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The map label for this gene is leuD [H]

Identifier: 123967814

GI number: 123967814

Start: 254682

End: 255305

Strand: Reverse

Name: leuD [H]

Synonym: A9601_02771

Alternate gene names: 123967814

Gene position: 255305-254682 (Counterclockwise)

Preceding gene: 123967815

Following gene: 123967810

Centisome position: 15.29

GC content: 30.77

Gene sequence:

>624_bases
ATGAAATCAGAGTTTACCCCTCCAATTGGAAAAATTTCAAATATTAACGGGCAATGTATCCCATTGATTGGTAATGATAT
TGATACAGACCGAATAATTCCAGCTCGTTTTTTGAAGTGTGTTAACTTTGATTTATTGGGTGAATCTGTTTTTGCAGATG
ATAGAAAAAATTTAAAGGGAAGGCACCCTTTTGATCTAGAGGAAAACAAAAATGCCTCAATACTAGTTGTTAATAGCAAT
TTCGGATGTGGTTCCAGTAGAGAACATGCTCCCCAAGCACTTCTGAGATGGGGCATAAAAGCAATTATAGGCGAGAGTTT
CGCTGATATTTTTTATAGTAATTGTATAGCAATTGGAATTCCATGTTTTACGCTTACGAAAGAGTTAATACAAAAAATTC
AAAAACATAAAGATGATAAATTTTTATTTTTAGAAATTGATCTGAAAAAGTCCTTAGCAAAATCAAAAGATTTAAATTTC
AATCTTGAGATTAAGGAAAGCTCAAGAAAAATGTTTTTGTCAGGGGAATGGGATGCTACTTCAACACTGCTTGAGAATGA
AAATTTAATAGAAAATAAATTTACCCAATTACCTTATATAAAATTTAATACTAATTTGTTATGA

Upstream 100 bases:

>100_bases
AAGGATCTCCCAGTGGGAGAACACTGCTCATGAGTCCAGCAATGGTTGCTGCTGCTGCAATTAATGGCAAAGTTAGTGAC
GTTAGAGAATTTATCAACTG

Downstream 100 bases:

>100_bases
CTATTTAAATCCAGAGAGACTAAAAGAAATACCTCCAGCTTGATTATGAGGTTGATAAAAAATACCAACTTCATAGGATC
TTTTTTTCCAATTTAAAGAA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKSEFTPPIGKISNINGQCIPLIGNDIDTDRIIPARFLKCVNFDLLGESVFADDRKNLKGRHPFDLEENKNASILVVNSN
FGCGSSREHAPQALLRWGIKAIIGESFADIFYSNCIAIGIPCFTLTKELIQKIQKHKDDKFLFLEIDLKKSLAKSKDLNF
NLEIKESSRKMFLSGEWDATSTLLENENLIENKFTQLPYIKFNTNLL

Sequences:

>Translated_207_residues
MKSEFTPPIGKISNINGQCIPLIGNDIDTDRIIPARFLKCVNFDLLGESVFADDRKNLKGRHPFDLEENKNASILVVNSN
FGCGSSREHAPQALLRWGIKAIIGESFADIFYSNCIAIGIPCFTLTKELIQKIQKHKDDKFLFLEIDLKKSLAKSKDLNF
NLEIKESSRKMFLSGEWDATSTLLENENLIENKFTQLPYIKFNTNLL
>Mature_207_residues
MKSEFTPPIGKISNINGQCIPLIGNDIDTDRIIPARFLKCVNFDLLGESVFADDRKNLKGRHPFDLEENKNASILVVNSN
FGCGSSREHAPQALLRWGIKAIIGESFADIFYSNCIAIGIPCFTLTKELIQKIQKHKDDKFLFLEIDLKKSLAKSKDLNF
NLEIKESSRKMFLSGEWDATSTLLENENLIENKFTQLPYIKFNTNLL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=181, Percent_Identity=33.1491712707182, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=125, Percent_Identity=40.8, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23487; Mature: 23487

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEFTPPIGKISNINGQCIPLIGNDIDTDRIIPARFLKCVNFDLLGESVFADDRKNLKG
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCHHHHCCHHHCCCHHCCCC
RHPFDLEENKNASILVVNSNFGCGSSREHAPQALLRWGIKAIIGESFADIFYSNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
PCFTLTKELIQKIQKHKDDKFLFLEIDLKKSLAKSKDLNFNLEIKESSRKMFLSGEWDAT
CHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCEEEEEECCCCEEEEECCCCCH
STLLENENLIENKFTQLPYIKFNTNLL
HHHHCCCCHHHHHHHHCCEEEECCCCC
>Mature Secondary Structure
MKSEFTPPIGKISNINGQCIPLIGNDIDTDRIIPARFLKCVNFDLLGESVFADDRKNLKG
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCHHHHCCHHHCCCHHCCCC
RHPFDLEENKNASILVVNSNFGCGSSREHAPQALLRWGIKAIIGESFADIFYSNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
PCFTLTKELIQKIQKHKDDKFLFLEIDLKKSLAKSKDLNFNLEIKESSRKMFLSGEWDAT
CHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCEEEEEECCCCEEEEECCCCCH
STLLENENLIENKFTQLPYIKFNTNLL
HHHHCCCCHHHHHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]