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The map label for this gene is dapF [H]

Identifier: 123966203

GI number: 123966203

Start: 864545

End: 865405

Strand: Reverse

Name: dapF [H]

Synonym: P9515_09701

Alternate gene names: 123966203

Gene position: 865405-864545 (Counterclockwise)

Preceding gene: 123966205

Following gene: 123966202

Centisome position: 50.78

GC content: 29.27

Gene sequence:

>861_bases
ATGAAAAATATAATTTTTGAAAAATATCATGGGAATGGTAATGATTTTATAATTATTGATACTAGAAACAAAAATATATT
CAATAAATTTATATCAGAAAATATTTTCGATATTAAAGAATTGTGTGATCGACAATTTGGTATAGGAGGAGATGGTTTGA
TTTTTATCTTGGAACCTAATGGAAATAATCACGCAAGGATGATTATCTATAATTCAGATGGTTCAGAAGCTCAAATGTGT
GGAAACGGAATTAGGTGTTTGGTTCAATATCTACATAATTCTAAGAAAGGATCTCATAACTCTTCTGAGTATCGAATAGA
AACAAAAGCCGGCTTAAAAATTGCTCAATATAATGATGGTCAAATTTCTGTAAAAATGGGAAAACCAATTATAGATATTA
AGTCTATTCCAACTAATATAGATAAGAAACAAAACTTTCTGCCAGCATTTCTTTTTAAAGAATCTAATTTTGAATATGAA
GGATATGCGGTTGGTATGGGAAATCCACATTTAATATTTTTTGTAGAGGATATCACAAAAATATCTCTTTCTTTATTAGG
TCCTGTTTTTGAAAATCATAAATTATTTCCTGAAAAAACTAATGTACATTTTTCACAAATAATTAATAGAGATACTATTA
AAGTCCTTGTCTGGGAAAGAGGAGCTGGAGCAACACTTGCTTGCGGAACAGGAGCATGTGCAGTACATGTTGCAGCATTT
AATCTGGGATTATGTAATTCAGAAACAATAATAATTCTGCCTGGAGGTAATCTAAAAATAAATTGGTCAAGATTAGAGGA
AGAGATAGTTATGAGCGGTACTGCAAAAAAAGTTTTTTCTGGAAATTATTTTCTAAACTAA

Upstream 100 bases:

>100_bases
CTTTAATAAATTATTATTTTAGTATCCATTGTATAAATAAGGAATAGATTGTTAAAGGTTTAATTTGTTATTAAAATTTA
TGAGATATATTAATTCCTAA

Downstream 100 bases:

>100_bases
ATGTTCAATAATTATATTTATCTTGATAATGCATCAACAACGCCATTATCTAAAAATGTATTAAAAAAAATAAACTCTAC
TTACAATAATTTTTGGAATA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase 2 [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKNIIFEKYHGNGNDFIIIDTRNKNIFNKFISENIFDIKELCDRQFGIGGDGLIFILEPNGNNHARMIIYNSDGSEAQMC
GNGIRCLVQYLHNSKKGSHNSSEYRIETKAGLKIAQYNDGQISVKMGKPIIDIKSIPTNIDKKQNFLPAFLFKESNFEYE
GYAVGMGNPHLIFFVEDITKISLSLLGPVFENHKLFPEKTNVHFSQIINRDTIKVLVWERGAGATLACGTGACAVHVAAF
NLGLCNSETIIILPGGNLKINWSRLEEEIVMSGTAKKVFSGNYFLN

Sequences:

>Translated_286_residues
MKNIIFEKYHGNGNDFIIIDTRNKNIFNKFISENIFDIKELCDRQFGIGGDGLIFILEPNGNNHARMIIYNSDGSEAQMC
GNGIRCLVQYLHNSKKGSHNSSEYRIETKAGLKIAQYNDGQISVKMGKPIIDIKSIPTNIDKKQNFLPAFLFKESNFEYE
GYAVGMGNPHLIFFVEDITKISLSLLGPVFENHKLFPEKTNVHFSQIINRDTIKVLVWERGAGATLACGTGACAVHVAAF
NLGLCNSETIIILPGGNLKINWSRLEEEIVMSGTAKKVFSGNYFLN
>Mature_286_residues
MKNIIFEKYHGNGNDFIIIDTRNKNIFNKFISENIFDIKELCDRQFGIGGDGLIFILEPNGNNHARMIIYNSDGSEAQMC
GNGIRCLVQYLHNSKKGSHNSSEYRIETKAGLKIAQYNDGQISVKMGKPIIDIKSIPTNIDKKQNFLPAFLFKESNFEYE
GYAVGMGNPHLIFFVEDITKISLSLLGPVFENHKLFPEKTNVHFSQIINRDTIKVLVWERGAGATLACGTGACAVHVAAF
NLGLCNSETIIILPGGNLKINWSRLEEEIVMSGTAKKVFSGNYFLN

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=35.7142857142857, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31964; Mature: 31964

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIFEKYHGNGNDFIIIDTRNKNIFNKFISENIFDIKELCDRQFGIGGDGLIFILEPN
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCC
GNNHARMIIYNSDGSEAQMCGNGIRCLVQYLHNSKKGSHNSSEYRIETKAGLKIAQYNDG
CCCEEEEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCC
QISVKMGKPIIDIKSIPTNIDKKQNFLPAFLFKESNFEYEGYAVGMGNPHLIFFVEDITK
EEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCHHH
ISLSLLGPVFENHKLFPEKTNVHFSQIINRDTIKVLVWERGAGATLACGTGACAVHVAAF
HHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEEEEEEE
NLGLCNSETIIILPGGNLKINWSRLEEEIVMSGTAKKVFSGNYFLN
EEECCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCHHEECCCEEEC
>Mature Secondary Structure
MKNIIFEKYHGNGNDFIIIDTRNKNIFNKFISENIFDIKELCDRQFGIGGDGLIFILEPN
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCC
GNNHARMIIYNSDGSEAQMCGNGIRCLVQYLHNSKKGSHNSSEYRIETKAGLKIAQYNDG
CCCEEEEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCC
QISVKMGKPIIDIKSIPTNIDKKQNFLPAFLFKESNFEYEGYAVGMGNPHLIFFVEDITK
EEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCHHH
ISLSLLGPVFENHKLFPEKTNVHFSQIINRDTIKVLVWERGAGATLACGTGACAVHVAAF
HHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEEEEEEE
NLGLCNSETIIILPGGNLKINWSRLEEEIVMSGTAKKVFSGNYFLN
EEECCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCHHEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]