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The map label for this gene is eno [H]

Identifier: 123967763

GI number: 123967763

Start: 211249

End: 212541

Strand: Direct

Name: eno [H]

Synonym: A9601_02261

Alternate gene names: 123967763

Gene position: 211249-212541 (Clockwise)

Preceding gene: 123967752

Following gene: 123967766

Centisome position: 12.65

GC content: 36.5

Gene sequence:

>1293_bases
GTGAAAGAAACTATTGATTTTCTGATTGATACTGTTGAAGCAAGGCAAGTCCTTGATTCAAGAGGTAATCCAACTGTAGA
GGCAGAAGTATTTTTGGAATGTGGTGCAAGTGGTAGAGCAATTGTTCCCAGCGGAGCTAGTACTGGTGCTCATGAGGCAC
ATGAATTAAGAGATGGTGGTTCGAAATATATGGGGAAAGGCGTTTTGAATGCTGTTAATAAAATTCATGAGACAATATCG
CCGGCTTTATGTGGTTTGTCATCTTTAGATCAAACTGCAGTAGATAAATTAATGATTGAAATTGATGGAACTCCTAATAA
GTCTAACCTTGGAGCAAATTCAATCCTTGCAGTAAGTCTTGCGACTGCTAGAGCATCAGCAAATGCTTTAGACATTCCCC
TATATAGATATCTTGGAGATCCATTATCCAATCTTCTTCCAGTCCCATTGATGAATGTAATAAATGGTGGCGCTCATGCA
CCAAATAGTCTTGATTTTCAGGAATTTATGCTTGTTCCACATGGAGTTAATAATTTCAGTGAATCATTAAGAATGGGTAC
TGAAATTTTTCACTCATTAAAATCATTACTTGATCAAAAAGGTCTATCTACTGCTGTAGGCGATGAGGGTGGATTTGCCC
CGAATTTGTCATCAAGCGTAGAAGCAGGGGACTTATTATTAGAAGCAATTCAAAAAGCCGGATTTAAGCCTGGTGAGCAG
GTATCTTTAGCTTTAGATGCTGCTAGTACTGAATTTTATAGGGATGGTATTTATAAATATGAAGGTAAAAGTTTAAATAG
TTCTGAAATGATTTCATATCTTTCAAGATTAGTTTCTAATTATCCCATAGTTTCAATAGAGGACGGTTTAGCTGAGGATG
ATTGGGAGGGTTGGTCAGAATTAAACAAAGAATTAGGGAATAAAGTTCAGCTTGTAGGTGATGATTTATTCGTTACTAAT
ACAGAAAGATTACGGAAAGGGATTATAGAAAAATCTGCCAATTCAATCCTAATAAAGGTAAATCAAATTGGGACATTAAC
TGAAACTTTGGAAGCTATTGAGTTAGCTAAAATGTCTGGTTTCACAAGTGTTATAAGTCATAGAAGTGGTGAAACTGAAG
ATACAACAATCGCAGATTTATCTGTCGCCACTAGATCGGGTCAGATCAAGACTGGCTCTTTGAGCAGAAGTGAAAGGATT
GCAAAATATAATAGGCTTTTAAAAATTGAGGAGGAATTAGGAAATCAAGCAAGATTCGCTGGAGCTCTAGGTTTAGGTCC
TAAAAATATATAG

Upstream 100 bases:

>100_bases
CTCTTACAAAAATAAAAATTATAGTTGGTTAACAAATATATTTCAGATATTAGCCAAATAGTTCAGCTATTAATAGGATT
TAAATAGTAATAATTAAATT

Downstream 100 bases:

>100_bases
TTTTTTTAGCGCTTAAGTTTTTCTAAACGTGAGCCTTTTCTCATACTTAATTGGCACTTTATCCAATCAATACTTATTGG
TAGTGCAAAAAAGAGTGGCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKETIDFLIDTVEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSSLDQTAVDKLMIEIDGTPNKSNLGANSILAVSLATARASANALDIPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVNNFSESLRMGTEIFHSLKSLLDQKGLSTAVGDEGGFAPNLSSSVEAGDLLLEAIQKAGFKPGEQ
VSLALDAASTEFYRDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIIEKSANSILIKVNQIGTLTETLEAIELAKMSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI

Sequences:

>Translated_430_residues
MKETIDFLIDTVEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSSLDQTAVDKLMIEIDGTPNKSNLGANSILAVSLATARASANALDIPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVNNFSESLRMGTEIFHSLKSLLDQKGLSTAVGDEGGFAPNLSSSVEAGDLLLEAIQKAGFKPGEQ
VSLALDAASTEFYRDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIIEKSANSILIKVNQIGTLTETLEAIELAKMSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI
>Mature_430_residues
MKETIDFLIDTVEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSSLDQTAVDKLMIEIDGTPNKSNLGANSILAVSLATARASANALDIPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVNNFSESLRMGTEIFHSLKSLLDQKGLSTAVGDEGGFAPNLSSSVEAGDLLLEAIQKAGFKPGEQ
VSLALDAASTEFYRDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIIEKSANSILIKVNQIGTLTETLEAIELAKMSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=50.7009345794392, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=425, Percent_Identity=50.5882352941176, Blast_Score=410, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=49.8850574712644, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=49.8850574712644, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=44.1108545034642, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=25.9701492537313, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=25.9701492537313, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=25.9701492537313, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1789141, Length=415, Percent_Identity=56.8674698795181, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=49.8834498834499, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=49.8834498834499, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.8421052631579, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=51.1574074074074, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.5773672055427, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.5773672055427, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=50.3464203233256, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.462962962963, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=48.7179487179487, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=48.7179487179487, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=48.3796296296296, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=48.3796296296296, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=48.3796296296296, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=48.3796296296296, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46060; Mature: 46060

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETIDFLIDTVEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGG
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHCCC
SKYMGKGVLNAVNKIHETISPALCGLSSLDQTAVDKLMIEIDGTPNKSNLGANSILAVSL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
ATARASANALDIPLYRYLGDPLSNLLPVPLMNVINGGAHAPNSLDFQEFMLVPHGVNNFS
HHHHCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHH
ESLRMGTEIFHSLKSLLDQKGLSTAVGDEGGFAPNLSSSVEAGDLLLEAIQKAGFKPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VSLALDAASTEFYRDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSE
EEEEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LNKELGNKVQLVGDDLFVTNTERLRKGIIEKSANSILIKVNQIGTLTETLEAIELAKMSG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
FTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERIAKYNRLLKIEEELGNQARFA
HHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GALGLGPKNI
HHCCCCCCCC
>Mature Secondary Structure
MKETIDFLIDTVEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGG
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHCCC
SKYMGKGVLNAVNKIHETISPALCGLSSLDQTAVDKLMIEIDGTPNKSNLGANSILAVSL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
ATARASANALDIPLYRYLGDPLSNLLPVPLMNVINGGAHAPNSLDFQEFMLVPHGVNNFS
HHHHCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHH
ESLRMGTEIFHSLKSLLDQKGLSTAVGDEGGFAPNLSSSVEAGDLLLEAIQKAGFKPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VSLALDAASTEFYRDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSE
EEEEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LNKELGNKVQLVGDDLFVTNTERLRKGIIEKSANSILIKVNQIGTLTETLEAIELAKMSG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
FTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERIAKYNRLLKIEEELGNQARFA
HHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GALGLGPKNI
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA