKIYSYNSARDF
PSHNGTSFLSASLRFGTISIRKVWNATLNLNSDLENQGNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFRW
QNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSA
SSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGDISNLEKNDYSSPIVNHKIQQRLFKSLYAEI

Sequences:

>Translated_477_residues
MNKPRILFWHRKDLRIFDNQALIKAFSLSNAITSTYIFDKNYSHDFNASSRAWFLGNSLQELGNNWKKMGSRLVMEEGDP
VLIIPKLAKKINAKFVFWNRSIEPYEINRDLQIKKNLKEQNIQVIETWDHLLVEPLKIFSGNNNPYSVYGPFYKNLKSKM
NLLGLYEQDKVGFQFKDIDNKLKDKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETLLENFINEKKIYSYNSARDF
PSHNGTSFLSASLRFGTISIRKVWNATLNLNSDLENQGNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFRW
QNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSA
SSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGDISNLEKNDYSSPIVNHKIQQRLFKSLYAEI
>Mature_477_residues
MNKPRILFWHRKDLRIFDNQALIKAFSLSNAITSTYIFDKNYSHDFNASSRAWFLGNSLQELGNNWKKMGSRLVMEEGDP
VLIIPKLAKKINAKFVFWNRSIEPYEINRDLQIKKNLKEQNIQVIETWDHLLVEPLKIFSGNNNPYSVYGPFYKNLKSKM
NLLGLYEQDKVGFQFKDIDNKLKDKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETLLENFINEKKIYSYNSARDF
PSHNGTSFLSASLRFGTISIRKVWNATLNLNSDLENQGNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFRW
QNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSA
SSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGDISNLEKNDYSSPIVNHKIQQRLFKSLYAEI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=496, Percent_Identity=26.6129032258064, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4758072, Length=500, Percent_Identity=28.6, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI188536103, Length=452, Percent_Identity=26.3274336283186, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1786926, Length=474, Percent_Identity=37.3417721518987, Blast_Score=256, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324962, Length=484, Percent_Identity=31.198347107438, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17137248, Length=454, Percent_Identity=27.7533039647577, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24585455, Length=454, Percent_Identity=27.7533039647577, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24648152, Length=467, Percent_Identity=26.7665952890792, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55817; Mature: 55817

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPRILFWHRKDLRIFDNQALIKAFSLSNAITSTYIFDKNYSHDFNASSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCHHHH
ELGNNWKKMGSRLVMEEGDPVLIIPKLAKKINAKFVFWNRSIEPYEINRDLQIKKNLKEQ
HHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCCEEEEECCCCCCEECCCCCHHHHCHHHC
NIQVIETWDHLLVEPLKIFSGNNNPYSVYGPFYKNLKSKMNLLGLYEQDKVGFQFKDIDN
CCEEEEHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEHHHHH
KLKDKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETLLENFINEKKIYSYNSARDF
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
PSHNGTSFLSASLRFGTISIRKVWNATLNLNSDLENQGNYLSIETWQKELVWREFYQHCL
CCCCCCHHEEEEEEECEEEEEEHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
FHFPELEKGPYRKKWDHFRWQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMI
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
VASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQAKK
HHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEEECCHHHHHC
FDPICEYIKYWIPELSKVSNSELLNGDISNLEKNDYSSPIVNHKIQQRLFKSLYAEI
CCHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKPRILFWHRKDLRIFDNQALIKAFSLSNAITSTYIFDKNYSHDFNASSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCHHHH
ELGNNWKKMGSRLVMEEGDPVLIIPKLAKKINAKFVFWNRSIEPYEINRDLQIKKNLKEQ
HHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCCEEEEECCCCCCEECCCCCHHHHCHHHC
NIQVIETWDHLLVEPLKIFSGNNNPYSVYGPFYKNLKSKMNLLGLYEQDKVGFQFKDIDN
CCEEEEHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEHHHHH
KLKDKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETLLENFINEKKIYSYNSARDF
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
PSHNGTSFLSASLRFGTISIRKVWNATLNLNSDLENQGNYLSIETWQKELVWREFYQHCL
CCCCCCHHEEEEEEECEEEEEEHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
FHFPELEKGPYRKKWDHFRWQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMI
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
VASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQAKK
HHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEEECCHHHHHC
FDPICEYIKYWIPELSKVSNSELLNGDISNLEKNDYSSPIVNHKIQQRLFKSLYAEI
CCHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]