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The map label for this gene is gmd [H]

Identifier: 123966533

GI number: 123966533

Start: 1139799

End: 1140767

Strand: Direct

Name: gmd [H]

Synonym: P9515_13001

Alternate gene names: 123966533

Gene position: 1139799-1140767 (Clockwise)

Preceding gene: 123966532

Following gene: 123966534

Centisome position: 66.88

GC content: 36.12

Gene sequence:

>969_bases
ATGACCAAGGCATTTATCACAGGTATAAATGGTCAGGACGGATCATATCTAGCAGAACACCTTTTATCACTTGGTTACGA
AGTAGGCGGACTTGTAAGAAGAAGCTCAGTTGCTGAAAATCAGACTTATAGAATTAATCCTATAGAAAGTAAGTTAAAAA
CATATTATGGCGATTTAATTGACCAGAATTCACTAGCAGATGCCTTTCGACATTTTAAACCTGATTTGATTTTTAATTTA
GCAGCTCAAAGTCATGTCAGACTTTCTTTTGATATGCCAGGTTTTACTGCACAAGTAAATGCAGTTGGTGCTTTAAATGT
TTTTACTGCGGCCAAGGAGAATTGTGAGGGTGCAAGAATCTATCAAGCCAGCTCCTCTGAAATGTTTGGAACTTCAGTTG
ACCCAGATAATTATCAACGAGAAACTACACCAATGCATCCCGTAAGTCCTTATGGATGTTCAAAATTATATGCATATAGT
ATTGCTAGAAATTTCCGAAGAGCCTATGGTCTTCATATTTCAAATGGCATCCTTTTTAATCACGAATCCCCAAGGAGAGG
GACAAATTTCGTGACAGCTAAGGTTGTAAAAGGTGCTCTTGACATTAAATACAAAAAAGAAAAATTCCTAGAATTAGGGA
ACCTAGATTCATACAGAGATTGGGGACATTCTTACGACTATACGAAAGCAATGATTAAATTATTAGAATATAGTGAACCT
TTGGATATCGTTATATCTACTGGGAAAGCCCACTCAGTCAAGGATCTATGTAAAGTGGTTTTTTCGAAACTTGGCATGGA
TTATGAAGAACATATAAAAATCAATCCTAAATTCCTCCGCCCTCAAGAACTTCCATACTTACAAGGAGATTCGACAAAAG
CAAAACAAATCCTTGGATGGGAACCGGAATTTACTTTTGAAACTTTAGTTGAAGATATGATTATGCATTTTGAGGCCTCT
TACAAATAA

Upstream 100 bases:

>100_bases
ATGAGATTAAAACTGGGCCTTATCACGGAGTTAAAAATGATAAAACATTTATATAAAATTCACCTCATTAACCTGTAAAA
TAAGATTTTCTTGTTGAATT

Downstream 100 bases:

>100_bases
TGAGAGAGCCTTTAAATCAATATGAACCTGATATTACAGAGGATGATATTGCAGCAGTTAATAAATACTTAAGGTCTGGA
GGTTACGTCACTGAGTTTAA

Product: putative GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTKAFITGINGQDGSYLAEHLLSLGYEVGGLVRRSSVAENQTYRINPIESKLKTYYGDLIDQNSLADAFRHFKPDLIFNL
AAQSHVRLSFDMPGFTAQVNAVGALNVFTAAKENCEGARIYQASSSEMFGTSVDPDNYQRETTPMHPVSPYGCSKLYAYS
IARNFRRAYGLHISNGILFNHESPRRGTNFVTAKVVKGALDIKYKKEKFLELGNLDSYRDWGHSYDYTKAMIKLLEYSEP
LDIVISTGKAHSVKDLCKVVFSKLGMDYEEHIKINPKFLRPQELPYLQGDSTKAKQILGWEPEFTFETLVEDMIMHFEAS
YK

Sequences:

>Translated_322_residues
MTKAFITGINGQDGSYLAEHLLSLGYEVGGLVRRSSVAENQTYRINPIESKLKTYYGDLIDQNSLADAFRHFKPDLIFNL
AAQSHVRLSFDMPGFTAQVNAVGALNVFTAAKENCEGARIYQASSSEMFGTSVDPDNYQRETTPMHPVSPYGCSKLYAYS
IARNFRRAYGLHISNGILFNHESPRRGTNFVTAKVVKGALDIKYKKEKFLELGNLDSYRDWGHSYDYTKAMIKLLEYSEP
LDIVISTGKAHSVKDLCKVVFSKLGMDYEEHIKINPKFLRPQELPYLQGDSTKAKQILGWEPEFTFETLVEDMIMHFEAS
YK
>Mature_321_residues
TKAFITGINGQDGSYLAEHLLSLGYEVGGLVRRSSVAENQTYRINPIESKLKTYYGDLIDQNSLADAFRHFKPDLIFNLA
AQSHVRLSFDMPGFTAQVNAVGALNVFTAAKENCEGARIYQASSSEMFGTSVDPDNYQRETTPMHPVSPYGCSKLYAYSI
ARNFRRAYGLHISNGILFNHESPRRGTNFVTAKVVKGALDIKYKKEKFLELGNLDSYRDWGHSYDYTKAMIKLLEYSEPL
DIVISTGKAHSVKDLCKVVFSKLGMDYEEHIKINPKFLRPQELPYLQGDSTKAKQILGWEPEFTFETLVEDMIMHFEASY
K

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=46.6076696165192, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI42516563, Length=323, Percent_Identity=23.2198142414861, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=41.2921348314607, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI48994969, Length=365, Percent_Identity=24.9315068493151, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=24.2937853107345, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=338, Percent_Identity=43.491124260355, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI133901788, Length=338, Percent_Identity=43.491124260355, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17507723, Length=338, Percent_Identity=43.1952662721894, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI133901786, Length=338, Percent_Identity=43.491124260355, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17539422, Length=338, Percent_Identity=43.491124260355, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI133901790, Length=338, Percent_Identity=43.491124260355, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24158427, Length=339, Percent_Identity=44.8377581120944, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=23.3846153846154, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36470; Mature: 36339

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAFITGINGQDGSYLAEHLLSLGYEVGGLVRRSSVAENQTYRINPIESKLKTYYGDLI
CCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHC
DQNSLADAFRHFKPDLIFNLAAQSHVRLSFDMPGFTAQVNAVGALNVFTAAKENCEGARI
CCHHHHHHHHHCCCHHEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCEE
YQASSSEMFGTSVDPDNYQRETTPMHPVSPYGCSKLYAYSIARNFRRAYGLHISNGILFN
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEC
HESPRRGTNFVTAKVVKGALDIKYKKEKFLELGNLDSYRDWGHSYDYTKAMIKLLEYSEP
CCCCCCCCCHHHHHHHHHHHCCEEHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCC
LDIVISTGKAHSVKDLCKVVFSKLGMDYEEHIKINPKFLRPQELPYLQGDSTKAKQILGW
EEEEEECCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCCCHHHHHCCC
EPEFTFETLVEDMIMHFEASYK
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKAFITGINGQDGSYLAEHLLSLGYEVGGLVRRSSVAENQTYRINPIESKLKTYYGDLI
CCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHC
DQNSLADAFRHFKPDLIFNLAAQSHVRLSFDMPGFTAQVNAVGALNVFTAAKENCEGARI
CCHHHHHHHHHCCCHHEEEECCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCEE
YQASSSEMFGTSVDPDNYQRETTPMHPVSPYGCSKLYAYSIARNFRRAYGLHISNGILFN
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEC
HESPRRGTNFVTAKVVKGALDIKYKKEKFLELGNLDSYRDWGHSYDYTKAMIKLLEYSEP
CCCCCCCCCHHHHHHHHHHHCCEEHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCC
LDIVISTGKAHSVKDLCKVVFSKLGMDYEEHIKINPKFLRPQELPYLQGDSTKAKQILGW
EEEEEECCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCCCCCCCCCCCCCCCHHHHHCCC
EPEFTFETLVEDMIMHFEASYK
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]