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The map label for this gene is epsC [H]

Identifier: 123966605

GI number: 123966605

Start: 1206319

End: 1208205

Strand: Reverse

Name: epsC [H]

Synonym: P9515_13721

Alternate gene names: 123966605

Gene position: 1208205-1206319 (Counterclockwise)

Preceding gene: 123966606

Following gene: 123966600

Centisome position: 70.9

GC content: 26.18

Gene sequence:

>1887_bases
ATGAATAGATTTTTATTCTTAAAAATAAGTTACCTATTAAGGTATTTACTGAATTTAAAAATTAAGCATAGAAAAATTTA
CTTCATATGTAGTGATCTTATACTTTTGACTTTTTCATATATATCCTCATTATGGTTATTAGATTTAAGCTACAATATTA
GGTATAAATACTTTCTGTTTTTTCTTTTTTTTACTTCAATCTATTTGATTTTTGGAAATTATTACAAGCACCTTACTAGA
TATTCTTCGAGTGTCGGCTTTTATAAATTAGCAATTAAGAATACATTATTAATAATATCTATACAAATCATATTTTATTT
ATTAGGTTTTTATTCTTATACTTTTAAATTTTGGTTACTTTTATGTTTTTTAAGCTCTTCTTTTACAATCGCATTAAGAG
TTTTAGTAAGGGATTTAATAACTATTTTCAATATATCCCCTAGAAATAATGTTAGTAAAATTGCTATTTATGGAACTGGA
CAAAATGAAGTTCTACTAAGTCAGCAAATTCTTTTAACAGGATCGCATAATATTGTTGCATTTATTGATGAGAATCCGGA
GTTAGAAAACAGAGATATCAATGGGATTAAAGTAAAATCTTTAAAGCAAATATCAAATTTAGATTTTGATCAATTACTTG
TTTCAACTTTATCTATTAATTCCAATCAATGGTTGAATATTCTAAAACAAATAAAAATAATAAAGCCTAGAATTAGAATA
TTGAAAATTCCTTCAATTAATAGTATTGAAGATGGAAAAGACAAAATAGATTTTTTAAAACCTATATCTTTAGAAGACCT
ATTATTTCGTAAAATCGTTCCTCCTAAGGAAAATTTGCTTGGTCCTGGAATTAAAGATAAGGTTGCATTAGTTACTGGTG
CAGCTGGTTCTATTGGTAAAGAGCTAAGTTTACAAATACTTTCGTTAAACCCCTCAAAACTTGTTGTAGTTGATTTTAGT
GAGCCTAATCTTTTTGAGCTTAAAAATATATTAATTAAAAATAATTTTCAAAATAAAAAAATCTATTGTGAATTAGGCAG
TACTTCAGACTATAAATTTATGCAAAATATTTTTACAAAGCATAATCCTGATATATTGTTTCATGCTTCTGCTTACAAAC
ATGTAACTTTATTAGAAGAAAATCAAATAGAAGCTATTAAAAATAACATAAAATCTACTCTAAATGTTTGTAAACTTGCT
TATAAATTTAATACTAATAAACTTATTTTAATTTCTTCCGATAAGGCAGTAAGGCCTACAAGTATTATGGGAGTTACAAA
AAGGATATCTGAACTTATCGTAAGTAGCTATGCGCAAAAATGTAAAAACTCAATAGATAAAGAAAAATTAGTATTTTCTA
TGGTACGTTTTGGGAATGTTTTAGGTTCATCTGGGTCAGTAGTTCCTATTTTTCAAAAACAGATAGAAGATGGAGGGCCA
GTAACTGTTACAGATCCTAGTGTTACAAGATACTTTATGACTCTTTCTGAAGCAGCTTCTTTGGTTATTCAATCTTCTGT
CTTAGCAAAAGGGGAAGGAGAAGTTTTTCTATTAGATATGGGAAAACCTATGCAAATAAAAAAGCTTGCAGAACAAATGA
TACTTTTAAATGGTCTTAAAATTAAAAATGAAGAAAATCTTGATGGTGATATTGAAATTGTCTATAGAGGTTTAGGTTCT
GGTGAAAAACTTTACGAAGAATTATTGATAGATGGGAAATCGCAAAAGACAGATCATCCATTAATTTTTAAAGCAATTGA
GAAATCTTTAGATTTCGATGACTTAAAAGGTAAACTTGATAATTTATTTATACATTTAAAAAATAATGATAATAAAAAAA
TGATTGAAATTCTTTGTGAAATTATCCCCGAATGGTTAAGAGAATAA

Upstream 100 bases:

>100_bases
CTTGTCTTGTCTAATTATAATGTATTATTTAGAGAAAAAATATGCCTTGTCCTTTGAGGAAGCGATGAATAATAAGTTAA
CTTGAAATTTTTCTTTTGAA

Downstream 100 bases:

>100_bases
TTTATAAAAATTTTTATACCATTTCACAAATTCCCTTATTCCATTTTTTAATGAGGTATTTGGTTTGTAGTTAATTAAAT
CTTCTATATAAGAAGTATCA

Product: nucleotide-diphosphate-sugar epimerase, membrane associated

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MNRFLFLKISYLLRYLLNLKIKHRKIYFICSDLILLTFSYISSLWLLDLSYNIRYKYFLFFLFFTSIYLIFGNYYKHLTR
YSSSVGFYKLAIKNTLLIISIQIIFYLLGFYSYTFKFWLLLCFLSSSFTIALRVLVRDLITIFNISPRNNVSKIAIYGTG
QNEVLLSQQILLTGSHNIVAFIDENPELENRDINGIKVKSLKQISNLDFDQLLVSTLSINSNQWLNILKQIKIIKPRIRI
LKIPSINSIEDGKDKIDFLKPISLEDLLFRKIVPPKENLLGPGIKDKVALVTGAAGSIGKELSLQILSLNPSKLVVVDFS
EPNLFELKNILIKNNFQNKKIYCELGSTSDYKFMQNIFTKHNPDILFHASAYKHVTLLEENQIEAIKNNIKSTLNVCKLA
YKFNTNKLILISSDKAVRPTSIMGVTKRISELIVSSYAQKCKNSIDKEKLVFSMVRFGNVLGSSGSVVPIFQKQIEDGGP
VTVTDPSVTRYFMTLSEAASLVIQSSVLAKGEGEVFLLDMGKPMQIKKLAEQMILLNGLKIKNEENLDGDIEIVYRGLGS
GEKLYEELLIDGKSQKTDHPLIFKAIEKSLDFDDLKGKLDNLFIHLKNNDNKKMIEILCEIIPEWLRE

Sequences:

>Translated_628_residues
MNRFLFLKISYLLRYLLNLKIKHRKIYFICSDLILLTFSYISSLWLLDLSYNIRYKYFLFFLFFTSIYLIFGNYYKHLTR
YSSSVGFYKLAIKNTLLIISIQIIFYLLGFYSYTFKFWLLLCFLSSSFTIALRVLVRDLITIFNISPRNNVSKIAIYGTG
QNEVLLSQQILLTGSHNIVAFIDENPELENRDINGIKVKSLKQISNLDFDQLLVSTLSINSNQWLNILKQIKIIKPRIRI
LKIPSINSIEDGKDKIDFLKPISLEDLLFRKIVPPKENLLGPGIKDKVALVTGAAGSIGKELSLQILSLNPSKLVVVDFS
EPNLFELKNILIKNNFQNKKIYCELGSTSDYKFMQNIFTKHNPDILFHASAYKHVTLLEENQIEAIKNNIKSTLNVCKLA
YKFNTNKLILISSDKAVRPTSIMGVTKRISELIVSSYAQKCKNSIDKEKLVFSMVRFGNVLGSSGSVVPIFQKQIEDGGP
VTVTDPSVTRYFMTLSEAASLVIQSSVLAKGEGEVFLLDMGKPMQIKKLAEQMILLNGLKIKNEENLDGDIEIVYRGLGS
GEKLYEELLIDGKSQKTDHPLIFKAIEKSLDFDDLKGKLDNLFIHLKNNDNKKMIEILCEIIPEWLRE
>Mature_628_residues
MNRFLFLKISYLLRYLLNLKIKHRKIYFICSDLILLTFSYISSLWLLDLSYNIRYKYFLFFLFFTSIYLIFGNYYKHLTR
YSSSVGFYKLAIKNTLLIISIQIIFYLLGFYSYTFKFWLLLCFLSSSFTIALRVLVRDLITIFNISPRNNVSKIAIYGTG
QNEVLLSQQILLTGSHNIVAFIDENPELENRDINGIKVKSLKQISNLDFDQLLVSTLSINSNQWLNILKQIKIIKPRIRI
LKIPSINSIEDGKDKIDFLKPISLEDLLFRKIVPPKENLLGPGIKDKVALVTGAAGSIGKELSLQILSLNPSKLVVVDFS
EPNLFELKNILIKNNFQNKKIYCELGSTSDYKFMQNIFTKHNPDILFHASAYKHVTLLEENQIEAIKNNIKSTLNVCKLA
YKFNTNKLILISSDKAVRPTSIMGVTKRISELIVSSYAQKCKNSIDKEKLVFSMVRFGNVLGSSGSVVPIFQKQIEDGGP
VTVTDPSVTRYFMTLSEAASLVIQSSVLAKGEGEVFLLDMGKPMQIKKLAEQMILLNGLKIKNEENLDGDIEIVYRGLGS
GEKLYEELLIDGKSQKTDHPLIFKAIEKSLDFDDLKGKLDNLFIHLKNNDNKKMIEILCEIIPEWLRE

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 71833; Mature: 71833

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFLFLKISYLLRYLLNLKIKHRKIYFICSDLILLTFSYISSLWLLDLSYNIRYKYFLF
CCCEEEEEHHHHHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHEEEEEEECCCHHHHHHH
FLFFTSIYLIFGNYYKHLTRYSSSVGFYKLAIKNTLLIISIQIIFYLLGFYSYTFKFWLL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHH
LCFLSSSFTIALRVLVRDLITIFNISPRNNVSKIAIYGTGQNEVLLSQQILLTGSHNIVA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCEEE
FIDENPELENRDINGIKVKSLKQISNLDFDQLLVSTLSINSNQWLNILKQIKIIKPRIRI
EECCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
LKIPSINSIEDGKDKIDFLKPISLEDLLFRKIVPPKENLLGPGIKDKVALVTGAAGSIGK
EECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCCCC
ELSLQILSLNPSKLVVVDFSEPNLFELKNILIKNNFQNKKIYCELGSTSDYKFMQNIFTK
CEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHC
HNPDILFHASAYKHVTLLEENQIEAIKNNIKSTLNVCKLAYKFNTNKLILISSDKAVRPT
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCH
SIMGVTKRISELIVSSYAQKCKNSIDKEKLVFSMVRFGNVLGSSGSVVPIFQKQIEDGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCC
VTVTDPSVTRYFMTLSEAASLVIQSSVLAKGEGEVFLLDMGKPMQIKKLAEQMILLNGLK
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEE
IKNEENLDGDIEIVYRGLGSGEKLYEELLIDGKSQKTDHPLIFKAIEKSLDFDDLKGKLD
ECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCC
NLFIHLKNNDNKKMIEILCEIIPEWLRE
EEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRFLFLKISYLLRYLLNLKIKHRKIYFICSDLILLTFSYISSLWLLDLSYNIRYKYFLF
CCCEEEEEHHHHHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHEEEEEEECCCHHHHHHH
FLFFTSIYLIFGNYYKHLTRYSSSVGFYKLAIKNTLLIISIQIIFYLLGFYSYTFKFWLL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHH
LCFLSSSFTIALRVLVRDLITIFNISPRNNVSKIAIYGTGQNEVLLSQQILLTGSHNIVA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCEEE
FIDENPELENRDINGIKVKSLKQISNLDFDQLLVSTLSINSNQWLNILKQIKIIKPRIRI
EECCCCCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
LKIPSINSIEDGKDKIDFLKPISLEDLLFRKIVPPKENLLGPGIKDKVALVTGAAGSIGK
EECCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCCCC
ELSLQILSLNPSKLVVVDFSEPNLFELKNILIKNNFQNKKIYCELGSTSDYKFMQNIFTK
CEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHC
HNPDILFHASAYKHVTLLEENQIEAIKNNIKSTLNVCKLAYKFNTNKLILISSDKAVRPT
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCH
SIMGVTKRISELIVSSYAQKCKNSIDKEKLVFSMVRFGNVLGSSGSVVPIFQKQIEDGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCC
VTVTDPSVTRYFMTLSEAASLVIQSSVLAKGEGEVFLLDMGKPMQIKKLAEQMILLNGLK
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEE
IKNEENLDGDIEIVYRGLGSGEKLYEELLIDGKSQKTDHPLIFKAIEKSLDFDDLKGKLD
ECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCC
NLFIHLKNNDNKKMIEILCEIIPEWLRE
EEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]