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The map label for this gene is leuC [H]

Identifier: 123967815

GI number: 123967815

Start: 255305

End: 256711

Strand: Reverse

Name: leuC [H]

Synonym: A9601_02781

Alternate gene names: 123967815

Gene position: 256711-255305 (Counterclockwise)

Preceding gene: 123967816

Following gene: 123967814

Centisome position: 15.37

GC content: 36.6

Gene sequence:

>1407_bases
TTGAGTCAAGATACCTTATTTGATAAAGTTTGGGATTTACACAAAGTTTCAAGACTTCCAGGTGGATCCGATCAAATATT
AATTGGTCTTCACCTTATCCATGAAGTTACAAGTCCTCAAGCATTTGGCGCTTTAAAAGACAAAAACTTAAAAGTTAAAT
TCCCTGATAGAACTGTTGCTACCGTTGATCATATTGTGCCCACAGACAATCAGAGCAGGCCTTTCAAAGATAATCTCGCT
GAACAGATGATTGAAACACTTGAGAAGAACTGCTTAGAGCATAAAATAAGATTTTTTAATATTGGTAGTGGTAATCAAGG
AATAGTTCATGTAGTAGCACCAGAATTAGGGCTTACTCAGCCAGGGATGACAATCGCTTGCGGAGATTCTCATACTTCAA
CTCATGGAGCTTTTGGGTCAATTGCTTTTGGGATAGGTACAAGTCAAGTAAGAGACGTTCTTGCTACCCAAACCATAGCC
ATGAACAAATTGAAAGTAAGGCAGATTTGGTGTGATAATAAATTATCAAAGGGTGTTTTTGCTAAGGATTTAGTACTTCA
TATCATTAATGAACTTGGTGTAAAAGCAGGAGTAGGTTTCGCATATGAATTCGCAGGACCTGCTATAGATGAATTATCAA
TGGAAGAAAGGATGACGATATGCAATATGTCTATTGAAGGAGGAGCAAGATGCGGTTACATCAATCCTGATGAAAAAACA
TTTAGTTATATTAAAGATAAATTATGTGCTCCTAAAAATGAGTATTGGGATAAAGCACTAACATGGTGGAAATCATTAAA
AAGCGATGAAAATTCAATTTATGATGATGTAATTAAATTAGATGCTTCTAAAGTGGAGCCAACCGTAACCTGGGGGATTA
CTCCTGGCCAAAGTATAAGCATTAATCAACAAATCCCTCTTTTAGATGACTTGTCCCCAAATGATAAATTAGTTGCAAAA
GAGGCTTATGAATATATGAGTTTCAAGCCAGGGCAATATATCAAAGATACTCCTGTTGATGTTTGTTTCATAGGTAGCTG
CACAAATGGAAGAATCAGTGACTTAAGAGTTGCAGCTAGAGTATTAAAAAACAAAAAAGTATCTAAGAATGTTAAAGCAT
TTGTAGTACCAGGTTCAGAAAAAGTGGCAACTGAAGCAAAACAAGAAGGTCTTGATGAGATATTTAAGGATTCTGGATTT
CAATGGAGAGAGCCTGGCTGCTCAATGTGTTTAGCAATGAATTCAGATAAGCTAATAGGTAATCAAGTTAGTGCTAGTTC
TAGTAATAGAAATTTCAAGGGTAGGCAAGGATCTCCCAGTGGGAGAACACTGCTCATGAGTCCAGCAATGGTTGCTGCTG
CTGCAATTAATGGCAAAGTTAGTGACGTTAGAGAATTTATCAACTGA

Upstream 100 bases:

>100_bases
ACCCGAAATAGAGTAGAAATTCAAACACTAAGTGTAAATGTGTGTTTGAACAGCCTCAGATTAATCCTATTATCTAATAG
TAAGTAACTATTTTTACAAA

Downstream 100 bases:

>100_bases
TGAAATCAGAGTTTACCCCTCCAATTGGAAAAATTTCAAATATTAACGGGCAATGTATCCCATTGATTGGTAATGATATT
GATACAGACCGAATAATTCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSQDTLFDKVWDLHKVSRLPGGSDQILIGLHLIHEVTSPQAFGALKDKNLKVKFPDRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLEKNCLEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCDNKLSKGVFAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKDKLCAPKNEYWDKALTWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSISINQQIPLLDDLSPNDKLVAK
EAYEYMSFKPGQYIKDTPVDVCFIGSCTNGRISDLRVAARVLKNKKVSKNVKAFVVPGSEKVATEAKQEGLDEIFKDSGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFIN

Sequences:

>Translated_468_residues
MSQDTLFDKVWDLHKVSRLPGGSDQILIGLHLIHEVTSPQAFGALKDKNLKVKFPDRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLEKNCLEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCDNKLSKGVFAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKDKLCAPKNEYWDKALTWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSISINQQIPLLDDLSPNDKLVAK
EAYEYMSFKPGQYIKDTPVDVCFIGSCTNGRISDLRVAARVLKNKKVSKNVKAFVVPGSEKVATEAKQEGLDEIFKDSGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFIN
>Mature_467_residues
SQDTLFDKVWDLHKVSRLPGGSDQILIGLHLIHEVTSPQAFGALKDKNLKVKFPDRTVATVDHIVPTDNQSRPFKDNLAE
QMIETLEKNCLEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAM
NKLKVRQIWCDNKLSKGVFAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKTF
SYIKDKLCAPKNEYWDKALTWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSISINQQIPLLDDLSPNDKLVAKE
AYEYMSFKPGQYIKDTPVDVCFIGSCTNGRISDLRVAARVLKNKKVSKNVKAFVVPGSEKVATEAKQEGLDEIFKDSGFQ
WREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFIN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=397, Percent_Identity=26.1964735516373, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI8659555, Length=413, Percent_Identity=24.6973365617433, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI41352693, Length=369, Percent_Identity=24.6612466124661, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786259, Length=461, Percent_Identity=49.2407809110629, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1787531, Length=445, Percent_Identity=25.3932584269663, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=391, Percent_Identity=29.156010230179, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=391, Percent_Identity=29.156010230179, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.9684542586751, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=26.5350877192982, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=50.5307855626327, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=27.9411764705882, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=27.7161862527716, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=393, Percent_Identity=27.4809160305344, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=396, Percent_Identity=27.020202020202, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281365315, Length=396, Percent_Identity=27.020202020202, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17864292, Length=396, Percent_Identity=27.020202020202, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=26.5984654731458, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137564, Length=376, Percent_Identity=28.1914893617021, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645686, Length=457, Percent_Identity=27.5711159737418, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51427; Mature: 51296

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDTLFDKVWDLHKVSRLPGGSDQILIGLHLIHEVTSPQAFGALKDKNLKVKFPDRTVA
CCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLEKNCLEHKIRFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCDNKLSKGVF
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHH
AKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCHHHHCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKDKLCAPKNEYWDKALTWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSIS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEE
INQQIPLLDDLSPNDKLVAKEAYEYMSFKPGQYIKDTPVDVCFIGSCTNGRISDLRVAAR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VLKNKKVSKNVKAFVVPGSEKVATEAKQEGLDEIFKDSGFQWREPGCSMCLAMNSDKLIG
HHHCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFIN
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHC
>Mature Secondary Structure 
SQDTLFDKVWDLHKVSRLPGGSDQILIGLHLIHEVTSPQAFGALKDKNLKVKFPDRTVA
CCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLEKNCLEHKIRFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCDNKLSKGVF
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHH
AKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCHHHHCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKDKLCAPKNEYWDKALTWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSIS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEE
INQQIPLLDDLSPNDKLVAKEAYEYMSFKPGQYIKDTPVDVCFIGSCTNGRISDLRVAAR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VLKNKKVSKNVKAFVVPGSEKVATEAKQEGLDEIFKDSGFQWREPGCSMCLAMNSDKLIG
HHHCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFIN
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA