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The map label for this gene is spoT [H]

Identifier: 123967746

GI number: 123967746

Start: 192737

End: 195046

Strand: Reverse

Name: spoT [H]

Synonym: A9601_02091

Alternate gene names: 123967746

Gene position: 195046-192737 (Counterclockwise)

Preceding gene: 123967748

Following gene: 123967744

Centisome position: 11.68

GC content: 33.94

Gene sequence:

>2310_bases
ATGTCCGAGGCAGCTGCAAATTCAAAAGAAAAAAACGAAATTGAAGTTTCCAAAACTATTTTGCCTGAAAATAAAAAATA
TGAAAGTGAATCTTTGAATTATCAAATAAACATTCCCGATTGGCTTCTTAAAGATATTCATAATTTTGAAAAATCAAATA
AAGAAAATGATCAGAATCAAAACCTTATAGTAAAGGCTTTTAAACTTGCTTATAAAGCTCATGATGGACAATTCCGTGCG
AGCGGCGAACCATACATTATCCACCCGGTTGCTGTTGCAAATCTCCTTAAAGAAATAGGCGCTAGTTCATCTGTTATTGC
TGCAGGCCTTTTACATGATGTAGTTGAAGATACTGGCATTGATTTATCTGAAATAGAAACAAATTTTGGATTAGAAGTAA
AAATACTTGTAGAGGGTGTAACAAAATTAGGCGGCATTCACTTTAACAATAGGACTGAAGCACAAGCTGAAAATCTTAGG
AAAATGTTTTTGGCTATGGCCAGCGATTTCAGAGTTGTCTTAGTTAAACTTGCAGATCGACTTCATAACATGAGAACAAT
TGAATGGCTAAATGATGAGAAAAAACTAAGAATAGCGAGAGAAACAAGAGAGATTTATGCACCATTAGCTAATCGACTAG
GAATAAACAGATTTAAATGGGAATTAGAAGATTTAGCTTTTAAATTCCTAGAGCCTAAAGAATATCTAGATCTTAAAGAT
CAAATCGCCGTTAAAAGAAGTGATAGAGAAAAAAGATTAAAAGTAACTTTGAATCTTATGAAGGAAAACTTGATTTCAGC
GGGTTTGAAAAATTTTGAAATAACAGGAAGGCCAAAACATCTTTATGGCATCTGGAGCAAAATGGAAAGACAACAAAAGC
ATTTTCACGAGATTTATGATGTTGCTGCCCTAAGAATTATCGTGGACAATTCAGATAGTTGTTATAGAGCTTTAGCAGTT
GTTCATGATACTTTCAAACCAATTCCAGGTAGATTTAAAGACTATATAGGATTACCAAAACCTAATGGATATCAGTCCTT
ACACACTTCTGTGATTGGAAGACATCGACCTATTGAAGTTCAAATTAGAACTACTTCGATGCATCAAATTGCTGAATATG
GTATTGCCGCTCATTGGCAATATAAAGAGGGTGGTTCTCCTGCTAAAAGTAATGCCGAGAGATTTAATTGGCTAAGACAA
TTAGTAGAATGGCAACAAGAAGGTAATGAAAGGGATCATAATGATTATTTAGCTTCAATTAAAGAAGATTTATTTGATGA
AGAAGTATTTGTAATCACTCCAAAAGGAGATGTTGTTGGTTTAAGGAAAGGATCTACCGCGATAGATTTCGCCTACAGAA
TCCATTCTGAAGTTGGAAACCACTGTAATGGAATAAGAATTAATGAAAAGCTTTCTCCATTATCTACAGCACTTCAAAAT
GGTGACTTCATAGAAATTTTAACAAGTAATAATGCTACTCCAAGCTTGGATTGGCTGAACTTTGTAGTTACGCCAACTGC
TAAAAATAGAATTCGCCAATGGTATAAGAAAAGCCATCGTGATGAAACGATTAAAAGAGGTAGAGATTTACTTGAAAAAG
AAGTAGGTAGAAACGGTTTTGAAGCATTACTTTCTAGTGAAGCCATGAAAAAAGTTGCAAATCGATGCAATTTAAAAACT
ACTGAAGACCTTCTTGCATCTCTTGGTTTTGGTGGTTTAACTTTGCATCAAGTATTAAACAGACTAAGAGAAGAAATAAA
ATTACAGACAGAAGATGTAAAAAATGATTCTGACTCTGAAATAGCAAAATCTCTTAAAAGTAATAATAATTTATCCACTA
ATCAATCTAATACAGCAGCTAAATCACCAATTTCCGGGATAGAAGGTCTTGATTACAGAATAGGTAAATGCTGTTCCCCA
CTCCCAGGCGAGGATATTATCGGAACTGTGTCGCTCGGCAACCATGGGATAACTATACATAGGGAAGATTGTGAAAATGT
AATACCAATTCCAATAGAGAGAAGATTACCTGTCGGTTGGAATCAAGATAATAAAACTGGCGATAATAAGTTTCCAATTC
AGCTACGAATAGAAGTAATTGATCGAGTTGGAGTTCTCAAAGATATTCTTATGCGGTTATCTGATAAAGGTATAAACGTT
AGTGATGCCAATGTTAAAACTGCTTATGGTAAACCAGCTATTATAAATCTTTGTGTAGGTCTTGAAAGTTATAATCAACT
TCACAAAACAATTGAACAAATTAAATCAATGGCAGATGTTTTAGATATTGCCAGAGTTGGACAAAGTTAA

Upstream 100 bases:

>100_bases
CAATAAGATTTTTAACTACAATAATTTTTTCTTTATTTTTTTCCATGATTTATAGCAAATTTTTCTAGACATAAATAAAA
TACATCTAAAGCAATATAAA

Downstream 100 bases:

>100_bases
TTTTAAAATCAGATCAATCTATCTTTTACTTTTTATCTTGTAGATAAAGAAAACAATACTGCCGCAAATCAAAGCTAATA
AAATACCTACACAAGATGAA

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, (ppGpp)ase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQINIPDWLLKDIHNFEKSNKENDQNQNLIVKAFKLAYKAHDGQFRA
SGEPYIIHPVAVANLLKEIGASSSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDFRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYLDLKD
QIAVKRSDREKRLKVTLNLMKENLISAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAV
VHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQ
LVEWQQEGNERDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALLSSEAMKKVANRCNLKT
TEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNNNLSTNQSNTAAKSPISGIEGLDYRIGKCCSP
LPGEDIIGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAYGKPAIINLCVGLESYNQLHKTIEQIKSMADVLDIARVGQS

Sequences:

>Translated_769_residues
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQINIPDWLLKDIHNFEKSNKENDQNQNLIVKAFKLAYKAHDGQFRA
SGEPYIIHPVAVANLLKEIGASSSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDFRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYLDLKD
QIAVKRSDREKRLKVTLNLMKENLISAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAV
VHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQ
LVEWQQEGNERDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALLSSEAMKKVANRCNLKT
TEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNNNLSTNQSNTAAKSPISGIEGLDYRIGKCCSP
LPGEDIIGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAYGKPAIINLCVGLESYNQLHKTIEQIKSMADVLDIARVGQS
>Mature_768_residues
SEAAANSKEKNEIEVSKTILPENKKYESESLNYQINIPDWLLKDIHNFEKSNKENDQNQNLIVKAFKLAYKAHDGQFRAS
GEPYIIHPVAVANLLKEIGASSSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRK
MFLAMASDFRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYLDLKDQ
IAVKRSDREKRLKVTLNLMKENLISAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAVV
HDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQL
VEWQQEGNERDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQNG
DFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALLSSEAMKKVANRCNLKTT
EDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNNNLSTNQSNTAAKSPISGIEGLDYRIGKCCSPL
PGEDIIGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINVS
DANVKTAYGKPAIINLCVGLESYNQLHKTIEQIKSMADVLDIARVGQS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=139, Percent_Identity=38.1294964028777, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1790082, Length=733, Percent_Identity=38.881309686221, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1789147, Length=698, Percent_Identity=34.5272206303725, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87095; Mature: 86964

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQINIPDWLLKDIHNFEKSNKENDQNQ
CCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCC
NLIVKAFKLAYKAHDGQFRASGEPYIIHPVAVANLLKEIGASSSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
DLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDFRVVLVKLADR
CHHHHHCCCCCEEEEHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYLDLKD
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCHH
QIAVKRSDREKRLKVTLNLMKENLISAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYD
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVDNSDSCYRALAVVHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEV
HHEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQLVEWQQEGNERDHNDYLASI
EEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCC
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
EALLSSEAMKKVANRCNLKTTEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCHHH
IAKSLKSNNNLSTNQSNTAAKSPISGIEGLDYRIGKCCSPLPGEDIIGTVSLGNHGITIH
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEE
REDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAYGKPAIINLCVGLESYNQLHKTIEQIKSMADVLDIARVGQS
CCCCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEAAANSKEKNEIEVSKTILPENKKYESESLNYQINIPDWLLKDIHNFEKSNKENDQNQ
CCCCCCCCCCCCEEHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCC
NLIVKAFKLAYKAHDGQFRASGEPYIIHPVAVANLLKEIGASSSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
DLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDFRVVLVKLADR
CHHHHHCCCCCEEEEHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYLDLKD
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCHH
QIAVKRSDREKRLKVTLNLMKENLISAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYD
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVDNSDSCYRALAVVHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEV
HHEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQLVEWQQEGNERDHNDYLASI
EEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCC
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
EALLSSEAMKKVANRCNLKTTEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCHHH
IAKSLKSNNNLSTNQSNTAAKSPISGIEGLDYRIGKCCSPLPGEDIIGTVSLGNHGITIH
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEE
REDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAYGKPAIINLCVGLESYNQLHKTIEQIKSMADVLDIARVGQS
CCCCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]