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The map label for this gene is ruvC

Identifier: 123966378

GI number: 123966378

Start: 997344

End: 997817

Strand: Reverse

Name: ruvC

Synonym: P9515_11451

Alternate gene names: 123966378

Gene position: 997817-997344 (Counterclockwise)

Preceding gene: 123966379

Following gene: 123966377

Centisome position: 58.55

GC content: 32.49

Gene sequence:

>474_bases
GTGAGGATTATTGGCATTGATCCTGGATTAGGAAGAGTTGGATATGGAATTATTGAAATACAAAATGAAAAGAAAAAATT
TCTTGATTGTGGCGTAATTGAAACTGATAAAAATAAAAAAGAAGAAGATAGACTTTATGAAATTTTTAATGATCTTAATA
CATTAATAAATCAATGGAAACCAGATATTGCAGCGGTCGAGAAGTTTTTCTTTTACAAATCGAGTACAACCATTAGTGTC
GTTCAGGCAAGAGGTGTAATTATGATGGTATTGGCTTCCAAAAATATTCAAGTTAGCGAGTATGCACCTTCTCAGGTCAA
ACTTACTATTGCAGGATCAGGTAAAGCATCAAAGAAAGAAGTTATTGAAGCTGTTATGTTTAACTTAAATTTGAATTATG
CACCAAAACCTGATGACTCCGCAGATGCACTGGCTATAGCTCTAACAAAATTAAATGAAGCTGGAATAAATTAG

Upstream 100 bases:

>100_bases
GATTCTGGTGAAAGGGTTATTCAAGCATTCTGTAAAGTCTTTGACTTTAACGAAAAAGATAACCTTTCTAAATTTCAATT
GTCATCAGAAGAATAATTAA

Downstream 100 bases:

>100_bases
ATTTAAATTATGATCTACTCTGAAAAAAAGAACTTTGAGAGGAGAAATTTTAAGAATTTAATGATAAAAAGTTCTTCTAT
TGATAGAAGAAATGTAGCAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIIGIDPGLGRVGYGIIEIQNEKKKFLDCGVIETDKNKKEEDRLYEIFNDLNTLINQWKPDIAAVEKFFFYKSSTTISV
VQARGVIMMVLASKNIQVSEYAPSQVKLTIAGSGKASKKEVIEAVMFNLNLNYAPKPDDSADALAIALTKLNEAGIN

Sequences:

>Translated_157_residues
MRIIGIDPGLGRVGYGIIEIQNEKKKFLDCGVIETDKNKKEEDRLYEIFNDLNTLINQWKPDIAAVEKFFFYKSSTTISV
VQARGVIMMVLASKNIQVSEYAPSQVKLTIAGSGKASKKEVIEAVMFNLNLNYAPKPDDSADALAIALTKLNEAGIN
>Mature_157_residues
MRIIGIDPGLGRVGYGIIEIQNEKKKFLDCGVIETDKNKKEEDRLYEIFNDLNTLINQWKPDIAAVEKFFFYKSSTTISV
VQARGVIMMVLASKNIQVSEYAPSQVKLTIAGSGKASKKEVIEAVMFNLNLNYAPKPDDSADALAIALTKLNEAGIN

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=35.5555555555556, Blast_Score=77, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PROM5 (A2BX41)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011459.1
- ProteinModelPortal:   A2BX41
- SMR:   A2BX41
- STRING:   A2BX41
- GeneID:   4719038
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_11451
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   PMAR167542:P9515ORF_1196-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17372; Mature: 17372

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGIDPGLGRVGYGIIEIQNEKKKFLDCGVIETDKNKKEEDRLYEIFNDLNTLINQWK
CEEEECCCCCCCCCEEEEEEECCCHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDIAAVEKFFFYKSSTTISVVQARGVIMMVLASKNIQVSEYAPSQVKLTIAGSGKASKKE
CCHHHHHHHHEECCCCEEEEEECCCEEEEEEECCCEEEECCCCCEEEEEEECCCCCHHHH
VIEAVMFNLNLNYAPKPDDSADALAIALTKLNEAGIN
HHHHHHHHCCCCCCCCCCCCCHHHHHHEEHHHCCCCC
>Mature Secondary Structure
MRIIGIDPGLGRVGYGIIEIQNEKKKFLDCGVIETDKNKKEEDRLYEIFNDLNTLINQWK
CEEEECCCCCCCCCEEEEEEECCCHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDIAAVEKFFFYKSSTTISVVQARGVIMMVLASKNIQVSEYAPSQVKLTIAGSGKASKKE
CCHHHHHHHHEECCCCEEEEEECCCEEEEEEECCCEEEECCCCCEEEEEEECCCCCHHHH
VIEAVMFNLNLNYAPKPDDSADALAIALTKLNEAGIN
HHHHHHHHCCCCCCCCCCCCCHHHHHHEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA