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The map label for this gene is truB

Identifier: 123966741

GI number: 123966741

Start: 1342775

End: 1343692

Strand: Direct

Name: truB

Synonym: P9515_15081

Alternate gene names: 123966741

Gene position: 1342775-1343692 (Clockwise)

Preceding gene: 123966739

Following gene: 123966743

Centisome position: 78.79

GC content: 27.89

Gene sequence:

>918_bases
ATGGAAATCAAAGATGGATTTATAATAATAAATAAAGAGAAAGGGTATACTTCTCATGATTGTGTTCAACAAATAAGAAA
ATTATTAGGTACAAAAAAAGTTGGGCATACAGGGACTTTAGATCCAGGAGTAACAGGTACATTACCAATTGCCATTGGAA
GTGCCACTAGATTTATTCAATACTTACCACAAGGAAAAACCTATATTGGGCAAATCCAATTAGGCATAAGAACAAAAACT
GATGATATACAAGGAGAAATCATTAATAAAAAAGAGTGGCCTATTTTGAGCAATGCTCAATTAGATAAATTTTTAAATAA
ATTTAGAGGGATAATTCAACAAATACCTCCAAAAGTATCTAGCGTTCACGTAAATGGAGAAAGAGCTTACAAAAAATTTT
TTAAGAATGAGGAATTCGAATTAAAACCAAGAGAAGTAAAAATAGAAGAATTAGTTCTGAAAAAATGGGACCAAATTAAT
GGAATATTAGAAATAAAAATTTCATGTTCAACAGGTACTTATATAAGATCTATAGCTAGAGATTTAGGGGGGGTATTAGA
CTCTGAAGGTTGTCTATTAAATCTTAAAAGAATTTCAGCTTGTGGATTCCATGAAAAAAATTCTATTAAAATATCTGATC
TAGTTAATCTGAACAAAAACTGTTCCACCTTTATTATTCCTACAATTTATGCACTTGATCACATTTCAACACTTATTTTA
AATAACCAAGAGGAAATTAATTTTTGGGAAACAGGAAGATTAATTAAATTAGATGAAGAAAATTTGATTAAAAGCTCCAA
ATTTGATTACAAAAAACCGATTAAAATTATCAATAATCAAAAAATGCTGTTAGGAATTGGTTTTATTAATGAAGATAAAA
ATAAATTACATCCCAAATTAGTTCTCAATGCAAAATAA

Upstream 100 bases:

>100_bases
AATATAAAATCATCTTTTTTCAATTTCTTGCCTAAATAATTCCTTATTTAAAAATAATAAAATAGTAAGAAATAGTTCAC
ATAGTAATCTTAATATTAAA

Downstream 100 bases:

>100_bases
TATTTATAAAAATTTTTTATTAAATGGTTCAATCCTTACACCCTTATAAAAATGCTTTAATATTCTTTCTGCTTTTATAC
CTTTGGAGGCCATAAAATTT

Product: putative tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEIKDGFIIINKEKGYTSHDCVQQIRKLLGTKKVGHTGTLDPGVTGTLPIAIGSATRFIQYLPQGKTYIGQIQLGIRTKT
DDIQGEIINKKEWPILSNAQLDKFLNKFRGIIQQIPPKVSSVHVNGERAYKKFFKNEEFELKPREVKIEELVLKKWDQIN
GILEIKISCSTGTYIRSIARDLGGVLDSEGCLLNLKRISACGFHEKNSIKISDLVNLNKNCSTFIIPTIYALDHISTLIL
NNQEEINFWETGRLIKLDEENLIKSSKFDYKKPIKIINNQKMLLGIGFINEDKNKLHPKLVLNAK

Sequences:

>Translated_305_residues
MEIKDGFIIINKEKGYTSHDCVQQIRKLLGTKKVGHTGTLDPGVTGTLPIAIGSATRFIQYLPQGKTYIGQIQLGIRTKT
DDIQGEIINKKEWPILSNAQLDKFLNKFRGIIQQIPPKVSSVHVNGERAYKKFFKNEEFELKPREVKIEELVLKKWDQIN
GILEIKISCSTGTYIRSIARDLGGVLDSEGCLLNLKRISACGFHEKNSIKISDLVNLNKNCSTFIIPTIYALDHISTLIL
NNQEEINFWETGRLIKLDEENLIKSSKFDYKKPIKIINNQKMLLGIGFINEDKNKLHPKLVLNAK
>Mature_305_residues
MEIKDGFIIINKEKGYTSHDCVQQIRKLLGTKKVGHTGTLDPGVTGTLPIAIGSATRFIQYLPQGKTYIGQIQLGIRTKT
DDIQGEIINKKEWPILSNAQLDKFLNKFRGIIQQIPPKVSSVHVNGERAYKKFFKNEEFELKPREVKIEELVLKKWDQIN
GILEIKISCSTGTYIRSIARDLGGVLDSEGCLLNLKRISACGFHEKNSIKISDLVNLNKNCSTFIIPTIYALDHISTLIL
NNQEEINFWETGRLIKLDEENLIKSSKFDYKKPIKIINNQKMLLGIGFINEDKNKLHPKLVLNAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=252, Percent_Identity=29.3650793650794, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI215599015, Length=242, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4503337, Length=242, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI2367200, Length=233, Percent_Identity=33.4763948497854, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17553978, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323204, Length=216, Percent_Identity=26.8518518518519, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=31.1557788944724, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROM5 (A2BY54)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011822.1
- ProteinModelPortal:   A2BY54
- SMR:   A2BY54
- STRING:   A2BY54
- GeneID:   4720072
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_15081
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- ProtClustDB:   CLSK2516628
- BioCyc:   PMAR167542:P9515ORF_1576-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34619; Mature: 34619

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDGFIIINKEKGYTSHDCVQQIRKLLGTKKVGHTGTLDPGVTGTLPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHH
YLPQGKTYIGQIQLGIRTKTDDIQGEIINKKEWPILSNAQLDKFLNKFRGIIQQIPPKVS
HCCCCCEEEEEEEEEEEECCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKFFKNEEFELKPREVKIEELVLKKWDQINGILEIKISCSTGTYIRSIAR
EEEECHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
DLGGVLDSEGCLLNLKRISACGFHEKNSIKISDLVNLNKNCSTFIIPTIYALDHISTLIL
HHCCCCCCCCCEEEEHHHHHCCCCCCCCEEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHC
NNQEEINFWETGRLIKLDEENLIKSSKFDYKKPIKIINNQKMLLGIGFINEDKNKLHPKL
CCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCHHHCCCCEEEEEEEEECCCCCCCCCEE
VLNAK
EEECC
>Mature Secondary Structure
MEIKDGFIIINKEKGYTSHDCVQQIRKLLGTKKVGHTGTLDPGVTGTLPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHH
YLPQGKTYIGQIQLGIRTKTDDIQGEIINKKEWPILSNAQLDKFLNKFRGIIQQIPPKVS
HCCCCCEEEEEEEEEEEECCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCCCE
SVHVNGERAYKKFFKNEEFELKPREVKIEELVLKKWDQINGILEIKISCSTGTYIRSIAR
EEEECHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
DLGGVLDSEGCLLNLKRISACGFHEKNSIKISDLVNLNKNCSTFIIPTIYALDHISTLIL
HHCCCCCCCCCEEEEHHHHHCCCCCCCCEEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHC
NNQEEINFWETGRLIKLDEENLIKSSKFDYKKPIKIINNQKMLLGIGFINEDKNKLHPKL
CCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCHHHCCCCEEEEEEEEECCCCCCCCCEE
VLNAK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA