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The map label for this gene is metG [H]

Identifier: 123966181

GI number: 123966181

Start: 839411

End: 840946

Strand: Reverse

Name: metG [H]

Synonym: P9515_09481

Alternate gene names: 123966181

Gene position: 840946-839411 (Counterclockwise)

Preceding gene: 123966182

Following gene: 123966180

Centisome position: 49.35

GC content: 29.1

Gene sequence:

>1536_bases
ATGAGCTTTGTAATTACTACCCCATTATATTATGTAAATGATAAGCCTCACTTAGGGAGCATTTATACTACTTTAATTTG
TGATTCCATAGCAAGGTTCAAGAGACTATCAGGCGATAATGTAATTTTCATTACTGGAGTTGATGAACATGGTTTAAAGA
TTCAAAGAACTGCTAGTAATAAAGGTGTAGATCCTCAATCTCATTGTGATGAGATTTCTGATATTTTTAAACAAAATTGG
AAATCTTGGGACATAACAAATAATAAATTTGTAAGAACCAGTTCTCAAAATCATGAATCTATAGTTAATGAATTTTACAA
CAGAGTAAAAGCTTCTGGAGATATCTATATGGGAGTCCAAAAAGGATGGTATTGCGTCGGTTGTGAAGAATTTAAAGAAA
ATCCAGAAAATCTATCATCATATAAATGTCCAATTCATCAAAAAAACTTAGAATGGAAAAATGAAGAAAATCTTTTTTTT
AGATTATCTAAATATCAATCACAAATCGAAGAACTTATTAATGAGCCTTCATTTATTTATCCCAAAGAAAGAAGAAATGA
AATTATCAATTTCGTAAGCAAAGGTTTAAAAGATTTCTCAGTATCTAGAACAAATGTATCTTGGGGAATATCAGTACCCG
ATACAAAGGATCATACTTTTTACGTATGGTTTGATGCTTTACTTGGCTATGTAAGTGCTATATGCCCAAATATGAGTGAT
CCATCATTGGAATCATCTATTAATAATGGATGGCCTGCTGACATACATTTTATAGGTAAGGATATCCTAAGGTTTCATGC
TGTTTATTGGCCAGCGATGCTTCTTTCAGCTGGTATGGATGTACCTAAAAGAGTATTTGGTCATGGCTTCTTAACAAGAG
AAGGTCAAAAAATGGGGAAAAGCTTAGGAAATGTATTAGATCCAAATCTTTTATTATCTAAATATGGTAAGGAAGCAGTT
AGATGGTATCTTTTAAAGGATATTTCATTGGGTAGTGATGGTGATTTTCAAAATAAAAGATTTGTAGACATTATTAATAA
TGATTTGGCTAACACAATTGGAAATTTATTAAATAGAACATCTTCAATGTCTAGAAAGTGGTTTGACAATAAAGTTCCAG
ATACTTATGAGTTAATCATTGATAATACATTGCAATTATGCTGCAATGAAGCAGTAGAAAAATTTATAAATCATTTTAAT
TTATATGAGTTAGATTTAGCAGCTAATGTTATACTTAATCTTGCAATTAATACAAATTTATATCTTAACGAAAAACAGCC
TTGGAAATTAATTAAAGATGAAAATAATATTTCTATTGTATGTAATATAATTTATGATGTATTAGAAAGTACAAGAATTA
TTGGCTTATTATTATTACCAATATTACCGGATTTATCCTCCAAAATTAACATGCAATTGGGATCTCTATATAATAAAAAT
GAGTCTTGGAATGATCAATTGAATTGGGGTTTATTGAAATCTAAATCAAATTTACCAGCTCCATATCCAATTATTGAAAA
ACTAGAATATGAATAG

Upstream 100 bases:

>100_bases
AAAATGATAATAATCTTTTAGAATTTATTCACTAGCTATAAATAAACTCTATATCATAATATTTAGATATTATATTTGTT
ACTATTAGAAAAAAGATATT

Downstream 100 bases:

>100_bases
ATTTTATAAACTTTTTTTAATAACTACAATAATTATTGGAGGATGTAAAACTCGAGATATAGAAGATAAAATAACTAACC
AAAGTATTAATAGTTTAAAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSFVITTPLYYVNDKPHLGSIYTTLICDSIARFKRLSGDNVIFITGVDEHGLKIQRTASNKGVDPQSHCDEISDIFKQNW
KSWDITNNKFVRTSSQNHESIVNEFYNRVKASGDIYMGVQKGWYCVGCEEFKENPENLSSYKCPIHQKNLEWKNEENLFF
RLSKYQSQIEELINEPSFIYPKERRNEIINFVSKGLKDFSVSRTNVSWGISVPDTKDHTFYVWFDALLGYVSAICPNMSD
PSLESSINNGWPADIHFIGKDILRFHAVYWPAMLLSAGMDVPKRVFGHGFLTREGQKMGKSLGNVLDPNLLLSKYGKEAV
RWYLLKDISLGSDGDFQNKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPDTYELIIDNTLQLCCNEAVEKFINHFN
LYELDLAANVILNLAINTNLYLNEKQPWKLIKDENNISIVCNIIYDVLESTRIIGLLLLPILPDLSSKINMQLGSLYNKN
ESWNDQLNWGLLKSKSNLPAPYPIIEKLEYE

Sequences:

>Translated_511_residues
MSFVITTPLYYVNDKPHLGSIYTTLICDSIARFKRLSGDNVIFITGVDEHGLKIQRTASNKGVDPQSHCDEISDIFKQNW
KSWDITNNKFVRTSSQNHESIVNEFYNRVKASGDIYMGVQKGWYCVGCEEFKENPENLSSYKCPIHQKNLEWKNEENLFF
RLSKYQSQIEELINEPSFIYPKERRNEIINFVSKGLKDFSVSRTNVSWGISVPDTKDHTFYVWFDALLGYVSAICPNMSD
PSLESSINNGWPADIHFIGKDILRFHAVYWPAMLLSAGMDVPKRVFGHGFLTREGQKMGKSLGNVLDPNLLLSKYGKEAV
RWYLLKDISLGSDGDFQNKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPDTYELIIDNTLQLCCNEAVEKFINHFN
LYELDLAANVILNLAINTNLYLNEKQPWKLIKDENNISIVCNIIYDVLESTRIIGLLLLPILPDLSSKINMQLGSLYNKN
ESWNDQLNWGLLKSKSNLPAPYPIIEKLEYE
>Mature_510_residues
SFVITTPLYYVNDKPHLGSIYTTLICDSIARFKRLSGDNVIFITGVDEHGLKIQRTASNKGVDPQSHCDEISDIFKQNWK
SWDITNNKFVRTSSQNHESIVNEFYNRVKASGDIYMGVQKGWYCVGCEEFKENPENLSSYKCPIHQKNLEWKNEENLFFR
LSKYQSQIEELINEPSFIYPKERRNEIINFVSKGLKDFSVSRTNVSWGISVPDTKDHTFYVWFDALLGYVSAICPNMSDP
SLESSINNGWPADIHFIGKDILRFHAVYWPAMLLSAGMDVPKRVFGHGFLTREGQKMGKSLGNVLDPNLLLSKYGKEAVR
WYLLKDISLGSDGDFQNKRFVDIINNDLANTIGNLLNRTSSMSRKWFDNKVPDTYELIIDNTLQLCCNEAVEKFINHFNL
YELDLAANVILNLAINTNLYLNEKQPWKLIKDENNISIVCNIIYDVLESTRIIGLLLLPILPDLSSKINMQLGSLYNKNE
SWNDQLNWGLLKSKSNLPAPYPIIEKLEYE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=501, Percent_Identity=33.1337325349301, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI14043022, Length=511, Percent_Identity=24.853228962818, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1788432, Length=532, Percent_Identity=25.5639097744361, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI212645096, Length=504, Percent_Identity=31.1507936507937, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=30.5555555555556, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17540794, Length=525, Percent_Identity=26.2857142857143, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321610, Length=509, Percent_Identity=32.6129666011788, Blast_Score=256, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=518, Percent_Identity=25.8687258687259, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21358483, Length=502, Percent_Identity=31.8725099601594, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24655311, Length=546, Percent_Identity=25.0915750915751, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58758; Mature: 58626

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVITTPLYYVNDKPHLGSIYTTLICDSIARFKRLSGDNVIFITGVDEHGLKIQRTASN
CCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCC
KGVDPQSHCDEISDIFKQNWKSWDITNNKFVRTSSQNHESIVNEFYNRVKASGDIYMGVQ
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKENPENLSSYKCPIHQKNLEWKNEENLFFRLSKYQSQIEELINEPSFIY
CCEEEEECHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEC
PKERRNEIINFVSKGLKDFSVSRTNVSWGISVPDTKDHTFYVWFDALLGYVSAICPNMSD
CHHHHHHHHHHHHHCHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCC
PSLESSINNGWPADIHFIGKDILRFHAVYWPAMLLSAGMDVPKRVFGHGFLTREGQKMGK
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHH
SLGNVLDPNLLLSKYGKEAVRWYLLKDISLGSDGDFQNKRFVDIINNDLANTIGNLLNRT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SSMSRKWFDNKVPDTYELIIDNTLQLCCNEAVEKFINHFNLYELDLAANVILNLAINTNL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHEEEEECCEE
YLNEKQPWKLIKDENNISIVCNIIYDVLESTRIIGLLLLPILPDLSSKINMQLGSLYNKN
EECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
ESWNDQLNWGLLKSKSNLPAPYPIIEKLEYE
CCCCCCCCCCEEECCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SFVITTPLYYVNDKPHLGSIYTTLICDSIARFKRLSGDNVIFITGVDEHGLKIQRTASN
CEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCC
KGVDPQSHCDEISDIFKQNWKSWDITNNKFVRTSSQNHESIVNEFYNRVKASGDIYMGVQ
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
KGWYCVGCEEFKENPENLSSYKCPIHQKNLEWKNEENLFFRLSKYQSQIEELINEPSFIY
CCEEEEECHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEC
PKERRNEIINFVSKGLKDFSVSRTNVSWGISVPDTKDHTFYVWFDALLGYVSAICPNMSD
CHHHHHHHHHHHHHCHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCC
PSLESSINNGWPADIHFIGKDILRFHAVYWPAMLLSAGMDVPKRVFGHGFLTREGQKMGK
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHH
SLGNVLDPNLLLSKYGKEAVRWYLLKDISLGSDGDFQNKRFVDIINNDLANTIGNLLNRT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SSMSRKWFDNKVPDTYELIIDNTLQLCCNEAVEKFINHFNLYELDLAANVILNLAINTNL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHEEEEECCEE
YLNEKQPWKLIKDENNISIVCNIIYDVLESTRIIGLLLLPILPDLSSKINMQLGSLYNKN
EECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
ESWNDQLNWGLLKSKSNLPAPYPIIEKLEYE
CCCCCCCCCCEEECCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]