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The map label for this gene is cobL

Identifier: 123966662

GI number: 123966662

Start: 1266985

End: 1268268

Strand: Direct

Name: cobL

Synonym: P9515_14291

Alternate gene names: NA

Gene position: 1266985-1268268 (Clockwise)

Preceding gene: 123966655

Following gene: 123966665

Centisome position: 74.35

GC content: 27.8

Gene sequence:

>1284_bases
ATGAGTCGGATAAAAAGAAAAATTCACGTAATAGGAATTAATTCTTTTGAATTTTTAGAGCTGCCCCTTAAATTACAAAA
ATTATTTAAAAAGACAGCTAATATTGCAGTTCCAAATACATATATAGGAGAAATAAAATTATGGGCAAAAAATAATACCA
ATAATAAAGAAAAATTATTTTTCGAAAGCAAAAGTGACAACAAACTAATTGATTGGCTTAAATCTCAAAAAACTGATGTA
ATTTTATTTTCTAGGGGTGATCCTCTTTGGTTTGGTATTGGGAGAATACTTCTAGAAAATTTTTCGAAAGAAGAATTAAG
TTTCTATCCGTCAAATACATGTGCTCAATTAGCATTTAGTAAACTCAAAATATCTTGGCAAGGGGCAGCATATGTAAGTA
TCCATGGAAGAGATTCAACCAAATTCATTAAGGCTATGAAATCAAAGCCTTCAAGTATAGCGGTCATTACAGATTCAAAT
AACTGCGGTTTAGAAACAATTCAGCATAATTTATTAGAATTAAATTTAGAAAATTATTATGACTTTTGGATTTGTGAGGA
AATAGGTTTTAAAAATGAAAGGATCAGAAAATTTAGCCTAAAAGAAAAGTTACCATTTGACATTTCTAATTTAAATATTG
CTGTTCTCCTGAAAAGAGAAAAAGATCTTCAAAGAGAAGCAACTCCTTTATTTGGATTAAAAGATGATACTTTTAAAACT
TTTGAAGATAGACCAAATTTATTAACCAAAAGGGAGATTCGAATTCAAATTTTAGCTGATTTAGAGTTACCAGAAAATGG
CGCAATTTGGGATATTGGAGCAGGTTGCGGCTCAATTGGTTTAGAAGCATTAAAATTAAGGCCCAATTTAAAATTATTTT
GTATTGACAAAAGAATTGGATCAAAAGAATTGATATTAGAAAATGCAAAAAGATTAGGCGTTAGACCTAAGGATATTTTT
GAAGAAGATATAAATAATTTAATTGATACTAATAACATTAAATCTTTTAAAAAAGCTAGGAGAATAATTATTGGTGGCTG
TGACAAAAATACTAAACTTCGAATTATTAATGAATTATCTAAAGATATGAATCATGGAGATATTGTTTCTATTCCTTTAA
TTGATATTCAAACCATTAAAGATTTAAGAGAGACTTTAAAAGTAAATAATTTCGAAGTAACTCTAAATCTTATTCAGACT
TATAAAAGTTTAAGTATTTCAGGGGGAATTAGAATGGATCCGAACAATCCTGTATTTTTGCTTAAAGGGAGAAAACTAAT
TTAA

Upstream 100 bases:

>100_bases
TCGCTTTCTCCTTTCTGACAAGTAATTTATTATTAATTTGTAAATACTAGGATAAATTGATTAAACACACAAATAAATTA
TATTATTCCTACTAAAATTC

Downstream 100 bases:

>100_bases
CTTAAAATTACTTATTAGGTTTACCCACATGAGGTAATCCCCAACCTAATTTATTTCTCAAAACTTGGAAAAATTCATGA
TCTTCAAGTCTTATAAACTT

Product: putative precorrin-6y methylase

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: Precorrin-6Y C5 15-Methyltransferase; Precorrin-6y Methylase; Precorrin-6Y C(5 15)-Methyltransferase; Precorrin-6Y Methylase; Precorrin-6y C5 15-Methyltransferase; Precorrin-6y C5 15-Methyltransferase Subunit CbiE; Decarboxylating Precorrin-6y C5 15-Methyltransferase; Precorrin-6y Methyltransferase; Precorrin-6Y C515-Methyltransferase CbiT Subunit; Cobalt-Precorrin-6Y C(5)-Methyltransferase; CbiET Protein; Precorrin-6Y C5 15-Methyltransferase Protein; Precorrin-6Y C515-Methyltransferase CbiE/T Subunits; Cobalt-Precorrin-6B C5 15-Methyltransferase; Precorrin-6B Methylase/Decarboxylase CbiT/CbiE; Precorrin-6y C(5 15)-Methyltransferase; Cobalamin(Vitamin; Precorrin-6Y C515-Methyltransferase Decarboxylating; Precorrin-6y C515-Methyltransferase Subunit CbiE; Cobalamin Biosynthesis Protein CbiET; Precorrin-6y C515-Methyltransferase CbiE/T Subunits; Precorrin-6Y C5 15-Methyltransferase Subunit CbiT; Decarboxylating Precorrin-6Y C5 15-Methyltransferase; Precorrin-6y-Dependent Methyltransferase; Precorrin-6y C5,15-Methyltransferase CbiE Subunit

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSRIKRKIHVIGINSFEFLELPLKLQKLFKKTANIAVPNTYIGEIKLWAKNNTNNKEKLFFESKSDNKLIDWLKSQKTDV
ILFSRGDPLWFGIGRILLENFSKEELSFYPSNTCAQLAFSKLKISWQGAAYVSIHGRDSTKFIKAMKSKPSSIAVITDSN
NCGLETIQHNLLELNLENYYDFWICEEIGFKNERIRKFSLKEKLPFDISNLNIAVLLKREKDLQREATPLFGLKDDTFKT
FEDRPNLLTKREIRIQILADLELPENGAIWDIGAGCGSIGLEALKLRPNLKLFCIDKRIGSKELILENAKRLGVRPKDIF
EEDINNLIDTNNIKSFKKARRIIIGGCDKNTKLRIINELSKDMNHGDIVSIPLIDIQTIKDLRETLKVNNFEVTLNLIQT
YKSLSISGGIRMDPNNPVFLLKGRKLI

Sequences:

>Translated_427_residues
MSRIKRKIHVIGINSFEFLELPLKLQKLFKKTANIAVPNTYIGEIKLWAKNNTNNKEKLFFESKSDNKLIDWLKSQKTDV
ILFSRGDPLWFGIGRILLENFSKEELSFYPSNTCAQLAFSKLKISWQGAAYVSIHGRDSTKFIKAMKSKPSSIAVITDSN
NCGLETIQHNLLELNLENYYDFWICEEIGFKNERIRKFSLKEKLPFDISNLNIAVLLKREKDLQREATPLFGLKDDTFKT
FEDRPNLLTKREIRIQILADLELPENGAIWDIGAGCGSIGLEALKLRPNLKLFCIDKRIGSKELILENAKRLGVRPKDIF
EEDINNLIDTNNIKSFKKARRIIIGGCDKNTKLRIINELSKDMNHGDIVSIPLIDIQTIKDLRETLKVNNFEVTLNLIQT
YKSLSISGGIRMDPNNPVFLLKGRKLI
>Mature_426_residues
SRIKRKIHVIGINSFEFLELPLKLQKLFKKTANIAVPNTYIGEIKLWAKNNTNNKEKLFFESKSDNKLIDWLKSQKTDVI
LFSRGDPLWFGIGRILLENFSKEELSFYPSNTCAQLAFSKLKISWQGAAYVSIHGRDSTKFIKAMKSKPSSIAVITDSNN
CGLETIQHNLLELNLENYYDFWICEEIGFKNERIRKFSLKEKLPFDISNLNIAVLLKREKDLQREATPLFGLKDDTFKTF
EDRPNLLTKREIRIQILADLELPENGAIWDIGAGCGSIGLEALKLRPNLKLFCIDKRIGSKELILENAKRLGVRPKDIFE
EDINNLIDTNNIKSFKKARRIIIGGCDKNTKLRIINELSKDMNHGDIVSIPLIDIQTIKDLRETLKVNNFEVTLNLIQTY
KSLSISGGIRMDPNNPVFLLKGRKLI

Specific function: Unknown

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 48989; Mature: 48858

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKRKIHVIGINSFEFLELPLKLQKLFKKTANIAVPNTYIGEIKLWAKNNTNNKEKLF
CCCCCCEEEEEECCCHHEEECCHHHHHHHHHHCCEECCCCEEEEEEEEEECCCCCCEEEE
FESKSDNKLIDWLKSQKTDVILFSRGDPLWFGIGRILLENFSKEELSFYPSNTCAQLAFS
EECCCCCHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCHHHHEECCCCHHHHHHHH
KLKISWQGAAYVSIHGRDSTKFIKAMKSKPSSIAVITDSNNCGLETIQHNLLELNLENYY
HEEEEECCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHEEEECCCCE
DFWICEEIGFKNERIRKFSLKEKLPFDISNLNIAVLLKREKDLQREATPLFGLKDDTFKT
EEEEECCCCCCCHHHHEEHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCHHH
FEDRPNLLTKREIRIQILADLELPENGAIWDIGAGCGSIGLEALKLRPNLKLFCIDKRIG
HCCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCC
SKELILENAKRLGVRPKDIFEEDINNLIDTNNIKSFKKARRIIIGGCDKNTKLRIINELS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCEEEHHHHHH
KDMNHGDIVSIPLIDIQTIKDLRETLKVNNFEVTLNLIQTYKSLSISGGIRMDPNNPVFL
HCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEEEHHHHHHCEECCCEEECCCCCEEE
LKGRKLI
EECCCCC
>Mature Secondary Structure 
SRIKRKIHVIGINSFEFLELPLKLQKLFKKTANIAVPNTYIGEIKLWAKNNTNNKEKLF
CCCCCEEEEEECCCHHEEECCHHHHHHHHHHCCEECCCCEEEEEEEEEECCCCCCEEEE
FESKSDNKLIDWLKSQKTDVILFSRGDPLWFGIGRILLENFSKEELSFYPSNTCAQLAFS
EECCCCCHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCHHHHEECCCCHHHHHHHH
KLKISWQGAAYVSIHGRDSTKFIKAMKSKPSSIAVITDSNNCGLETIQHNLLELNLENYY
HEEEEECCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHEEEECCCCE
DFWICEEIGFKNERIRKFSLKEKLPFDISNLNIAVLLKREKDLQREATPLFGLKDDTFKT
EEEEECCCCCCCHHHHEEHHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCHHH
FEDRPNLLTKREIRIQILADLELPENGAIWDIGAGCGSIGLEALKLRPNLKLFCIDKRIG
HCCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCC
SKELILENAKRLGVRPKDIFEEDINNLIDTNNIKSFKKARRIIIGGCDKNTKLRIINELS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCEEEHHHHHH
KDMNHGDIVSIPLIDIQTIKDLRETLKVNNFEVTLNLIQTYKSLSISGGIRMDPNNPVFL
HCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEEEHHHHHHCEECCCEEECCCCCEEE
LKGRKLI
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA