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The map label for this gene is cobS [H]

Identifier: 123967829

GI number: 123967829

Start: 269976

End: 270716

Strand: Reverse

Name: cobS [H]

Synonym: A9601_02921

Alternate gene names: 123967829

Gene position: 270716-269976 (Counterclockwise)

Preceding gene: 123967832

Following gene: 123967827

Centisome position: 16.21

GC content: 29.15

Gene sequence:

>741_bases
TTGGCAGGATCTTGGATTTTCTATACAACATTTCCAAAGATACCTTTAATTAATCCCGAATTTAAAAATATTGCACAATT
TGCCCCGCCTTTAGGATTTTTGATTGGAACAATACAGAGTTATATTTTTCTTTTTTTTGCAACAAACTCTTGGTCAATTT
ATGCATCTGCATTAATTTGTTTAGCTTCAGGATATTTAATTACTGGTGGTCTACACATTGATGGTTTGATGGATACTTTC
GATGGTATTTTTGCGGGTAAAAAGAAACGCTTAAAAGCCATGAAAGACAGTAAAGTTGGTTCCTTTGGCGTACAAGCTTT
AATTTTTATAACTCTAATTCAAATTGCTTGCATACTGAAAATTCAAAGCCTAATAATTTTTGTTTTACCTATATGCTTAT
TTTGGGGAAGATTTTCAAATTTATTTTTTATAGAAAAGTTTAAATATATGAGTTATAAGAAAAAATCTATTAGTCACAAA
AAGTTTTGGAATGGATTTAAAAAAGAATCTTTGATCTCAATTATTTTTCTTTTAATTTTCATTTCATACCAATTAGTTTC
AATTACATCCCAAACAATATTAATTAAATTTTTAATTCTTATTTTGATTGGTATCTTTCTAAGCTATTCTATCCCAAACA
TACTGGGTAATAAAATTGGAGGCTTCAACGGAGATGCCTGCGGCGCAAGTGTTGTACTAGTTGAAACTGCAATGTTGTTT
ATGCATGCAATTCTTTTATAG

Upstream 100 bases:

>100_bases
ACGATAATTTATAAAATTTTTTGATCCTTTTTCATTAATCTACCCTATTATTTAATATTGAATCTATTTTTATATCATCA
AAAAATATTTAATAAAAAAT

Downstream 100 bases:

>100_bases
GTAGTTCTAAATAGAAAATATTTTTCTTCTTAAGATTTTTTGATTTCGCAGTATTATCAACCGAGTTATTTTCCAGCAAT
CTCAAATCTCCTCCAATTTG

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAGSWIFYTTFPKIPLINPEFKNIAQFAPPLGFLIGTIQSYIFLFFATNSWSIYASALICLASGYLITGGLHIDGLMDTF
DGIFAGKKKRLKAMKDSKVGSFGVQALIFITLIQIACILKIQSLIIFVLPICLFWGRFSNLFFIEKFKYMSYKKKSISHK
KFWNGFKKESLISIIFLLIFISYQLVSITSQTILIKFLILILIGIFLSYSIPNILGNKIGGFNGDACGASVVLVETAMLF
MHAILL

Sequences:

>Translated_246_residues
MAGSWIFYTTFPKIPLINPEFKNIAQFAPPLGFLIGTIQSYIFLFFATNSWSIYASALICLASGYLITGGLHIDGLMDTF
DGIFAGKKKRLKAMKDSKVGSFGVQALIFITLIQIACILKIQSLIIFVLPICLFWGRFSNLFFIEKFKYMSYKKKSISHK
KFWNGFKKESLISIIFLLIFISYQLVSITSQTILIKFLILILIGIFLSYSIPNILGNKIGGFNGDACGASVVLVETAMLF
MHAILL
>Mature_245_residues
AGSWIFYTTFPKIPLINPEFKNIAQFAPPLGFLIGTIQSYIFLFFATNSWSIYASALICLASGYLITGGLHIDGLMDTFD
GIFAGKKKRLKAMKDSKVGSFGVQALIFITLIQIACILKIQSLIIFVLPICLFWGRFSNLFFIEKFKYMSYKKKSISHKK
FWNGFKKESLISIIFLLIFISYQLVSITSQTILIKFLILILIGIFLSYSIPNILGNKIGGFNGDACGASVVLVETAMLFM
HAILL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27569; Mature: 27437

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSWIFYTTFPKIPLINPEFKNIAQFAPPLGFLIGTIQSYIFLFFATNSWSIYASALIC
CCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LASGYLITGGLHIDGLMDTFDGIFAGKKKRLKAMKDSKVGSFGVQALIFITLIQIACILK
HHCCCEEECCEEEHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IQSLIIFVLPICLFWGRFSNLFFIEKFKYMSYKKKSISHKKFWNGFKKESLISIIFLLIF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHH
ISYQLVSITSQTILIKFLILILIGIFLSYSIPNILGNKIGGFNGDACGASVVLVETAMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
MHAILL
HHHHHC
>Mature Secondary Structure 
AGSWIFYTTFPKIPLINPEFKNIAQFAPPLGFLIGTIQSYIFLFFATNSWSIYASALIC
CCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LASGYLITGGLHIDGLMDTFDGIFAGKKKRLKAMKDSKVGSFGVQALIFITLIQIACILK
HHCCCEEECCEEEHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IQSLIIFVLPICLFWGRFSNLFFIEKFKYMSYKKKSISHKKFWNGFKKESLISIIFLLIF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHH
ISYQLVSITSQTILIKFLILILIGIFLSYSIPNILGNKIGGFNGDACGASVVLVETAMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
MHAILL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]