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The map label for this gene is rfbB [C]

Identifier: 123966604

GI number: 123966604

Start: 1205293

End: 1206330

Strand: Direct

Name: rfbB [C]

Synonym: P9515_13711

Alternate gene names: 123966604

Gene position: 1205293-1206330 (Clockwise)

Preceding gene: 123966603

Following gene: 123966617

Centisome position: 70.73

GC content: 29.19

Gene sequence:

>1038_bases
ATGGAAGAAAAAATATTAGTCACTGGTTGTGCAGGTTTTATAGGATTTCATGTATGTAAACAATTAATCAAAAAGGGGTT
ATTTGTTATTGGATTAGACAATCTTAATAATTACTACGATATTAGTTTAAAGAGAGCCCGTTTAAAAGAAATAGAAAATT
TTTCAAAAAATAATATTAAGGGAGAATTCTTATTTATAAAAGCAGATTTGAAGGATGAAAAAATTTTAAAAAACATTTCT
AAAGTACACTTACCAAAAAAAGTTATTCACCTTGCCGCCCAAGCAGGTGTAAGACACTCAATAGAAAATCCAAGAGCATA
TATTAATTCAAATTTGGTTGGGTTTGGTAATGTTCTTGAGTTCTGCAAGGATAACCAAGTTGATCACTTGATTTATGCAA
GTAGCAGTTCTATTTATGGTGGTAATAAAAAAATTCCATTTTCTGAGAAGGATTTTGTTGACTATCCAGTAAGTCTCTAT
GCAGCAACAAAAAAATCTAATGAGCTAATGGCCCATTCCTATAGTCATCTTTTTAAACTACCCTCTACTGGAATAAGACT
TTTTACAGTTTATGGTCCTTGGGGTAGACCAGATATGGCTCCAATGATCTTTACAAAATCAATTCTCTCCTCAAAGCCAA
TAAAAATTTTTAATAACGGCGAAATGTTTAGAGATTTTACCTACATTGATGATGTTAGTGAAGCAATACTGAAGCTCCTT
CACCTACCACCAAAATATTTAAATGATGACAAAAATTTAAACTCTTCAGAATTACCTGAATTAACTCCACATAGGATTAT
AAATATTGGAAGTAGTAATCCAATAAACCTATTAGAATTTATAGATATTCTCGAGAGCGAGATTAATATAAAAGCAATAA
GAGTATTTGAAAAAATGCAATTAGGAGACGTTAAAAAAACATATGCTGATACTTCTTATATAGAAGATTTAATTAACTAC
AAACCAAATACCTCATTAAAAAATGGAATAAGGGAATTTGTGAAATGGTATAAAAATTTTTATAAATTATTCTCTTAA

Upstream 100 bases:

>100_bases
AACTCTAATTGAAATTCAGAGTGGAGATTATATTGGAGAGGATGATATTGTAAGGTTCAAAGACGATTACGGTAGAATAA
ATGATTAAAATAAGATTTTT

Downstream 100 bases:

>100_bases
CCATTCGGGGATAATTTCACAAAGAATTTCAATCATTTTTTTATTATCATTATTTTTTAAATGTATAAATAAATTATCAA
GTTTACCTTTTAAGTCATCG

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEEKILVTGCAGFIGFHVCKQLIKKGLFVIGLDNLNNYYDISLKRARLKEIENFSKNNIKGEFLFIKADLKDEKILKNIS
KVHLPKKVIHLAAQAGVRHSIENPRAYINSNLVGFGNVLEFCKDNQVDHLIYASSSSIYGGNKKIPFSEKDFVDYPVSLY
AATKKSNELMAHSYSHLFKLPSTGIRLFTVYGPWGRPDMAPMIFTKSILSSKPIKIFNNGEMFRDFTYIDDVSEAILKLL
HLPPKYLNDDKNLNSSELPELTPHRIINIGSSNPINLLEFIDILESEINIKAIRVFEKMQLGDVKKTYADTSYIEDLINY
KPNTSLKNGIREFVKWYKNFYKLFS

Sequences:

>Translated_345_residues
MEEKILVTGCAGFIGFHVCKQLIKKGLFVIGLDNLNNYYDISLKRARLKEIENFSKNNIKGEFLFIKADLKDEKILKNIS
KVHLPKKVIHLAAQAGVRHSIENPRAYINSNLVGFGNVLEFCKDNQVDHLIYASSSSIYGGNKKIPFSEKDFVDYPVSLY
AATKKSNELMAHSYSHLFKLPSTGIRLFTVYGPWGRPDMAPMIFTKSILSSKPIKIFNNGEMFRDFTYIDDVSEAILKLL
HLPPKYLNDDKNLNSSELPELTPHRIINIGSSNPINLLEFIDILESEINIKAIRVFEKMQLGDVKKTYADTSYIEDLINY
KPNTSLKNGIREFVKWYKNFYKLFS
>Mature_345_residues
MEEKILVTGCAGFIGFHVCKQLIKKGLFVIGLDNLNNYYDISLKRARLKEIENFSKNNIKGEFLFIKADLKDEKILKNIS
KVHLPKKVIHLAAQAGVRHSIENPRAYINSNLVGFGNVLEFCKDNQVDHLIYASSSSIYGGNKKIPFSEKDFVDYPVSLY
AATKKSNELMAHSYSHLFKLPSTGIRLFTVYGPWGRPDMAPMIFTKSILSSKPIKIFNNGEMFRDFTYIDDVSEAILKLL
HLPPKYLNDDKNLNSSELPELTPHRIINIGSSNPINLLEFIDILESEINIKAIRVFEKMQLGDVKKTYADTSYIEDLINY
KPNTSLKNGIREFVKWYKNFYKLFS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=23.6768802228412, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=23.6768802228412, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=23.6768802228412, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI7657641, Length=351, Percent_Identity=26.4957264957265, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI42516563, Length=346, Percent_Identity=22.8323699421965, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=24.0331491712707, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786974, Length=358, Percent_Identity=24.8603351955307, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994969, Length=364, Percent_Identity=24.4505494505494, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=24.4897959183673, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=22.3433242506812, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=245, Percent_Identity=28.9795918367347, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=22.7397260273973, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=286, Percent_Identity=20.979020979021, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=142, Percent_Identity=32.3943661971831, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19923002, Length=354, Percent_Identity=24.8587570621469, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=23.0320699708455, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39507; Mature: 39507

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKILVTGCAGFIGFHVCKQLIKKGLFVIGLDNLNNYYDISLKRARLKEIENFSKNNIK
CCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCC
GEFLFIKADLKDEKILKNISKVHLPKKVIHLAAQAGVRHSIENPRAYINSNLVGFGNVLE
EEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHH
FCKDNQVDHLIYASSSSIYGGNKKIPFSEKDFVDYPVSLYAATKKSNELMAHSYSHLFKL
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCC
PSTGIRLFTVYGPWGRPDMAPMIFTKSILSSKPIKIFNNGEMFRDFTYIDDVSEAILKLL
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
HLPPKYLNDDKNLNSSELPELTPHRIINIGSSNPINLLEFIDILESEINIKAIRVFEKMQ
CCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LGDVKKTYADTSYIEDLINYKPNTSLKNGIREFVKWYKNFYKLFS
HCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEKILVTGCAGFIGFHVCKQLIKKGLFVIGLDNLNNYYDISLKRARLKEIENFSKNNIK
CCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCC
GEFLFIKADLKDEKILKNISKVHLPKKVIHLAAQAGVRHSIENPRAYINSNLVGFGNVLE
EEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHH
FCKDNQVDHLIYASSSSIYGGNKKIPFSEKDFVDYPVSLYAATKKSNELMAHSYSHLFKL
HHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCC
PSTGIRLFTVYGPWGRPDMAPMIFTKSILSSKPIKIFNNGEMFRDFTYIDDVSEAILKLL
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
HLPPKYLNDDKNLNSSELPELTPHRIINIGSSNPINLLEFIDILESEINIKAIRVFEKMQ
CCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LGDVKKTYADTSYIEDLINYKPNTSLKNGIREFVKWYKNFYKLFS
HCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]