Click here to switch to the map view.

The map label for this gene is smpB [H]

Identifier: 123967038

GI number: 123967038

Start: 1590773

End: 1591270

Strand: Direct

Name: smpB [H]

Synonym: P9515_18051

Alternate gene names: 123967038

Gene position: 1590773-1591270 (Clockwise)

Preceding gene: 123967031

Following gene: 123967045

Centisome position: 93.35

GC content: 28.51

Gene sequence:

>498_bases
ATGATTGCAAAAGTTTCAAACAAATCAAATAAAAGCAACAAAAAAGAAGCCGTTTTTAAACGTCTTGCAGAAAATCGATA
TGCAAAATTTCAGTATGAAATCAGTGAAACAATCGAAGCTGGAATTGAACTTTTAGGGACTGAAGTGAAATCTATAAGAA
ACGGGAATGTAAATTTAAGAGATGGGTATTGTTCATTTAGAGATGGTGAAATACTTTTATTAAATGTCCACATTTCCCCA
CATAAAAATGTTGGCTCTTTTTTTAATCACGATCCACTACGAAATAGAAAATTATTGTTACATAAAAAAGAAATCTTTAA
GCTTAAATCCAATACTGAGAAAAAGGGGTTAACCATTATTCCCTTATCTATTTATTTAAAAGGGTCATGGATAAAATTAA
CAATTGGTATAGGTAAAGGTAAAAAATTACATGATAAAAGACAAGCCGATAAACAAAAAGATATTAAAAGAGAAATAAAA
ACTGCTCTTAAGAGATAA

Upstream 100 bases:

>100_bases
TCTTGAGCGAGGAATATTAGATTCATCTAAACTTGAAGAAATTATTGCCATAATTAAAGATTAATGGCAATTGTTCTTTG
GGGAAAGATTTTTTACAACT

Downstream 100 bases:

>100_bases
TTAAGGAACGACTTCTTTCAAATATTATTAATCAGAGTCTAAACTTACTGAATTGGGAGGAGGAGAAGGGTCCGGGTCAG
CAATTAACATGTGCTGTAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIAKVSNKSNKSNKKEAVFKRLAENRYAKFQYEISETIEAGIELLGTEVKSIRNGNVNLRDGYCSFRDGEILLLNVHISP
HKNVGSFFNHDPLRNRKLLLHKKEIFKLKSNTEKKGLTIIPLSIYLKGSWIKLTIGIGKGKKLHDKRQADKQKDIKREIK
TALKR

Sequences:

>Translated_165_residues
MIAKVSNKSNKSNKKEAVFKRLAENRYAKFQYEISETIEAGIELLGTEVKSIRNGNVNLRDGYCSFRDGEILLLNVHISP
HKNVGSFFNHDPLRNRKLLLHKKEIFKLKSNTEKKGLTIIPLSIYLKGSWIKLTIGIGKGKKLHDKRQADKQKDIKREIK
TALKR
>Mature_165_residues
MIAKVSNKSNKSNKKEAVFKRLAENRYAKFQYEISETIEAGIELLGTEVKSIRNGNVNLRDGYCSFRDGEILLLNVHISP
HKNVGSFFNHDPLRNRKLLLHKKEIFKLKSNTEKKGLTIIPLSIYLKGSWIKLTIGIGKGKKLHDKRQADKQKDIKREIK
TALKR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=135, Percent_Identity=41.4814814814815, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18970; Mature: 18970

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKVSNKSNKSNKKEAVFKRLAENRYAKFQYEISETIEAGIELLGTEVKSIRNGNVNLR
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEE
DGYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIFKLKSNTEKKGLTII
CCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCEEEE
PLSIYLKGSWIKLTIGIGKGKKLHDKRQADKQKDIKREIKTALKR
EEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAKVSNKSNKSNKKEAVFKRLAENRYAKFQYEISETIEAGIELLGTEVKSIRNGNVNLR
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEE
DGYCSFRDGEILLLNVHISPHKNVGSFFNHDPLRNRKLLLHKKEIFKLKSNTEKKGLTII
CCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCEEEE
PLSIYLKGSWIKLTIGIGKGKKLHDKRQADKQKDIKREIKTALKR
EEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]