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The map label for this gene is purF [H]

Identifier: 123967540

GI number: 123967540

Start: 4425

End: 5885

Strand: Direct

Name: purF [H]

Synonym: A9601_00031

Alternate gene names: 123967540

Gene position: 4425-5885 (Clockwise)

Preceding gene: 123967539

Following gene: 123967544

Centisome position: 0.26

GC content: 33.2

Gene sequence:

>1461_bases
ATGTGCGGAATAGTTGGAATCGTTTCTTCGAATGATGTAAATCAACAAATTTACGATAGTCTTTTGCTTCTGCAGCATAG
AGGTCAAGACTCAACAGGGATAGCAACAATGGAAAATACTGTTTTCCATATACATAAGGTTAAAGGTCAGGTTAATACTG
CTTATAGAACTAGAGATATGAGGAATTTAATTGGCAAAATTGGATTGGGTCATGTTAGGTATGCAACTAAGGGATCAGCA
GAAAGTGTAGAAGAAGCACAGCCTTTTTATGTTAATGCTCCTTATGGAATTGTTTTGATACATAACGGAAATTTGACTAA
TACCAGAGATTTAGAAAAACAGTTATTTAATGTGGACAAGCGGCATACAAATTCTTCAAGTGATACTGAAATGTTGTTAA
ATGTATTTGCGACAGAATTACAAGAACAAATTCATAATCAAGAATTAGAACCTGATATTATTTTTAGTGCGGTCAAATCT
TTACATAAAAGAATTCAGGGATCATATGCTTCAATTGCATTAATTTCAGGACATGGTTTATTGGCATTTAGAGATCCTTT
TGGGATAAGGCCTTTAGTCATAGGGAAAAGACTTTCCTTAACCACAAAAAAAGAAGAATGGATGGTTGCAAGCGAATCTT
TAGTACTTGAGAATAATGATTATCAAGTAGTGAGAGATGTAGATCCAGGAGAAGCTGTTTTTATAAATCTTGATGGGGAG
TTTTTCTCTAAGCAATGTTCTGATAATCCCATGCTATTTCCCTGTGCTTTTGAATATGTTTACTTAGCAAGGCCAGATTC
AATTATGAATGGAATTTCCGTTTATAAAGCTCGTTTAAAGATGGGAGATTATTTAGCAGAAACAATAAAAGAAACAATTA
ATTCTGGAGATATTGATGTAGTTATGCCTATTCCTGATTCTTCTCGACCTGCGGCAATGCAAGTTGCAAGACAGTTAGGG
ATAGAATATAGGGAAGGTTTTTTTAAAAATAGATATGTTGGCAGAACATTTATAATGCCTGGTCAGCAGAAACGTAAGAA
ATCTGTAAGGCAAAAATTAAATGCTATGAGTGCAGAGTTTAAAAATAAAAATGTATTAATTGTTGATGACTCGATAGTAA
GAGGTACTACTTCAAAAGAAATTGTGCAGATGGCTAAAGATGCAGGAGCAAACAAAGTTTTTTTTACATCAGCAGCTCCT
CCTGTTCGTTATCCTCACGTTTATGGAATTAATATGCCTAATAGAGATGAATTAATAGCACATAATAGGACAATAAGTGA
AATCGCCGATAAACTTGAAATTGATAATCTTGTTTATCAAAGTGTTGAAAGTTTGCGTAAATCTATAATTAGTGATTCTC
CTATTAAAGGTTTAGAGATGAGTTGTTTCACTGGTGATTATGTAACTGGAACAGTAAATCAAGAATACTTAAATTGGGTT
GAAAATGAATATAAATCTTAG

Upstream 100 bases:

>100_bases
GGGTTGAGGAATTAACCGAAAAATTTAATAATAGTATTTCAGATTACTTTTAAATATGAAAAATATTTCTCAACTATTTA
TCATTTTAAAATATTAAGTT

Downstream 100 bases:

>100_bases
TTGAGAAAGTTTTCAAGTCGGAAACAATTTTCAAGGTATTCATCATTATCTATTTTTAAAAAATCAATTAAAAAGTTTGA
TTTATTGGATTTGAAATTTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MCGIVGIVSSNDVNQQIYDSLLLLQHRGQDSTGIATMENTVFHIHKVKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNVDKRHTNSSSDTEMLLNVFATELQEQIHNQELEPDIIFSAVKS
LHKRIQGSYASIALISGHGLLAFRDPFGIRPLVIGKRLSLTTKKEEWMVASESLVLENNDYQVVRDVDPGEAVFINLDGE
FFSKQCSDNPMLFPCAFEYVYLARPDSIMNGISVYKARLKMGDYLAETIKETINSGDIDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSAEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRYPHVYGINMPNRDELIAHNRTISEIADKLEIDNLVYQSVESLRKSIISDSPIKGLEMSCFTGDYVTGTVNQEYLNWV
ENEYKS

Sequences:

>Translated_486_residues
MCGIVGIVSSNDVNQQIYDSLLLLQHRGQDSTGIATMENTVFHIHKVKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNVDKRHTNSSSDTEMLLNVFATELQEQIHNQELEPDIIFSAVKS
LHKRIQGSYASIALISGHGLLAFRDPFGIRPLVIGKRLSLTTKKEEWMVASESLVLENNDYQVVRDVDPGEAVFINLDGE
FFSKQCSDNPMLFPCAFEYVYLARPDSIMNGISVYKARLKMGDYLAETIKETINSGDIDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSAEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRYPHVYGINMPNRDELIAHNRTISEIADKLEIDNLVYQSVESLRKSIISDSPIKGLEMSCFTGDYVTGTVNQEYLNWV
ENEYKS
>Mature_486_residues
MCGIVGIVSSNDVNQQIYDSLLLLQHRGQDSTGIATMENTVFHIHKVKGQVNTAYRTRDMRNLIGKIGLGHVRYATKGSA
ESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNVDKRHTNSSSDTEMLLNVFATELQEQIHNQELEPDIIFSAVKS
LHKRIQGSYASIALISGHGLLAFRDPFGIRPLVIGKRLSLTTKKEEWMVASESLVLENNDYQVVRDVDPGEAVFINLDGE
FFSKQCSDNPMLFPCAFEYVYLARPDSIMNGISVYKARLKMGDYLAETIKETINSGDIDVVMPIPDSSRPAAMQVARQLG
IEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSAEFKNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAP
PVRYPHVYGINMPNRDELIAHNRTISEIADKLEIDNLVYQSVESLRKSIISDSPIKGLEMSCFTGDYVTGTVNQEYLNWV
ENEYKS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=37.8947368421053, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1788651, Length=483, Percent_Identity=53.8302277432712, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=29.4642857142857, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=489, Percent_Identity=33.3333333333333, Blast_Score=228, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=502, Percent_Identity=47.2111553784861, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI28573187, Length=508, Percent_Identity=34.4488188976378, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24659598, Length=508, Percent_Identity=34.6456692913386, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24659604, Length=483, Percent_Identity=35.4037267080745, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54658; Mature: 54658

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSSNDVNQQIYDSLLLLQHRGQDSTGIATMENTVFHIHKVKGQVNTAYRTRDM
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHH
RNLIGKIGLGHVRYATKGSAESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNVDK
HHHHHHCCCCEEEEECCCCHHHHHHCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCHH
RHTNSSSDTEMLLNVFATELQEQIHNQELEPDIIFSAVKSLHKRIQGSYASIALISGHGL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRLSLTTKKEEWMVASESLVLENNDYQVVRDVDPGEAVFINLDGE
EEECCCCCCCCEEECCEEEECCCCHHHEEECCEEEEECCCEEEEEECCCCCEEEEEECHH
FFSKQCSDNPMLFPCAFEYVYLARPDSIMNGISVYKARLKMGDYLAETIKETINSGDIDV
HHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VMPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSAEF
EEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
KNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAPPVRYPHVYGINMPNRDELIA
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHC
HNRTISEIADKLEIDNLVYQSVESLRKSIISDSPIKGLEMSCFTGDYVTGTVNQEYLNWV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
ENEYKS
HHHHCC
>Mature Secondary Structure
MCGIVGIVSSNDVNQQIYDSLLLLQHRGQDSTGIATMENTVFHIHKVKGQVNTAYRTRDM
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHH
RNLIGKIGLGHVRYATKGSAESVEEAQPFYVNAPYGIVLIHNGNLTNTRDLEKQLFNVDK
HHHHHHCCCCEEEEECCCCHHHHHHCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCHH
RHTNSSSDTEMLLNVFATELQEQIHNQELEPDIIFSAVKSLHKRIQGSYASIALISGHGL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRLSLTTKKEEWMVASESLVLENNDYQVVRDVDPGEAVFINLDGE
EEECCCCCCCCEEECCEEEECCCCHHHEEECCEEEEECCCEEEEEECCCCCEEEEEECHH
FFSKQCSDNPMLFPCAFEYVYLARPDSIMNGISVYKARLKMGDYLAETIKETINSGDIDV
HHHHHCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VMPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQQKRKKSVRQKLNAMSAEF
EEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
KNKNVLIVDDSIVRGTTSKEIVQMAKDAGANKVFFTSAAPPVRYPHVYGINMPNRDELIA
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHC
HNRTISEIADKLEIDNLVYQSVESLRKSIISDSPIKGLEMSCFTGDYVTGTVNQEYLNWV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
ENEYKS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]