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The map label for this gene is lysS

Identifier: 123967040

GI number: 123967040

Start: 1591577

End: 1593115

Strand: Reverse

Name: lysS

Synonym: P9515_18071

Alternate gene names: 123967040

Gene position: 1593115-1591577 (Counterclockwise)

Preceding gene: 123967041

Following gene: 123967039

Centisome position: 93.48

GC content: 31.97

Gene sequence:

>1539_bases
TTGTCTGAAATAAGAGAAGCTCGCTTATTAAAAGCTAATTCACTAATTAATAAAGGATTTGAACCTTACGCAGAAACTTT
TAAGATATCTCATTCTACTAGATTCCTTAATGAGAAATTCGGCTATTTAGATAATGGTCAAGAGTTTGACCTAAACGTTT
CTCTTGCAGGAAGAGTTTTAGCAAAAAGAGTGATGGGGAAAATTGCTTTTTTTACCATTACTGATCAAGAAGGTAAGATT
CAGCTGTATTTAGAAAAAAGAATTATTGACGATTATGAAATCAATGCAAAACTGCTTTCTTTTGAAGATCTAAAGGAAAT
TGTAGATATTGGAGATTGGATAGGTGTTTTTGGAACTATTAAGAAAACTAATAAAGGTGAACTTTCAATCAAAGTCTCCA
AATGGGAAATGTTATCAAAATCATTACAGCCATTGCCTGATAAATGGCATGGTTTAACAGATATAGAAAAAAGATATAGG
CAAAGATATTTAGATCTAATAGTTAATCCTTTATCAAAAACTGTATTTAAAACAAGAGCAAAGTGTATTAGTCTGATAAG
AAGATGGTTAGATGAAAAAAATTTTTTAGAGATAGAGACACCAATACTTCAATCTGAGGCTGGAGGCGCTGAAGCCAGGC
CATTTATTACACATCATAATACTCTAGACATACCTCTTTATTTAAGAATAGCTACGGAATTGCATTTAAAAAGAATGGTC
GTAGGAGGGTTTGAGAAAGTATATGAGTTAGGACGTATTTTTCGTAATGAAGGCGTAAGTACAAAACATAATCCTGAATT
CACTTCAGTGGAAATTTATCAGGCTTTCTCTAATTACATAGATATGATGAATTTAACTGAAGATTTGATCAAAAATATTG
TGTTAAGTTGCTGTGATTCCTTAGTTATTAATTATCAAGATAAAGTAATTGATTTTTCAAAACCTTGGAAAAGAATATCT
ATGAAAAATGTTGTTAAGGAATATACCGGGATTGATTTTGACTCTTTTGATGGTGATTTGAATAAAGCTAAAAAGTTTTC
CGAGGAAATTAATATTGAAATATCGCCTAAAATAAATACCTTAGGTAGACTTCTAAATGAAGTTTTTGAACAAAAGGTTG
AATCTCAACTCATAGAACCAACTTTTGTGACTGATTATCCAATCGAAATATCCCCTCTAGCAAGACGCCATCCTAAAAAT
AAAGAGATGGTACAAAGATTTGAGTTGTTTATTGCGGGTCGTGAACTTGCTAACGCATTCAGTGAATTAATAGATCCAGT
TGATCAAAGACAAAGAATGCAATTACAGCAATCTTTGCGAGATGCAGGTGATCTTGAGGCTCATTGTATTGATGAAGATT
TTTTACAAGCTTTGGAAATAGGTATGCCACCCACTGGAGGTTTAGGAATTGGAATTGACAGGCTTGTTATGTTACTCACA
AATAGTTCCTCAATAAGAGATGTTATACCCTTCCCATTGTTAAAACCAGAACTAACTTCTAATAAATCTGAAAAATCAAC
CTCGAATGAAGTAAAATAA

Upstream 100 bases:

>100_bases
GTTATGCTTCTCAAAGAGTTGGAGAAACAGCAACATCTTTGGCAAGTTGATCCTAAGTCTGATAGTTTGATTAAATAAAA
CATAAATATTGAGTTAAATT

Downstream 100 bases:

>100_bases
AGATTGATCCAATACGAAATTTTTTAAATGAGTGGTGAACGTGTTGGTTTCCGCTTCAAGCACGCAGATGCAGTAGTGAA
AAGAAATCCTCAAGGCCGAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSEIREARLLKANSLINKGFEPYAETFKISHSTRFLNEKFGYLDNGQEFDLNVSLAGRVLAKRVMGKIAFFTITDQEGKI
QLYLEKRIIDDYEINAKLLSFEDLKEIVDIGDWIGVFGTIKKTNKGELSIKVSKWEMLSKSLQPLPDKWHGLTDIEKRYR
QRYLDLIVNPLSKTVFKTRAKCISLIRRWLDEKNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
VGGFEKVYELGRIFRNEGVSTKHNPEFTSVEIYQAFSNYIDMMNLTEDLIKNIVLSCCDSLVINYQDKVIDFSKPWKRIS
MKNVVKEYTGIDFDSFDGDLNKAKKFSEEINIEISPKINTLGRLLNEVFEQKVESQLIEPTFVTDYPIEISPLARRHPKN
KEMVQRFELFIAGRELANAFSELIDPVDQRQRMQLQQSLRDAGDLEAHCIDEDFLQALEIGMPPTGGLGIGIDRLVMLLT
NSSSIRDVIPFPLLKPELTSNKSEKSTSNEVK

Sequences:

>Translated_512_residues
MSEIREARLLKANSLINKGFEPYAETFKISHSTRFLNEKFGYLDNGQEFDLNVSLAGRVLAKRVMGKIAFFTITDQEGKI
QLYLEKRIIDDYEINAKLLSFEDLKEIVDIGDWIGVFGTIKKTNKGELSIKVSKWEMLSKSLQPLPDKWHGLTDIEKRYR
QRYLDLIVNPLSKTVFKTRAKCISLIRRWLDEKNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
VGGFEKVYELGRIFRNEGVSTKHNPEFTSVEIYQAFSNYIDMMNLTEDLIKNIVLSCCDSLVINYQDKVIDFSKPWKRIS
MKNVVKEYTGIDFDSFDGDLNKAKKFSEEINIEISPKINTLGRLLNEVFEQKVESQLIEPTFVTDYPIEISPLARRHPKN
KEMVQRFELFIAGRELANAFSELIDPVDQRQRMQLQQSLRDAGDLEAHCIDEDFLQALEIGMPPTGGLGIGIDRLVMLLT
NSSSIRDVIPFPLLKPELTSNKSEKSTSNEVK
>Mature_511_residues
SEIREARLLKANSLINKGFEPYAETFKISHSTRFLNEKFGYLDNGQEFDLNVSLAGRVLAKRVMGKIAFFTITDQEGKIQ
LYLEKRIIDDYEINAKLLSFEDLKEIVDIGDWIGVFGTIKKTNKGELSIKVSKWEMLSKSLQPLPDKWHGLTDIEKRYRQ
RYLDLIVNPLSKTVFKTRAKCISLIRRWLDEKNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVV
GGFEKVYELGRIFRNEGVSTKHNPEFTSVEIYQAFSNYIDMMNLTEDLIKNIVLSCCDSLVINYQDKVIDFSKPWKRISM
KNVVKEYTGIDFDSFDGDLNKAKKFSEEINIEISPKINTLGRLLNEVFEQKVESQLIEPTFVTDYPIEISPLARRHPKNK
EMVQRFELFIAGRELANAFSELIDPVDQRQRMQLQQSLRDAGDLEAHCIDEDFLQALEIGMPPTGGLGIGIDRLVMLLTN
SSSIRDVIPFPLLKPELTSNKSEKSTSNEVK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=496, Percent_Identity=40.9274193548387, Blast_Score=359, Evalue=4e-99,
Organism=Homo sapiens, GI194272210, Length=496, Percent_Identity=40.9274193548387, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI226491502, Length=337, Percent_Identity=24.3323442136499, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790571, Length=481, Percent_Identity=47.4012474012474, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789256, Length=480, Percent_Identity=46.875, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=30.2180685358255, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788173, Length=254, Percent_Identity=27.9527559055118, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787161, Length=363, Percent_Identity=21.763085399449, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=503, Percent_Identity=40.9542743538767, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=503, Percent_Identity=40.9542743538767, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=503, Percent_Identity=40.9542743538767, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551876, Length=351, Percent_Identity=23.0769230769231, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=244, Percent_Identity=25.4098360655738, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=509, Percent_Identity=40.6679764243615, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=507, Percent_Identity=31.163708086785, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=492, Percent_Identity=23.5772357723577, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=40.5940594059406, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=40.5940594059406, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136276, Length=474, Percent_Identity=23.8396624472574, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922416, Length=420, Percent_Identity=23.5714285714286, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_PROM5 (A2BZ03)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001012121.1
- ProteinModelPortal:   A2BZ03
- SMR:   A2BZ03
- STRING:   A2BZ03
- GeneID:   4719976
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_18071
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   FIAGHEI
- ProtClustDB:   PRK00484
- BioCyc:   PMAR167542:P9515ORF_1888-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 58873; Mature: 58741

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIREARLLKANSLINKGFEPYAETFKISHSTRFLNEKFGYLDNGQEFDLNVSLAGRVL
CCCHHHHHHHHHHHHHHCCCCHHHHHEECCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
AKRVMGKIAFFTITDQEGKIQLYLEKRIIDDYEINAKLLSFEDLKEIVDIGDWIGVFGTI
HHHHHHHEEEEEEECCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHH
KKTNKGELSIKVSKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPLSKTVFKTRA
CCCCCCCEEEEEHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCISLIRRWLDEKNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
HHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHH
VGGFEKVYELGRIFRNEGVSTKHNPEFTSVEIYQAFSNYIDMMNLTEDLIKNIVLSCCDS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVINYQDKVIDFSKPWKRISMKNVVKEYTGIDFDSFDGDLNKAKKFSEEINIEISPKINT
HHCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEECCCHHH
LGRLLNEVFEQKVESQLIEPTFVTDYPIEISPLARRHPKNKEMVQRFELFIAGRELANAF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SELIDPVDQRQRMQLQQSLRDAGDLEAHCIDEDFLQALEIGMPPTGGLGIGIDRLVMLLT
HHHHCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
NSSSIRDVIPFPLLKPELTSNKSEKSTSNEVK
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEIREARLLKANSLINKGFEPYAETFKISHSTRFLNEKFGYLDNGQEFDLNVSLAGRVL
CCHHHHHHHHHHHHHHCCCCHHHHHEECCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
AKRVMGKIAFFTITDQEGKIQLYLEKRIIDDYEINAKLLSFEDLKEIVDIGDWIGVFGTI
HHHHHHHEEEEEEECCCCCEEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHH
KKTNKGELSIKVSKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPLSKTVFKTRA
CCCCCCCEEEEEHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCISLIRRWLDEKNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
HHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHH
VGGFEKVYELGRIFRNEGVSTKHNPEFTSVEIYQAFSNYIDMMNLTEDLIKNIVLSCCDS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVINYQDKVIDFSKPWKRISMKNVVKEYTGIDFDSFDGDLNKAKKFSEEINIEISPKINT
HHCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEECCCHHH
LGRLLNEVFEQKVESQLIEPTFVTDYPIEISPLARRHPKNKEMVQRFELFIAGRELANAF
HHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SELIDPVDQRQRMQLQQSLRDAGDLEAHCIDEDFLQALEIGMPPTGGLGIGIDRLVMLLT
HHHHCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
NSSSIRDVIPFPLLKPELTSNKSEKSTSNEVK
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA