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The map label for this gene is pyrB [H]

Identifier: 123967791

GI number: 123967791

Start: 236198

End: 237214

Strand: Reverse

Name: pyrB [H]

Synonym: A9601_02541

Alternate gene names: 123967791

Gene position: 237214-236198 (Counterclockwise)

Preceding gene: 123967792

Following gene: 123967788

Centisome position: 14.21

GC content: 34.02

Gene sequence:

>1017_bases
ATGCAAATTTGGCCTCATAAACATATACACACACTAGCTAATTTTTCAATTAAAGATTATGAGTCAGTATTTGAATTAGC
TAATAGATTTGATGCACTAAAGAATGCAGGAACAAAAAAGATACCGGCTTTACAAGGGACTTTGGTAACTTCTTTATTTT
TTGAAGCAAGTACAAGAACAAAAAATAGTTTTGAGCTTGCAGCAAAAAGACTTTCTGCTGATGTCCAAACGTTTGCGCCA
TCCTCCAGCTCTTTAACAAAAGGCGAAACAATAATTGATACCGCTATAACTTATTCAGCTATGGGGGCGGATACATTAGT
TATCAGACATTCATCAAGTTACATAACCTTTGAAATCGCAAAAAAACTTGATGCAATAAATTCCAAGACATCGGTTCTTA
ATGCGGGAGATGGATTACATAGTCACCCCAGCCAAGGATTGCTTGACATCTATACATTGATAAAATTCTTTTCCCCACAA
ACATTGAATCCAGAGGTTTTAAATTCCAAAAAAATTTTAATAATTGGAGACGTAAATCATTCAAGGGTTGCGAGGTCAAA
TCTTTGGGCTTTAAGTGCATTCGGCGCGGATATAATCTTATGTGGTCCTAAGGCATTAATACCTGATGAATTTATCAATT
TTTTAAAAACCCCCGCGCCAAATCAAACAGAAGATCCTGTTAAATCAAGAGGTTCCATAACAATTTCTAGATCATTGGAA
GAATCAATAAAAACTGCAGATGCGATTATTGTTTTAAGACTCCAGAAAGAGAGAATGATGGAAAATTTACTAAGTAGCAT
TGATTCATATAGTTTGGATTATGGCTTAACCCCAGAGAAATTATCTTTAAATAATAAAGAAATTCCAATTCTACATCCTG
GTCCCATTAACAGAGATATTGAAATAAGCAGCAAAGTGGTAGATCGATATCCTAATTGCTTAATAAATAATCAAGTTGCA
AATGGAATCCCCATAAGAATGGCTTTGCTTTATCTATTACAAAAACACAACAAGTAA

Upstream 100 bases:

>100_bases
AAAATGGCGTTGGTATCTCAAAAATAGTAGGAGTGTAAGTAAAAGATTAAAAGGTGACAGAACACCTAAATTTCTATAAT
CATTTATCTTTTTAAACGTA

Downstream 100 bases:

>100_bases
ATTTATAGCAACTTAAGACTTTCAGTAAAGAAACCATAAAATAATCCCAATCTTAAAGAATCTAATAAAAGTACTAAAAA
TTTCTTTTTCTTTTCAAATC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQIWPHKHIHTLANFSIKDYESVFELANRFDALKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAKKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSPQ
TLNPEVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKALIPDEFINFLKTPAPNQTEDPVKSRGSITISRSLE
ESIKTADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNNKEIPILHPGPINRDIEISSKVVDRYPNCLINNQVA
NGIPIRMALLYLLQKHNK

Sequences:

>Translated_338_residues
MQIWPHKHIHTLANFSIKDYESVFELANRFDALKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAKKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSPQ
TLNPEVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKALIPDEFINFLKTPAPNQTEDPVKSRGSITISRSLE
ESIKTADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNNKEIPILHPGPINRDIEISSKVVDRYPNCLINNQVA
NGIPIRMALLYLLQKHNK
>Mature_338_residues
MQIWPHKHIHTLANFSIKDYESVFELANRFDALKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFAP
SSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIAKKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSPQ
TLNPEVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKALIPDEFINFLKTPAPNQTEDPVKSRGSITISRSLE
ESIKTADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNNKEIPILHPGPINRDIEISSKVVDRYPNCLINNQVA
NGIPIRMALLYLLQKHNK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=328, Percent_Identity=29.5731707317073, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI2367364, Length=333, Percent_Identity=30.03003003003, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790703, Length=307, Percent_Identity=26.7100977198697, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786469, Length=301, Percent_Identity=26.578073089701, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=30, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=332, Percent_Identity=26.8072289156627, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=30.5389221556886, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37402; Mature: 37402

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIWPHKHIHTLANFSIKDYESVFELANRFDALKNAGTKKIPALQGTLVTSLFFEASTRT
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
KNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEHHH
KKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSPQTLNPEVLNSKKILIIGDVNH
HHHHHCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCH
SRVARSNLWALSAFGADIILCGPKALIPDEFINFLKTPAPNQTEDPVKSRGSITISRSLE
HHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCEEEEHHHH
ESIKTADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNNKEIPILHPGPINRDI
HHHHHHCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCEEECCCCCCCCC
EISSKVVDRYPNCLINNQVANGIPIRMALLYLLQKHNK
CHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIWPHKHIHTLANFSIKDYESVFELANRFDALKNAGTKKIPALQGTLVTSLFFEASTRT
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
KNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTAITYSAMGADTLVIRHSSSYITFEIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEHHH
KKLDAINSKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSPQTLNPEVLNSKKILIIGDVNH
HHHHHCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCH
SRVARSNLWALSAFGADIILCGPKALIPDEFINFLKTPAPNQTEDPVKSRGSITISRSLE
HHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCEEEEHHHH
ESIKTADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNNKEIPILHPGPINRDI
HHHHHHCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCEEECCCCCCCCC
EISSKVVDRYPNCLINNQVANGIPIRMALLYLLQKHNK
CHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA