Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 123967586

GI number: 123967586

Start: 52576

End: 54048

Strand: Direct

Name: gatB [H]

Synonym: A9601_00491

Alternate gene names: 123967586

Gene position: 52576-54048 (Clockwise)

Preceding gene: 123967584

Following gene: 123967588

Centisome position: 3.15

GC content: 32.52

Gene sequence:

>1473_bases
ATGAACAATTTGGAATCTTGGGAAGCTGTGATTGGTTTGGAAACTCATGTACAGCTTAATACGAAAAGTAAAATATTCAC
ATCTGCGTCAACAGCTTTTGGTGATGCACCTAATACCCATATAGATCCTGTAGTTTGTGGTTTACCAGGAACTCTTCCAG
TTCTGAATGAGACTGTTCTTGAGTATGCTGTAAAAACTTCGTTAGCATTAAATTTAAACGTTGCAGAACATTGTAAATTT
GATAGAAAACAATATTTTTATCCTGATCTTCCTAAAAATTATCAAATTTCACAATTTGATGAGCCACTAGCTGAAAATGG
TTGGTTAGAGGTTGAAATAATTGAAAAGGACAAAGAACCTTATATAAAAAAAATTGGTATAGAAAGGCTACATATGGAAG
AAGACGCCGGAAAACTAGTCCATTCAGGAAGTGATAGATTAGCTGGCTCTAAGTATTCTTTAGTTGATTACAATCGTGCC
GGAATAGCATTGTGTGAGATTGTAAGTAAACCAGATATTAGATCAGGTAAAGAGGCATCTGAATACGCTTCAGAGATTAG
AAGAACAGTTAGATATCTAGGGGTATCAGACGGAAATATGCAAGAGGGTTCATTACGCTGCGATGTCAATATTTCAGTAA
GAAAAGGACCTAATGCTCCTTTTGGTACCAAAGTGGAAATAAAAAATATGAATTCATTTTCTGCAATTCAAAAGGCTTGT
GATTATGAAATAGCAAGACAAATAGAAGTTTATGAAAATGGAGGAAAAATTTTCCAAGAAACAAGGTTATGGGATGAAGC
TAAGCAATTAACGAAAAGTATGAGATTAAAAGAAGGTAGTAGTGACTATAGATATTTTCCTGATCCTGACTTAGGTCCAA
TTGAAATAACAAAAGCCCAACAAGAAATATGGTTTAAAGAACTACCAGAATTACCTTCAAAGAAAAGATATAAATACGTA
AGTCAATTTGGGTTGTCTGCATATGATGCAAGGGTAATTTCTGATGAAATAAGCATGGCAAACTTTTTTGAAGAAACAGT
AGCAAATGGTGCTGAGGCTAAACTAGCTTCAAATTGGGTAACAAGTGACATTGTGGGCTATTTAAAATCAAACAAACTAA
GTTTTAGTGAATTAAAGCTTAGTCCTGAAAATCTTGCTGAAATGATTAACATGATTTCAAAAAATATAATTAGTGGAAAA
ATTGCAAAAGAAATTTTACCTGAACTTATTCAAAAAAATATTTCCCCGAAAAAATTAGTTGAAGAAAAAGGGTTGGCAAT
GATATCTGATTCTTCAAGTATTTCACCAATAATTGATGAACTTATAAATGAACATCCAAATGAAGTCCAAGCATTTAAAA
ATGGCAAAACTAAGTTACTTGGTTTTTTTGTTGGTCAACTTATGAAAAGAACAAAAGGTAAAGCTGACCCTAAACTTGCA
AATAAACTTCTTATGGAAAAATTAAATAGCTAA

Upstream 100 bases:

>100_bases
ATTTTTGGTTTCTTGTGCCATTTAAAAATTAATATTTTTATTTGTGTTTTGGTCCTCTTTTCCTTTATATCCAATAATAT
TAATAAAGAGACTTTTAATA

Downstream 100 bases:

>100_bases
AGCTTAAAGAAGCTCATTTATCGTATTGATCCATTTATTTTGATCATCCGAATTCTCTAAAATAATATCTGAGAATTTTC
TTTTTTCTTCAAAACTTAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKEPYIKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRSGKEASEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGKIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQQEIWFKELPELPSKKRYKYV
SQFGLSAYDARVISDEISMANFFEETVANGAEAKLASNWVTSDIVGYLKSNKLSFSELKLSPENLAEMINMISKNIISGK
IAKEILPELIQKNISPKKLVEEKGLAMISDSSSISPIIDELINEHPNEVQAFKNGKTKLLGFFVGQLMKRTKGKADPKLA
NKLLMEKLNS

Sequences:

>Translated_490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKEPYIKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRSGKEASEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGKIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQQEIWFKELPELPSKKRYKYV
SQFGLSAYDARVISDEISMANFFEETVANGAEAKLASNWVTSDIVGYLKSNKLSFSELKLSPENLAEMINMISKNIISGK
IAKEILPELIQKNISPKKLVEEKGLAMISDSSSISPIIDELINEHPNEVQAFKNGKTKLLGFFVGQLMKRTKGKADPKLA
NKLLMEKLNS
>Mature_490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKEPYIKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRSGKEASEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGKIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQQEIWFKELPELPSKKRYKYV
SQFGLSAYDARVISDEISMANFFEETVANGAEAKLASNWVTSDIVGYLKSNKLSFSELKLSPENLAEMINMISKNIISGK
IAKEILPELIQKNISPKKLVEEKGLAMISDSSSISPIIDELINEHPNEVQAFKNGKTKLLGFFVGQLMKRTKGKADPKLA
NKLLMEKLNS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=37.6, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=352, Percent_Identity=37.7840909090909, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=518, Percent_Identity=31.4671814671815, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=34.0725806451613, Blast_Score=278, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54986; Mature: 54986

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVL
CCCHHHHHHHEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCEEEECCCCCCHHHHHHHH
EYAVKTSLALNLNVAEHCKFDRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKEP
HHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCH
YIKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRAGIALCEIVSKPDIRSGKEAS
HHHHHCHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHH
EYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHH
DYEIARQIEVYENGGKIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQ
HHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
QEIWFKELPELPSKKRYKYVSQFGLSAYDARVISDEISMANFFEETVANGAEAKLASNWV
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSDIVGYLKSNKLSFSELKLSPENLAEMINMISKNIISGKIAKEILPELIQKNISPKKLV
HHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EEKGLAMISDSSSISPIIDELINEHPNEVQAFKNGKTKLLGFFVGQLMKRTKGKADPKLA
HHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
NKLLMEKLNS
HHHHHHHHCC
>Mature Secondary Structure
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVL
CCCHHHHHHHEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCEEEECCCCCCHHHHHHHH
EYAVKTSLALNLNVAEHCKFDRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKEP
HHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCH
YIKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRAGIALCEIVSKPDIRSGKEAS
HHHHHCHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHH
EYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHH
DYEIARQIEVYENGGKIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQ
HHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
QEIWFKELPELPSKKRYKYVSQFGLSAYDARVISDEISMANFFEETVANGAEAKLASNWV
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSDIVGYLKSNKLSFSELKLSPENLAEMINMISKNIISGKIAKEILPELIQKNISPKKLV
HHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EEKGLAMISDSSSISPIIDELINEHPNEVQAFKNGKTKLLGFFVGQLMKRTKGKADPKLA
HHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
NKLLMEKLNS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA