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The map label for this gene is trmE [H]

Identifier: 123967744

GI number: 123967744

Start: 190826

End: 192208

Strand: Reverse

Name: trmE [H]

Synonym: A9601_02071

Alternate gene names: 123967744

Gene position: 192208-190826 (Counterclockwise)

Preceding gene: 123967746

Following gene: 123967743

Centisome position: 11.51

GC content: 27.77

Gene sequence:

>1383_bases
ATGGATTCGATAGTTACTACAGAAGATACGATAGCCGCAATTGCTTCAGCTATAAGTATTGGGAAGGGAGGAGTTGCGAT
AATAAGAGTATCAGGGAAAGACGCAATAAATTCTTGCAAAAAGATTGTTCAAACTAAATCTAAATATGCATGGGAATCAC
ATAGAGTTTTTCGTGGTTTTATTCAGGAAAATAAACAAAATAAATTTATAGATGAGGTTTTAATTTTAGTGATGAAATCC
CCAAATAGCTTCACAGGAGAGGATGTAGTTGAACTTCATTGCCATGGCGGAATTATCATAGTAAATAAAGTTTTAAAGAT
ATTATTATCTAATAATTCTAGAGTTAGACTTGCAAACCCAGGAGAATTTAGTCAAAGAGCTTTTCTTAATGGGAAAATAG
ACCTTACTCAAGCCGAGTCGATTAATCAATTAATTAATGCAAGCAATGCCAGATCAGCAGAGTTAGCCTTTAGCGGGGTT
CAAGGAGAAATAAAGAAAAAAATTGATGATATTAAAAATGACCTTATAAATCAACTTTGCGAAATAGAAGCGAGAGTTGA
TTTTGAAGAAGACTTCACAGATTTTGATTACACCAAATATCTAAAAAACATTAAAAAAGTCAAAGAAAAAATAGAATTAC
TAATAGAAAATGCAAAAAGAAATTCATATATTCACAATGGAATATCCATTGCGCTTATAGGAAAAACAAATGTTGGCAAA
AGCTCTTTATTAAATTTGCTTGCGAAAAAAGAGAAAGCAATCGTAACTAATATTCCTGGAACAACTAGAGATGTTATTGA
AGTTAATTTAACTATTAATGATATTCCAATGAAAATAATTGATACTGCTGGCATAAGAGAAACTCATGAACAAATTGAAA
GTATTGGAATTAAAAAAAGTTTTGGGAAAATAAAAGAGTCAGATTTTATAATTTATATTTATAGTCTTGAAGAAGGATTT
AATGAAGAAGACAAAAAAATAATACAAGAAATTCCCAAAGAAAAATTAATTACTATTTTGGGCAATAAAAAAGATTTAAT
TGATTGCAAAAATATTAATTCAAATGAGTTAAAACACACAATTCTTATGAGTATTAAGAATAATGATGGTGAAAGATTAT
TAATAGACACAATTATAAAAAAATGCGGATTAAAACAAGTAGAAAATATCAATATATTTTTAAACGATAGACATCTAACA
AATTTGTCTGCTTGCTTATCTAATTTAAATGATACTGATGAAATAATTGAAAATAAATTACCATTTGATTTGTTATCAAT
TGAGCTGAGAGATGGAATTCAAAACTTATCTAAAATAACTGGTCAAGAATTAACAGAGGAACTTCTAGATAATATTTTTT
CTAAGTTTTGTATTGGTAAATAA

Upstream 100 bases:

>100_bases
TTTTCTTATAGGTAATATCTCTTTTAAATCTCATTCAGAAAGAAAATTTCAAAATTAAATTCTTGATAATCTTACTTAAT
TATCCATAACAAAGCGAGAG

Downstream 100 bases:

>100_bases
ATTTGAGTTAAATTACATAGTGATATATAGTTGATTCTCTAACTTCAGTTTAATTTTTATTAATTAATTATTTATTAGTT
TAGTGGATTTAAATACTCCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSNNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNARSAELAFSGV
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKHTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNDRHLT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Sequences:

>Translated_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSNNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNARSAELAFSGV
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKHTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNDRHLT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
>Mature_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSNNSRVRLANPGEFSQRAFLNGKIDLTQAESINQLINASNARSAELAFSGV
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKHTILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNDRHLT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=28.8793103448276, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=29.9559471365639, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=27.4193548387097, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=33.6225596529284, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=32.5, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=28.3549783549784, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=31.8091451292247, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=29.746835443038, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51630; Mature: 51630

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIIIVNKVLKILLSNNSRVRLANP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNARSAELAFSGVQGEIKKKIDDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHCCCHHH
FGKIKESDFIIYIYSLEEGFNEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKHT
HCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCCHHHEEE
ILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNDRHLTNLSACLSNLNDTDEIIENKL
EEEEEECCCCCEEHHHHHHHHCCCCEECCEEEEEECCCHHHHHHHHHCCCCHHHHHHCCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIIIVNKVLKILLSNNSRVRLANP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNARSAELAFSGVQGEIKKKIDDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETHEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHCCCHHH
FGKIKESDFIIYIYSLEEGFNEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKHT
HCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCCHHHEEE
ILMSIKNNDGERLLIDTIIKKCGLKQVENINIFLNDRHLTNLSACLSNLNDTDEIIENKL
EEEEEECCCCCEEHHHHHHHHCCCCEECCEEEEEECCCHHHHHHHHHCCCCHHHHHHCCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA