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The map label for this gene is menE [H]

Identifier: 123967729

GI number: 123967729

Start: 177459

End: 178661

Strand: Reverse

Name: menE [H]

Synonym: A9601_01921

Alternate gene names: 123967729

Gene position: 178661-177459 (Counterclockwise)

Preceding gene: 123967730

Following gene: 123967726

Centisome position: 10.7

GC content: 26.93

Gene sequence:

>1203_bases
ATGAAAAATAAAATTCATACTATTGAAGTTGAAAATAACGAAACTCAATCTGTTGAGAAAATTATTGAAAAAATTAGAGA
GAATAAAATTATTTATGTAAAAAACAAATTTTACCAAGACAAAGATGTATTAGATTCAATTACTGAAAATGGGCCAGCAA
TTATCTTAAACAGTAGTGGGAGTTCTGGAAAACCGAGACAATGTTTTCACCATTTTGATAATCTTAAATTATCTGCAGCT
ACATCAGGTAAATGGCTAATAGAGCAAGGATTTGAATTGCAAAACTGCTTAATTTTCAATACTTTACCCCTGAATCATAT
AAGTGGATTAATGCCAATTTTTAGAAGTCAAACTTGGGGATGTGATTGTATTAATATTTCTCCGAATTTGATAAAAAAAA
CTCGAGAACTTTTACTTTTTACAATTAATTTTAAAAAAAACAAAAAATACTTGATTACGTCATTAGTACCTACCCAATTA
CAAAGACTTTTAGCTCAAAAAGATGGAATTAGTTGGCTAAAAATTTTTGATCTAATTTGGGTAGGAGGAGCTTCAATTCC
AGATGAAACTGCAGAACAATGTATAAAAGAAAAAATAAAATTAGCACCTTGTTACGGCTCAACTGAAACAGCAGCAATGG
TTACGAGTTTAAAACCAAAAGAATTCTTAATGGGTTATAAAAATGTTGGAGAAATACTACCTGATACAAAAATAAGAATT
AACGCACAAGGATTAATCGAGATAAAATCAGCCAGAATTGGAATCGAAATAATAGACTCTTTAAAAACTGAAAATTTCAA
AAATAAAAATGGGTGGTGGCAAACAGGTGATTTAGGTGAAATTAATCAAATCAATAATTCTTTCTATTTAAACTTTGTCG
GAAGAAGTGATAATGCCTTTAATTCAGGAGGAGAAATCGTTTTCCCTGAAGTAATTGAATCAAGATTAAATGATTTCATT
ATGAAAGAAAATATCCCAATTAATAAATTCAATATTTCAAAAGTATCTAACAAATTATGGGGAAATAAGATTAAAGTAAT
AGTTGAATTTAGAGAACTTACAAATAATAAAAATATTGAAATTTCTTTAAATTTATTAAAAAAATTTTCTCAAAATTGGC
CTAAACATGAAAGGCCTGAAAAGTGGATTGTTAAAAACAAAAATAGCATTACAGAAAAAATAAAATATAAATTTAAAAAA
TAA

Upstream 100 bases:

>100_bases
AATTACAAGGACCAACTCCAAAAGTTCCTGGTTTAGCAATGAATAAATTCCCTAATTCGTTTCTATTTTTAAATGAAGCA
AAAAAGATATGGGATCAACT

Downstream 100 bases:

>100_bases
TAATTAGCATTCTTTTAAATTTGTACTCACATTAGAAGCCTCTATCCATCTTTCTAACTGATCGGGAAGTTCTATTTTAT
CTCTTGAATCAATGTCTAAA

Product: putative O-succinylbenzoic acid--CoA ligase (OSB-CoA synthetase)

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKNKIHTIEVENNETQSVEKIIEKIRENKIIYVKNKFYQDKDVLDSITENGPAIILNSSGSSGKPRQCFHHFDNLKLSAA
TSGKWLIEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCDCINISPNLIKKTRELLLFTINFKKNKKYLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASIPDETAEQCIKEKIKLAPCYGSTETAAMVTSLKPKEFLMGYKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTGDLGEINQINNSFYLNFVGRSDNAFNSGGEIVFPEVIESRLNDFI
MKENIPINKFNISKVSNKLWGNKIKVIVEFRELTNNKNIEISLNLLKKFSQNWPKHERPEKWIVKNKNSITEKIKYKFKK

Sequences:

>Translated_400_residues
MKNKIHTIEVENNETQSVEKIIEKIRENKIIYVKNKFYQDKDVLDSITENGPAIILNSSGSSGKPRQCFHHFDNLKLSAA
TSGKWLIEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCDCINISPNLIKKTRELLLFTINFKKNKKYLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASIPDETAEQCIKEKIKLAPCYGSTETAAMVTSLKPKEFLMGYKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTGDLGEINQINNSFYLNFVGRSDNAFNSGGEIVFPEVIESRLNDFI
MKENIPINKFNISKVSNKLWGNKIKVIVEFRELTNNKNIEISLNLLKKFSQNWPKHERPEKWIVKNKNSITEKIKYKFKK
>Mature_400_residues
MKNKIHTIEVENNETQSVEKIIEKIRENKIIYVKNKFYQDKDVLDSITENGPAIILNSSGSSGKPRQCFHHFDNLKLSAA
TSGKWLIEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWGCDCINISPNLIKKTRELLLFTINFKKNKKYLITSLVPTQL
QRLLAQKDGISWLKIFDLIWVGGASIPDETAEQCIKEKIKLAPCYGSTETAAMVTSLKPKEFLMGYKNVGEILPDTKIRI
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTGDLGEINQINNSFYLNFVGRSDNAFNSGGEIVFPEVIESRLNDFI
MKENIPINKFNISKVSNKLWGNKIKVIVEFRELTNNKNIEISLNLLKKFSQNWPKHERPEKWIVKNKNSITEKIKYKFKK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=331, Percent_Identity=26.2839879154079, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 45957; Mature: 45957

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIHTIEVENNETQSVEKIIEKIRENKIIYVKNKFYQDKDVLDSITENGPAIILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
SSGKPRQCFHHFDNLKLSAATSGKWLIEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWG
CCCCHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
CDCINISPNLIKKTRELLLFTINFKKNKKYLITSLVPTQLQRLLAQKDGISWLKIFDLIW
CEEEECCHHHHHHHHEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGGASIPDETAEQCIKEKIKLAPCYGSTETAAMVTSLKPKEFLMGYKNVGEILPDTKIRI
CCCCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHCCCHHHHHHCHHHHHHHCCCCEEEE
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTGDLGEINQINNSFYLNFVGRSDNAF
ECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHCCCEEEEEEEECCCCCC
NSGGEIVFPEVIESRLNDFIMKENIPINKFNISKVSNKLWGNKIKVIVEFRELTNNKNIE
CCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEEEEEEEEHHCCCCCEE
ISLNLLKKFSQNWPKHERPEKWIVKNKNSITEKIKYKFKK
EEHHHHHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MKNKIHTIEVENNETQSVEKIIEKIRENKIIYVKNKFYQDKDVLDSITENGPAIILNSSG
CCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
SSGKPRQCFHHFDNLKLSAATSGKWLIEQGFELQNCLIFNTLPLNHISGLMPIFRSQTWG
CCCCHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
CDCINISPNLIKKTRELLLFTINFKKNKKYLITSLVPTQLQRLLAQKDGISWLKIFDLIW
CEEEECCHHHHHHHHEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHH
VGGASIPDETAEQCIKEKIKLAPCYGSTETAAMVTSLKPKEFLMGYKNVGEILPDTKIRI
CCCCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHCCCHHHHHHCHHHHHHHCCCCEEEE
NAQGLIEIKSARIGIEIIDSLKTENFKNKNGWWQTGDLGEINQINNSFYLNFVGRSDNAF
ECCCEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHCCCEEEEEEEECCCCCC
NSGGEIVFPEVIESRLNDFIMKENIPINKFNISKVSNKLWGNKIKVIVEFRELTNNKNIE
CCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEEEEEEEEHHCCCCCEE
ISLNLLKKFSQNWPKHERPEKWIVKNKNSITEKIKYKFKK
EEHHHHHHHHHCCCCCCCCCHHEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA