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The map label for this gene is rlmN

Identifier: 123966894

GI number: 123966894

Start: 1450094

End: 1451140

Strand: Reverse

Name: rlmN

Synonym: P9515_16611

Alternate gene names: 123966894

Gene position: 1451140-1450094 (Counterclockwise)

Preceding gene: 123966895

Following gene: 123966893

Centisome position: 85.15

GC content: 31.81

Gene sequence:

>1047_bases
TTGAAAAACCTTCTAGGATGCAGTGTAAAGGATTTAGAAAATGTAGCTTTAAATTATGGACAAGCTGCGTTTAGAGGGCG
TCAAATTTATAGTTGGCTTTACAACTACAAAAACAGGTCTAAAAGTATTGATGAAATCAATGTCTTGCCATTAAATTTTA
GAAACCAATTAAAAAAGGAAGGTTTTATATTTGGGGAATTAATTTTAAAAGAAAAGTATTTAGCAAATGATGGAACCTTA
AAGTTGTTATTGAATACTAGAGATAATGAGAGTGTAGAGTGCGTTGGCATCCCTACTGAGAAAAGATTAACCGCATGTCT
TTCAAGTCAAGTGGGGTGCCCAATGGATTGCAAATTCTGTGCTACTGGTAAAGAAGGATTAAAAAGATCTTTAAAAGCAA
GTGAAATACTTGACCAAATTTTATTTATTGAAAATGAAATGAATCAGAAAGTAACTAATATTGTTTTTATGGGAATGGGT
GAACCTTTATTGAATATTGATGAGTTACTTTTTTCAATTAGATCAATAAATGAGGATTTTGATGTTAGTCAGCGAAGGAT
CACAGTAAGCACAGTTGCTATTCCAAACATGATCAGAAAACTATCAGAAATGTCTTTTCAAGTATTGGGTAAATGTCAGT
TTACGCTTGCAATAAGTTTGCATGCTTCAAATCAAAAAACAAGAGAAACTATAATACCTAGCGCAAAGAATTATCACATT
AAATATATTATTGATGATTGCAGAGAATTTGTAAAAAAGACTGGTAGAAGAGTTAGTTTCGAATACTTAATGCTTCATGG
GGTCAATGATAAATTAGAGCACGCTGATGAATTAAGTAATCTAATAAAAGGTTTTCAATGCCATGTCAATTTGATTCAAT
ATAATCAGATTGAGGAAGTTGAATTTAAGCAAACCCCTTCAAAGAATGCTCAACTCTTTCAAAACAGACTTTCTAATAAT
GGGATTAATGTCAGTCTTCGAAAAAGTAGAGGCTCAGACAGAAATGCGGCATGTGGTCAGTTAAGACAAAATGCCAAAAT
AAAATAA

Upstream 100 bases:

>100_bases
GGCGATGATTGGATTTATTGCTCTAATTCTGACTGAATTTTTCTTGAAACATGGATTATTACTATGGTAATTTTTCCAAA
TAATAAGGGTTAAGGGTAAC

Downstream 100 bases:

>100_bases
TCATTTTTAAAAACTTTTTATTAAATTCCACTCAAACACGTTATTATTGACTTAATTGTTTTTTTTTAATTGGGTTTCAT
AAGAACTAAACTGTTACCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKNLLGCSVKDLENVALNYGQAAFRGRQIYSWLYNYKNRSKSIDEINVLPLNFRNQLKKEGFIFGELILKEKYLANDGTL
KLLLNTRDNESVECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNQKVTNIVFMGMG
EPLLNIDELLFSIRSINEDFDVSQRRITVSTVAIPNMIRKLSEMSFQVLGKCQFTLAISLHASNQKTRETIIPSAKNYHI
KYIIDDCREFVKKTGRRVSFEYLMLHGVNDKLEHADELSNLIKGFQCHVNLIQYNQIEEVEFKQTPSKNAQLFQNRLSNN
GINVSLRKSRGSDRNAACGQLRQNAKIK

Sequences:

>Translated_348_residues
MKNLLGCSVKDLENVALNYGQAAFRGRQIYSWLYNYKNRSKSIDEINVLPLNFRNQLKKEGFIFGELILKEKYLANDGTL
KLLLNTRDNESVECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNQKVTNIVFMGMG
EPLLNIDELLFSIRSINEDFDVSQRRITVSTVAIPNMIRKLSEMSFQVLGKCQFTLAISLHASNQKTRETIIPSAKNYHI
KYIIDDCREFVKKTGRRVSFEYLMLHGVNDKLEHADELSNLIKGFQCHVNLIQYNQIEEVEFKQTPSKNAQLFQNRLSNN
GINVSLRKSRGSDRNAACGQLRQNAKIK
>Mature_348_residues
MKNLLGCSVKDLENVALNYGQAAFRGRQIYSWLYNYKNRSKSIDEINVLPLNFRNQLKKEGFIFGELILKEKYLANDGTL
KLLLNTRDNESVECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNQKVTNIVFMGMG
EPLLNIDELLFSIRSINEDFDVSQRRITVSTVAIPNMIRKLSEMSFQVLGKCQFTLAISLHASNQKTRETIIPSAKNYHI
KYIIDDCREFVKKTGRRVSFEYLMLHGVNDKLEHADELSNLIKGFQCHVNLIQYNQIEEVEFKQTPSKNAQLFQNRLSNN
GINVSLRKSRGSDRNAACGQLRQNAKIK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=34.4729344729345, Blast_Score=211, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PROM5 (A2BYK7)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011975.1
- STRING:   A2BYK7
- GeneID:   4720464
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_16611
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14457
- BioCyc:   PMAR167542:P9515ORF_1732-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39720; Mature: 39720

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLGCSVKDLENVALNYGQAAFRGRQIYSWLYNYKNRSKSIDEINVLPLNFRNQLKKE
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHC
GFIFGELILKEKYLANDGTLKLLLNTRDNESVECVGIPTEKRLTACLSSQVGCPMDCKFC
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIENEMNQKVTNIVFMGMGEPLLNIDELLFSIRSINEDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCC
DVSQRRITVSTVAIPNMIRKLSEMSFQVLGKCQFTLAISLHASNQKTRETIIPSAKNYHI
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHCCCCCCCEE
KYIIDDCREFVKKTGRRVSFEYLMLHGVNDKLEHADELSNLIKGFQCHVNLIQYNQIEEV
EEEHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
EFKQTPSKNAQLFQNRLSNNGINVSLRKSRGSDRNAACGQLRQNAKIK
HHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKNLLGCSVKDLENVALNYGQAAFRGRQIYSWLYNYKNRSKSIDEINVLPLNFRNQLKKE
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHC
GFIFGELILKEKYLANDGTLKLLLNTRDNESVECVGIPTEKRLTACLSSQVGCPMDCKFC
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIENEMNQKVTNIVFMGMGEPLLNIDELLFSIRSINEDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCC
DVSQRRITVSTVAIPNMIRKLSEMSFQVLGKCQFTLAISLHASNQKTRETIIPSAKNYHI
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHCCCCCCCEE
KYIIDDCREFVKKTGRRVSFEYLMLHGVNDKLEHADELSNLIKGFQCHVNLIQYNQIEEV
EEEHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
EFKQTPSKNAQLFQNRLSNNGINVSLRKSRGSDRNAACGQLRQNAKIK
HHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA