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The map label for this gene is dnaN [H]

Identifier: 123967537

GI number: 123967537

Start: 168

End: 1325

Strand: Direct

Name: dnaN [H]

Synonym: A9601_00001

Alternate gene names: 123967537

Gene position: 168-1325 (Clockwise)

Preceding gene: NA

Following gene: 123967538

Centisome position: 0.01

GC content: 30.14

Gene sequence:

>1158_bases
ATGGAAATTATTTGTAATCAAAATGAATTAAATAATGCTATACAACTAGTAAGCAAGGCAGTTGCTTCAAGGCCAACGCA
TCCAATTCTTGCAAACATACTTTTAACAGCTGACGAAGGAACTAATAAAATTAGTGTCACAGGATTTGACTTAAATTTAG
GAATTCAAACTTCTTTTGATGGAACTGTCAAAAATAGTGGAGCTATCACTATACCCTCAAAACTTTTATCAGAAATAGTA
AACAAACTACCTAATGAAACTCCTGTTTCTCTAGAAGTAGACGAAAATTCAGATAATATTCTAATAAAAAGTGATAGAGG
TTCTTTTAATCTAAAAGGGATACCCTCTGATGAATATCCTAATTTGCCATTTGTTGAAAGCGGTACTTCTTTGAATATTG
AGCCTAGTTCTTTTTTAAAGGCTTTAAAATCTACCATTTTTGCCAGTAGTAATGATGATTCAAAGCAACTACTCACAGGT
GTCAATTTTACTTTCAAACCAAATTATTTAGAGTCTGCTTCTACAGATGGCCATAGATTGGCTGTTGCCTTAATTGGTAA
GGAAGAACAAATTGAAAATAAAGAAAACTTATCTTCAAATGTTGATGATTTATCGGTAACTATCCCAACTAGATCATTAA
GAGAAATTGAAAAACTAGTATCTTTGAGAAGCTCAGAAAATTCAATTAAGCTTTTCTATGACAAAGGTCAAGTAGTATTT
ATATCTTCTAATCAAATAATTACTACGAGAACTTTAGAAGGTACTTATCCTAATTATTCACAATTAATTCCTGATTCTTT
TTCTAAAATTATAAATTTTAATACAAAAAAATTAATTGATTCATTAGAAAGAATTGCTGTTTTGGCAGATCAGCAAAGTA
GTGTTGTAAAGATTAAATTAGATGATACAGATTTAGCTTCAATCAGCGCAGATGCTCAAGATATTGGAAATGCAAATGAA
TCAATACCTGTTTCTTATTCAGGAGAAAATTTTGATATTGCATTTAATGTAAGATATTTGTTAGAAGGTTTAAAAGTTAT
TGCCTCTGAAAATGTACTTTTAAAGTGTAATATTGCAACTACTCCAGCTGTTTTTGTACCAGAAGATAATCTCAATTCTT
TTACGTATCTAGTTATGCCAGTGCAGGTTCGTTCTTAA

Upstream 100 bases:

>100_bases
TTATTTTTTTATTTATATTTTAGAAAAAGAGCAAAAATAAATTTATTGAATTTAATTTACGATAAAAGTATATTGTATTT
GTAAAAAGTTCCAAATTCTG

Downstream 100 bases:

>100_bases
CTTGAAATTACCTAAAGAAATTTTATTAAGTGAATTATTAAATTATATTGTTAAGGGCAATATGGTCCTTAATTATGGAA
ATGGTGAAAATGTTTGGATG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADEGTNKISVTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNETPVSLEVDENSDNILIKSDRGSFNLKGIPSDEYPNLPFVESGTSLNIEPSSFLKALKSTIFASSNDDSKQLLTG
VNFTFKPNYLESASTDGHRLAVALIGKEEQIENKENLSSNVDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGTYPNYSQLIPDSFSKIINFNTKKLIDSLERIAVLADQQSSVVKIKLDDTDLASISADAQDIGNANE
SIPVSYSGENFDIAFNVRYLLEGLKVIASENVLLKCNIATTPAVFVPEDNLNSFTYLVMPVQVRS

Sequences:

>Translated_385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADEGTNKISVTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNETPVSLEVDENSDNILIKSDRGSFNLKGIPSDEYPNLPFVESGTSLNIEPSSFLKALKSTIFASSNDDSKQLLTG
VNFTFKPNYLESASTDGHRLAVALIGKEEQIENKENLSSNVDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGTYPNYSQLIPDSFSKIINFNTKKLIDSLERIAVLADQQSSVVKIKLDDTDLASISADAQDIGNANE
SIPVSYSGENFDIAFNVRYLLEGLKVIASENVLLKCNIATTPAVFVPEDNLNSFTYLVMPVQVRS
>Mature_385_residues
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADEGTNKISVTGFDLNLGIQTSFDGTVKNSGAITIPSKLLSEIV
NKLPNETPVSLEVDENSDNILIKSDRGSFNLKGIPSDEYPNLPFVESGTSLNIEPSSFLKALKSTIFASSNDDSKQLLTG
VNFTFKPNYLESASTDGHRLAVALIGKEEQIENKENLSSNVDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
ISSNQIITTRTLEGTYPNYSQLIPDSFSKIINFNTKKLIDSLERIAVLADQQSSVVKIKLDDTDLASISADAQDIGNANE
SIPVSYSGENFDIAFNVRYLLEGLKVIASENVLLKCNIATTPAVFVPEDNLNSFTYLVMPVQVRS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=384, Percent_Identity=25.5208333333333, Blast_Score=144, Evalue=9e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42102; Mature: 42102

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADEGTNKISVTGFDLNLGIQTSFD
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECEEECCC
GTVKNSGAITIPSKLLSEIVNKLPNETPVSLEVDENSDNILIKSDRGSFNLKGIPSDEYP
CCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEEECCCCCCCCC
NLPFVESGTSLNIEPSSFLKALKSTIFASSNDDSKQLLTGVNFTFKPNYLESASTDGHRL
CCCEEECCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCCHHCCCCCCCCEE
AVALIGKEEQIENKENLSSNVDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
EEEEECCHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
ISSNQIITTRTLEGTYPNYSQLIPDSFSKIINFNTKKLIDSLERIAVLADQQSSVVKIKL
EECCEEEEEEEECCCCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCEEEEEE
DDTDLASISADAQDIGNANESIPVSYSGENFDIAFNVRYLLEGLKVIASENVLLKCNIAT
CCCCCEECCCCHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCC
TPAVFVPEDNLNSFTYLVMPVQVRS
CCEEEEECCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MEIICNQNELNNAIQLVSKAVASRPTHPILANILLTADEGTNKISVTGFDLNLGIQTSFD
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECEEECCC
GTVKNSGAITIPSKLLSEIVNKLPNETPVSLEVDENSDNILIKSDRGSFNLKGIPSDEYP
CCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEEECCCCCCCCC
NLPFVESGTSLNIEPSSFLKALKSTIFASSNDDSKQLLTGVNFTFKPNYLESASTDGHRL
CCCEEECCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCCHHCCCCCCCCEE
AVALIGKEEQIENKENLSSNVDDLSVTIPTRSLREIEKLVSLRSSENSIKLFYDKGQVVF
EEEEECCHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCEEEE
ISSNQIITTRTLEGTYPNYSQLIPDSFSKIINFNTKKLIDSLERIAVLADQQSSVVKIKL
EECCEEEEEEEECCCCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCEEEEEE
DDTDLASISADAQDIGNANESIPVSYSGENFDIAFNVRYLLEGLKVIASENVLLKCNIAT
CCCCCEECCCCHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCC
TPAVFVPEDNLNSFTYLVMPVQVRS
CCEEEEECCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]