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The map label for this gene is pheT [H]

Identifier: 123966185

GI number: 123966185

Start: 842766

End: 845228

Strand: Direct

Name: pheT [H]

Synonym: P9515_09521

Alternate gene names: 123966185

Gene position: 842766-845228 (Clockwise)

Preceding gene: 123966179

Following gene: 123966186

Centisome position: 49.45

GC content: 29.15

Gene sequence:

>2463_bases
ATGAAGGTTTCTCAAAATTGGTTAAAATCCCTTGTAGATATAAATACTTCACCTGAAGATTTATCTGAAAAATTATCAAT
CGGCGGATTTGAAGTTGAGTCTCTTATTAATTGCTCAGAAAATGTTAAAGGAGTTGTCTTAGGAAAAGTTATATCAGTAG
TTAAACATGATAATTCTGATAAATTATCTATCTGTAATGTTGATATTGGAAGCTCGAATTCTCTACAAATAGTATGTGGA
GCAAAAAATGTAAAAGCAAATATATATGTCTATGTTGCTACTGTTGGAGTCCATTTAAACGCAATCAATTTAACTATCAA
AAAGAGTGAAATAAGAGGAACTTTGAGCGAAGGAATGATATGTTCATTAGAGGAGTTGGGAATTGAAGATAATAGTGAAG
GTATTGCTATTATTGAGGAGGAAAAGGCTTCAAAGCATATATTAGGTACCCCTGCTGCCCTTTTACTTGATTTGAACGAT
TACGTATACGATCTAGCTATAACTGCTAATAGACCTGATGGAATGTCTGTTGTTGGAATTGCTAGAGAAATATCCGCTCT
TCTGGAAACAAATTTAAATCTTCCAAAAATAAAAAATGTATACGAAATTAATGTTCATAAGGATTTTGATCTGTGCACTG
AGGCTATTACAAAAAAATGTCTTTATTCAATTAGTAAAATTGTATCTGTGAGTGGTAAGAAAATTACTCCTCAATGGATT
AAAGATCGTTTAGATAAATCTGGGATTAATTCGATTAATCTTTTGGTGGATATAACTAACTTCATACTTTTAGAGCAAGG
TCAACCATTACATGCATTCGATTTCGATAAATTAACAAATTTAGTTGGTAGAGAGGTTTTACCTGAAGATTTTTCTGTTA
GAAAAGCAAAGGAAAACGAAAGTTTATTGTGTTTAAATGGTGAGATTTATAAATTAAATGAAAACATAACAATTATTGCT
TGTGATGATAAGCCAGTTGCCATAGCGGGTGTGATTGGTGGTTTGCAAACAGCTGTGGATAAAGAAACATCTTCAATTTA
TCTTGAAGGTGCTGTTTTTAATCCCGTTACAATAAGAAAATCAGTAAAAGAAATTGGAGTTAGAACAGAATCTAGTAGTA
GATATGAAAAAGGAATCTCTTTTAAAAATACATTAAATTCTGTAAGCCGCGCTATCAATATAATAGAGGAGTACTTTAAT
ATTACGAAACCTGTAATTCATACTTCAATAGATTTACCGAATACCGAAATATTAATACCACTAAGGAGAAATAGAATTCA
CAAACTACTTGGTCCTTTGGCAATTAGAAATAACGATTATGAGATGAATGGTCAAATACAAAAGAGAAATTTATCAGATA
AAGAAATAACAGATAAATTAAAGCTAATTGGTTGTAAATTAAATAATAAAGAATATGGATGGGATGTTGAAGTTATTCCC
AACAGGTCGCAAGACTTAGTAAGAGAAATAGATTTGATAGAGGAAATTGCACGGTTAATAGGTTATGACATGTTTGATTT
TAATTTGCCAAATCCTATAAAACCAGGAAAACTTTCTTCTTCTCAAATAGCACTAAGGAAGTTAAAAACTGGATTTATAG
ATAATGGCTTTAACGAAGTGTTGTCTTATTCTCTTGTTCCAGAGACTAATAATGATTTAATAAAAATTTCAAATCCATTA
TTAACTGAAACAAGTTGCTTAAGAGATAATATCTGGCAAGAACACATTAAAATTTGTAATCAAAATATAAATTCAGGATA
TGATTATTGTTGGATATTTGAAGTTGGAAATATATTTCATAAAAAACCTGACATATCACAAGAAGAAATCTTAAATGGAG
CAATTTATGGAAATAACAAATTCGATAAATGGTACAGATCAAGTAAAGATGAAAATTTGAACTACTACGAAGCAAGAGGA
AAGTTAAAGGAAGCATTAACGCTTTTAAATATATCAATTGAAGATAAGCCAACTGATTCAATAGATTTTCTTCATCCAGG
TAGGACTGCCAAGTTATTTACTGAAGGTAAAGAAGTTGGTTATTTTGGAGAAATTCATCCAAAATTAGTCTCGAATAAAA
AGGCATTAAAAAAAATGTACCTCTTTAGTTTGAAAATACCCAATATTTTAAATGCATGTACTAGAAAAAATAAATGGATA
CCAGTTTATAAACAATTTCCTACAGTTCCAAAAATGGAACGAGATATTAATTTTATATTCAATAAAAAATACTTAGTAAA
TGAGATAGTCTCACAAATAAAGAAATCTGGCAAAAAATTATTAGAAGAAGTTAATTTAATTGATGTTTATGACGATAATA
GTTTTGGTAATGATTTCATAAGCTATACATTTAGATTGTCATATAGAGATTCTGAAAAAACATTATTAGATTCTGATATC
GTTGATCTACATGAAAACATAGTTGAAATTATTGAAAAAAAATTCTCCACAAAATTAAGATAG

Upstream 100 bases:

>100_bases
TTGATTAATACGCTGATGTATAATTTTCTATTGTACAACTTCAAGAAACTATTTTGCATATATTCACTTTTCTTTTAGTA
TCATTAGACAAAAATAATAA

Downstream 100 bases:

>100_bases
CAGCATTATCGTAAGTCTTATAATAGACTAATAATAGGTCTAATATTAATTCTTATATGAGATAGTTAATATTCATATAA
AAACAACTAATCTTATATAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MKVSQNWLKSLVDINTSPEDLSEKLSIGGFEVESLINCSENVKGVVLGKVISVVKHDNSDKLSICNVDIGSSNSLQIVCG
AKNVKANIYVYVATVGVHLNAINLTIKKSEIRGTLSEGMICSLEELGIEDNSEGIAIIEEEKASKHILGTPAALLLDLND
YVYDLAITANRPDGMSVVGIAREISALLETNLNLPKIKNVYEINVHKDFDLCTEAITKKCLYSISKIVSVSGKKITPQWI
KDRLDKSGINSINLLVDITNFILLEQGQPLHAFDFDKLTNLVGREVLPEDFSVRKAKENESLLCLNGEIYKLNENITIIA
CDDKPVAIAGVIGGLQTAVDKETSSIYLEGAVFNPVTIRKSVKEIGVRTESSSRYEKGISFKNTLNSVSRAINIIEEYFN
ITKPVIHTSIDLPNTEILIPLRRNRIHKLLGPLAIRNNDYEMNGQIQKRNLSDKEITDKLKLIGCKLNNKEYGWDVEVIP
NRSQDLVREIDLIEEIARLIGYDMFDFNLPNPIKPGKLSSSQIALRKLKTGFIDNGFNEVLSYSLVPETNNDLIKISNPL
LTETSCLRDNIWQEHIKICNQNINSGYDYCWIFEVGNIFHKKPDISQEEILNGAIYGNNKFDKWYRSSKDENLNYYEARG
KLKEALTLLNISIEDKPTDSIDFLHPGRTAKLFTEGKEVGYFGEIHPKLVSNKKALKKMYLFSLKIPNILNACTRKNKWI
PVYKQFPTVPKMERDINFIFNKKYLVNEIVSQIKKSGKKLLEEVNLIDVYDDNSFGNDFISYTFRLSYRDSEKTLLDSDI
VDLHENIVEIIEKKFSTKLR

Sequences:

>Translated_820_residues
MKVSQNWLKSLVDINTSPEDLSEKLSIGGFEVESLINCSENVKGVVLGKVISVVKHDNSDKLSICNVDIGSSNSLQIVCG
AKNVKANIYVYVATVGVHLNAINLTIKKSEIRGTLSEGMICSLEELGIEDNSEGIAIIEEEKASKHILGTPAALLLDLND
YVYDLAITANRPDGMSVVGIAREISALLETNLNLPKIKNVYEINVHKDFDLCTEAITKKCLYSISKIVSVSGKKITPQWI
KDRLDKSGINSINLLVDITNFILLEQGQPLHAFDFDKLTNLVGREVLPEDFSVRKAKENESLLCLNGEIYKLNENITIIA
CDDKPVAIAGVIGGLQTAVDKETSSIYLEGAVFNPVTIRKSVKEIGVRTESSSRYEKGISFKNTLNSVSRAINIIEEYFN
ITKPVIHTSIDLPNTEILIPLRRNRIHKLLGPLAIRNNDYEMNGQIQKRNLSDKEITDKLKLIGCKLNNKEYGWDVEVIP
NRSQDLVREIDLIEEIARLIGYDMFDFNLPNPIKPGKLSSSQIALRKLKTGFIDNGFNEVLSYSLVPETNNDLIKISNPL
LTETSCLRDNIWQEHIKICNQNINSGYDYCWIFEVGNIFHKKPDISQEEILNGAIYGNNKFDKWYRSSKDENLNYYEARG
KLKEALTLLNISIEDKPTDSIDFLHPGRTAKLFTEGKEVGYFGEIHPKLVSNKKALKKMYLFSLKIPNILNACTRKNKWI
PVYKQFPTVPKMERDINFIFNKKYLVNEIVSQIKKSGKKLLEEVNLIDVYDDNSFGNDFISYTFRLSYRDSEKTLLDSDI
VDLHENIVEIIEKKFSTKLR
>Mature_820_residues
MKVSQNWLKSLVDINTSPEDLSEKLSIGGFEVESLINCSENVKGVVLGKVISVVKHDNSDKLSICNVDIGSSNSLQIVCG
AKNVKANIYVYVATVGVHLNAINLTIKKSEIRGTLSEGMICSLEELGIEDNSEGIAIIEEEKASKHILGTPAALLLDLND
YVYDLAITANRPDGMSVVGIAREISALLETNLNLPKIKNVYEINVHKDFDLCTEAITKKCLYSISKIVSVSGKKITPQWI
KDRLDKSGINSINLLVDITNFILLEQGQPLHAFDFDKLTNLVGREVLPEDFSVRKAKENESLLCLNGEIYKLNENITIIA
CDDKPVAIAGVIGGLQTAVDKETSSIYLEGAVFNPVTIRKSVKEIGVRTESSSRYEKGISFKNTLNSVSRAINIIEEYFN
ITKPVIHTSIDLPNTEILIPLRRNRIHKLLGPLAIRNNDYEMNGQIQKRNLSDKEITDKLKLIGCKLNNKEYGWDVEVIP
NRSQDLVREIDLIEEIARLIGYDMFDFNLPNPIKPGKLSSSQIALRKLKTGFIDNGFNEVLSYSLVPETNNDLIKISNPL
LTETSCLRDNIWQEHIKICNQNINSGYDYCWIFEVGNIFHKKPDISQEEILNGAIYGNNKFDKWYRSSKDENLNYYEARG
KLKEALTLLNISIEDKPTDSIDFLHPGRTAKLFTEGKEVGYFGEIHPKLVSNKKALKKMYLFSLKIPNILNACTRKNKWI
PVYKQFPTVPKMERDINFIFNKKYLVNEIVSQIKKSGKKLLEEVNLIDVYDDNSFGNDFISYTFRLSYRDSEKTLLDSDI
VDLHENIVEIIEKKFSTKLR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=850, Percent_Identity=29.5294117647059, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 92650; Mature: 92650

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSQNWLKSLVDINTSPEDLSEKLSIGGFEVESLINCSENVKGVVLGKVISVVKHDNSD
CCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHHHCCCCCCCEEHHHHHHHHHHCCCCC
KLSICNVDIGSSNSLQIVCGAKNVKANIYVYVATVGVHLNAINLTIKKSEIRGTLSEGMI
CEEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEEEEEEEEEEEEEEHHHHCCHHHCCCE
CSLEELGIEDNSEGIAIIEEEKASKHILGTPAALLLDLNDYVYDLAITANRPDGMSVVGI
EEHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCEEHHH
AREISALLETNLNLPKIKNVYEINVHKDFDLCTEAITKKCLYSISKIVSVSGKKITPQWI
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDRLDKSGINSINLLVDITNFILLEQGQPLHAFDFDKLTNLVGREVLPEDFSVRKAKENE
HHHHHHCCCCCEEEEEEEEHEEEECCCCCCCEECHHHHHHHHHHHCCCCCCCHHCCCCCC
SLLCLNGEIYKLNENITIIACDDKPVAIAGVIGGLQTAVDKETSSIYLEGAVFNPVTIRK
CEEEECCEEEEECCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEECEEECCHHHHH
SVKEIGVRTESSSRYEKGISFKNTLNSVSRAINIIEEYFNITKPVIHTSIDLPNTEILIP
HHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEE
LRRNRIHKLLGPLAIRNNDYEMNGQIQKRNLSDKEITDKLKLIGCKLNNKEYGWDVEVIP
CHHHHHHHHHCCCEECCCCEEECCEEEECCCCHHHHHHHEEEEEEEECCCCCCEEEEECC
NRSQDLVREIDLIEEIARLIGYDMFDFNLPNPIKPGKLSSSQIALRKLKTGFIDNGFNEV
CCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LSYSLVPETNNDLIKISNPLLTETSCLRDNIWQEHIKICNQNINSGYDYCWIFEVGNIFH
HEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHC
KKPDISQEEILNGAIYGNNKFDKWYRSSKDENLNYYEARGKLKEALTLLNISIEDKPTDS
CCCCCCHHHHHCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCC
IDFLHPGRTAKLFTEGKEVGYFGEIHPKLVSNKKALKKMYLFSLKIPNILNACTRKNKWI
CCCCCCCCCEEEEECCCCCCEECCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCE
PVYKQFPTVPKMERDINFIFNKKYLVNEIVSQIKKSGKKLLEEVNLIDVYDDNSFGNDFI
EHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEE
SYTFRLSYRDSEKTLLDSDIVDLHENIVEIIEKKFSTKLR
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVSQNWLKSLVDINTSPEDLSEKLSIGGFEVESLINCSENVKGVVLGKVISVVKHDNSD
CCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHHHCCCCCCCEEHHHHHHHHHHCCCCC
KLSICNVDIGSSNSLQIVCGAKNVKANIYVYVATVGVHLNAINLTIKKSEIRGTLSEGMI
CEEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEEEEEEEEEEEEEEHHHHCCHHHCCCE
CSLEELGIEDNSEGIAIIEEEKASKHILGTPAALLLDLNDYVYDLAITANRPDGMSVVGI
EEHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCEEHHH
AREISALLETNLNLPKIKNVYEINVHKDFDLCTEAITKKCLYSISKIVSVSGKKITPQWI
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDRLDKSGINSINLLVDITNFILLEQGQPLHAFDFDKLTNLVGREVLPEDFSVRKAKENE
HHHHHHCCCCCEEEEEEEEHEEEECCCCCCCEECHHHHHHHHHHHCCCCCCCHHCCCCCC
SLLCLNGEIYKLNENITIIACDDKPVAIAGVIGGLQTAVDKETSSIYLEGAVFNPVTIRK
CEEEECCEEEEECCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEECEEECCHHHHH
SVKEIGVRTESSSRYEKGISFKNTLNSVSRAINIIEEYFNITKPVIHTSIDLPNTEILIP
HHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEE
LRRNRIHKLLGPLAIRNNDYEMNGQIQKRNLSDKEITDKLKLIGCKLNNKEYGWDVEVIP
CHHHHHHHHHCCCEECCCCEEECCEEEECCCCHHHHHHHEEEEEEEECCCCCCEEEEECC
NRSQDLVREIDLIEEIARLIGYDMFDFNLPNPIKPGKLSSSQIALRKLKTGFIDNGFNEV
CCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LSYSLVPETNNDLIKISNPLLTETSCLRDNIWQEHIKICNQNINSGYDYCWIFEVGNIFH
HEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHC
KKPDISQEEILNGAIYGNNKFDKWYRSSKDENLNYYEARGKLKEALTLLNISIEDKPTDS
CCCCCCHHHHHCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCC
IDFLHPGRTAKLFTEGKEVGYFGEIHPKLVSNKKALKKMYLFSLKIPNILNACTRKNKWI
CCCCCCCCCEEEEECCCCCCEECCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCE
PVYKQFPTVPKMERDINFIFNKKYLVNEIVSQIKKSGKKLLEEVNLIDVYDDNSFGNDFI
EHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEE
SYTFRLSYRDSEKTLLDSDIVDLHENIVEIIEKKFSTKLR
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]