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The map label for this gene is argF [H]

Identifier: 123966657

GI number: 123966657

Start: 1262148

End: 1263074

Strand: Reverse

Name: argF [H]

Synonym: P9515_14241

Alternate gene names: 123966657

Gene position: 1263074-1262148 (Counterclockwise)

Preceding gene: 123966658

Following gene: 123966656

Centisome position: 74.12

GC content: 32.04

Gene sequence:

>927_bases
ATGATAAAACCTATCAAGCTTGCTAACAAAAACTTTTTATCAAGCTTGGATCTTTCTACTGATGAAATTATCAATATTTT
AGATCTTGCAAAAAATTTTAAAAATAATAAATTAAATGTTGATTTAAAAAATAAAGTATTAGGTTTAATTTTTGATAAAT
CATCAACACGTACAAGAGTTAGTTTTCAAGTCGCAATGTCAAGGCTTGGCGGGACTACAATCGATCTCAATCCGACAACC
TCACAAATCGGAAGGGGAGAACTAATAAAAGATACTGCAAGAGTTCTAGGCAGATATTGCGATGTGATTGCAATTAGAAC
ATTTAGTCATTCAGATTTGGAGGAATACGCAAAGTGGTCAACAAAACCAGTTATTAATGCTCTTACAGATTTAGAACATC
CATGTCAGGCATTAGCTGATTATTTAACAATTCAAGAGGAATTCACGGATTTCAAAGATGTGGTTTTGACATTTATAGGT
GATGGTAATAATGTCGCAAATTCTCTTATTTTATGTGGCGCATTATTAGGCGTGGAAGTTAGAATTGCTTGTCCCAAAGG
TTATGAACCCAACTCTTTTGTGATCAATAAAGCATACGAAATATACAATAATAAGAATTTATTGAAAATCTCTGATGATC
CTAATACTGCTGTTTTAGGAGCGAATGTTCTTTATACAGATGTTTGGTCATCAATGGGAGAAGAAAAACAAAAGGAAGAG
AAAGATAAGGTTTTTAATGGATTCACTATTGATAGTGATTTAGTAAAGAAGGCTGATAAAGAAGCAATTATCCTTCATTG
CCTTCCTGCATATAGATCTAAAGAGATAACTGATGAAGTATTTGAAAGTAAAAAAAATAGAATTTTTGATCAAGCAGAAA
ATAGATTACATGCTCAACAAGCCCTTTTATCTTGTCTTCTCCACTAG

Upstream 100 bases:

>100_bases
ATCCTCCAAGAAGAAGTTATTGAAGGTGAGGACTTAAAAAATCTTCTCGCCGAATCTAAAATGCCTGCATAGTAGAATCT
AGACTTTATTAGAATTATTA

Downstream 100 bases:

>100_bases
GAATACTTGCAAACTAATCAATAAAAGGGTACAAATGTATTAGTGTATATTTGTTTTATGTTAAATACATCTCAAGATAA
TCTTACAGATGCTCAAAATG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIKPIKLANKNFLSSLDLSTDEIINILDLAKNFKNNKLNVDLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPTT
SQIGRGELIKDTARVLGRYCDVIAIRTFSHSDLEEYAKWSTKPVINALTDLEHPCQALADYLTIQEEFTDFKDVVLTFIG
DGNNVANSLILCGALLGVEVRIACPKGYEPNSFVINKAYEIYNNKNLLKISDDPNTAVLGANVLYTDVWSSMGEEKQKEE
KDKVFNGFTIDSDLVKKADKEAIILHCLPAYRSKEITDEVFESKKNRIFDQAENRLHAQQALLSCLLH

Sequences:

>Translated_308_residues
MIKPIKLANKNFLSSLDLSTDEIINILDLAKNFKNNKLNVDLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPTT
SQIGRGELIKDTARVLGRYCDVIAIRTFSHSDLEEYAKWSTKPVINALTDLEHPCQALADYLTIQEEFTDFKDVVLTFIG
DGNNVANSLILCGALLGVEVRIACPKGYEPNSFVINKAYEIYNNKNLLKISDDPNTAVLGANVLYTDVWSSMGEEKQKEE
KDKVFNGFTIDSDLVKKADKEAIILHCLPAYRSKEITDEVFESKKNRIFDQAENRLHAQQALLSCLLH
>Mature_308_residues
MIKPIKLANKNFLSSLDLSTDEIINILDLAKNFKNNKLNVDLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPTT
SQIGRGELIKDTARVLGRYCDVIAIRTFSHSDLEEYAKWSTKPVINALTDLEHPCQALADYLTIQEEFTDFKDVVLTFIG
DGNNVANSLILCGALLGVEVRIACPKGYEPNSFVINKAYEIYNNKNLLKISDDPNTAVLGANVLYTDVWSSMGEEKQKEE
KDKVFNGFTIDSDLVKKADKEAIILHCLPAYRSKEITDEVFESKKNRIFDQAENRLHAQQALLSCLLH

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=38.0645161290323, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=29.4871794871795, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=37.0030581039755, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=36.7781155015198, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI2367364, Length=324, Percent_Identity=29.320987654321, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI48994908, Length=344, Percent_Identity=24.4186046511628, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=334, Percent_Identity=27.5449101796407, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=314, Percent_Identity=34.7133757961783, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=274, Percent_Identity=31.021897810219, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=29.073482428115, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34539; Mature: 34539

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPIKLANKNFLSSLDLSTDEIINILDLAKNFKNNKLNVDLKNKVLGLIFDKSSTRTRV
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCEEEEEECCCCCCHHH
SFQVAMSRLGGTTIDLNPTTSQIGRGELIKDTARVLGRYCDVIAIRTFSHSDLEEYAKWS
HHHHHHHHHCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCC
TKPVINALTDLEHPCQALADYLTIQEEFTDFKDVVLTFIGDGNNVANSLILCGALLGVEV
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCEEE
RIACPKGYEPNSFVINKAYEIYNNKNLLKISDDPNTAVLGANVLYTDVWSSMGEEKQKEE
EEECCCCCCCCCEEEEHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHHHHHCHHHHHHH
KDKVFNGFTIDSDLVKKADKEAIILHCLPAYRSKEITDEVFESKKNRIFDQAENRLHAQQ
HHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSCLLH
HHHHHHCC
>Mature Secondary Structure
MIKPIKLANKNFLSSLDLSTDEIINILDLAKNFKNNKLNVDLKNKVLGLIFDKSSTRTRV
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCEEEEEECCCCCCHHH
SFQVAMSRLGGTTIDLNPTTSQIGRGELIKDTARVLGRYCDVIAIRTFSHSDLEEYAKWS
HHHHHHHHHCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCC
TKPVINALTDLEHPCQALADYLTIQEEFTDFKDVVLTFIGDGNNVANSLILCGALLGVEV
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCEEE
RIACPKGYEPNSFVINKAYEIYNNKNLLKISDDPNTAVLGANVLYTDVWSSMGEEKQKEE
EEECCCCCCCCCEEEEHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHHHHHCHHHHHHH
KDKVFNGFTIDSDLVKKADKEAIILHCLPAYRSKEITDEVFESKKNRIFDQAENRLHAQQ
HHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSCLLH
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]