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The map label for this gene is spt [H]

Identifier: 123966235

GI number: 123966235

Start: 896220

End: 897404

Strand: Reverse

Name: spt [H]

Synonym: P9515_10021

Alternate gene names: 123966235

Gene position: 897404-896220 (Counterclockwise)

Preceding gene: 123966239

Following gene: 123966233

Centisome position: 52.66

GC content: 33.42

Gene sequence:

>1185_bases
TTGACTGAAACAAAACTCATTTCAACTATAAATGAAGAGAATTTACCTCTCCTGGGTAAAACTTACGTTCCTTCAAGACT
TTTATTAGGACCTGGTCCTTCAAATGCTCATCCTGAAGTTCTAAAGGCTTTATCACTTAATCCAATAGGGCATTTAGATG
ATGCTTACATAGAATTAATGTCTGATGTTCAAAAATTACTGAGATATACATGGCAGTGTAATAATCGAATAACTTTACCA
ATGAGTGGTACTGGCAGCGCAGCTATGGAAGCCTCGATAGCAAATTTTATTGAACCAGGTGAAAAAATTCTTATTGCAAG
AAAAGGATATTTTGGGGATCGTTTGGTAGATATGGCATCCAGATATAAAGCAGAAGTATCTGTAATTGATAAAGAATGGG
GTGAAGCTTTTACTTTTGAAGAGATTAAATATGAAATCGAAACAAATAAACCAGCCATTTTTGCTATTGTTCATGCTGAA
ACATCTACTGGAGTATTGCAACCTCTTGAAGGCATTGGAGATTTATGCAGAGAAAATAATTGCTTGTTCCTGGTTGATGC
AGTTACATCTTTAGGAGCGCTAGAACTTTATATTGATAAATGGAAAATTGACCTTGCTTATAGTTGCAGCCAAAAAGGTC
TAAGCTGCCCTCCTGGATTAAGCCCTTTTACAATGAATAAAAGAGCTGAAGATAAACTTAGTTCAAGATCTTCTAAAGTT
CCAAATTGGTATTTAGATTTATCTTTATTGAATAAATATTGGGGCTCTGATCGGGTTTATCACCACACAGCACCTGTTAA
TATGAATTTTGCAATAAGAGAGGGGTTGAGATTGATAGCCAATGAAGGTTTGAAAAATATATGGGAAAGACATAATTCTA
ATGCAATTAAATTATGGAAAGGTTTAGAATCTTTAGGAATGGAACTACATGTTTCTAGCGAAAATAGATTACCTACGCTT
ACAACAGTAAAAATTCCTTCTAGAGTTGACGGCGACGCATTTAGGAATCATTTATTAAAAAATTTTGGTATCGAGATAGG
AAATGGTTTAGGTTCTTTATCAGGCAAAGTATGGCGTATTGGTTTAATGGGATTTAATTCAAGTGATGAAAATGTTGATC
GATTATTAAATTTATTCGATACTGAATTAAAGAAATTTACTATTTTTGATTCCTCAACTTTTTAA

Upstream 100 bases:

>100_bases
TAATTGTTAGCTTAAATATTTAGATTGAATGAATTTCTTTAAACATTCATCGCTTTAATGGAGAATTTAGTTTAATTTAA
AAGTAACACTATAAAAGGTT

Downstream 100 bases:

>100_bases
ACCAAATCTTTATTCTATTTTCGCAATCCTTATCTAATATTCCCCGTATGACATTCATTTTATGATGAGCGCTTTCGTGT
TTAGATAGATCAATAGTACC

Product: serine:pyruvate/alanine:glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTETKLISTINEENLPLLGKTYVPSRLLLGPGPSNAHPEVLKALSLNPIGHLDDAYIELMSDVQKLLRYTWQCNNRITLP
MSGTGSAAMEASIANFIEPGEKILIARKGYFGDRLVDMASRYKAEVSVIDKEWGEAFTFEEIKYEIETNKPAIFAIVHAE
TSTGVLQPLEGIGDLCRENNCLFLVDAVTSLGALELYIDKWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRSSKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLKNIWERHNSNAIKLWKGLESLGMELHVSSENRLPTL
TTVKIPSRVDGDAFRNHLLKNFGIEIGNGLGSLSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFTIFDSSTF

Sequences:

>Translated_394_residues
MTETKLISTINEENLPLLGKTYVPSRLLLGPGPSNAHPEVLKALSLNPIGHLDDAYIELMSDVQKLLRYTWQCNNRITLP
MSGTGSAAMEASIANFIEPGEKILIARKGYFGDRLVDMASRYKAEVSVIDKEWGEAFTFEEIKYEIETNKPAIFAIVHAE
TSTGVLQPLEGIGDLCRENNCLFLVDAVTSLGALELYIDKWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRSSKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLKNIWERHNSNAIKLWKGLESLGMELHVSSENRLPTL
TTVKIPSRVDGDAFRNHLLKNFGIEIGNGLGSLSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFTIFDSSTF
>Mature_393_residues
TETKLISTINEENLPLLGKTYVPSRLLLGPGPSNAHPEVLKALSLNPIGHLDDAYIELMSDVQKLLRYTWQCNNRITLPM
SGTGSAAMEASIANFIEPGEKILIARKGYFGDRLVDMASRYKAEVSVIDKEWGEAFTFEEIKYEIETNKPAIFAIVHAET
STGVLQPLEGIGDLCRENNCLFLVDAVTSLGALELYIDKWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRSSKVP
NWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLKNIWERHNSNAIKLWKGLESLGMELHVSSENRLPTLT
TVKIPSRVDGDAFRNHLLKNFGIEIGNGLGSLSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFTIFDSSTF

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=388, Percent_Identity=40.7216494845361, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17536281, Length=371, Percent_Identity=40.7008086253369, Blast_Score=298, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6321079, Length=375, Percent_Identity=28.8, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=38.8586956521739, Blast_Score=297, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 44064; Mature: 43933

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETKLISTINEENLPLLGKTYVPSRLLLGPGPSNAHPEVLKALSLNPIGHLDDAYIELM
CCCCHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
SDVQKLLRYTWQCNNRITLPMSGTGSAAMEASIANFIEPGEKILIARKGYFGDRLVDMAS
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RYKAEVSVIDKEWGEAFTFEEIKYEIETNKPAIFAIVHAETSTGVLQPLEGIGDLCRENN
HHHHHHEEEEHHCCCCCCHHHEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCC
CLFLVDAVTSLGALELYIDKWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRSSKV
CEEEEECHHHCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLKNIWERHNSNAIKLWK
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
GLESLGMELHVSSENRLPTLTTVKIPSRVDGDAFRNHLLKNFGIEIGNGLGSLSGKVWRI
HHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCEEEE
GLMGFNSSDENVDRLLNLFDTELKKFTIFDSSTF
EEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCC
>Mature Secondary Structure 
TETKLISTINEENLPLLGKTYVPSRLLLGPGPSNAHPEVLKALSLNPIGHLDDAYIELM
CCCHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
SDVQKLLRYTWQCNNRITLPMSGTGSAAMEASIANFIEPGEKILIARKGYFGDRLVDMAS
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
RYKAEVSVIDKEWGEAFTFEEIKYEIETNKPAIFAIVHAETSTGVLQPLEGIGDLCRENN
HHHHHHEEEEHHCCCCCCHHHEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCC
CLFLVDAVTSLGALELYIDKWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRSSKV
CEEEEECHHHCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLKNIWERHNSNAIKLWK
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
GLESLGMELHVSSENRLPTLTTVKIPSRVDGDAFRNHLLKNFGIEIGNGLGSLSGKVWRI
HHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCEEEE
GLMGFNSSDENVDRLLNLFDTELKKFTIFDSSTF
EEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]