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The map label for this gene is vacB [H]

Identifier: 123966156

GI number: 123966156

Start: 826238

End: 828460

Strand: Reverse

Name: vacB [H]

Synonym: P9515_09231

Alternate gene names: 123966156

Gene position: 828460-826238 (Counterclockwise)

Preceding gene: 123966157

Following gene: 123966155

Centisome position: 48.61

GC content: 25.78

Gene sequence:

>2223_bases
ATGTTTTCAACCTCCTCAATAATTGATAAATTAAAACAAGAGGAAGGATTGGAATATAAAAAATTATGCAGATTATTAAA
ATTATCAAAAAATGCGGATAAAGATAAGTTAGATATTGCATTAAGAGCATTAGAAGAACTTGAAATAATAGATAAAAATG
AAAATAATGAATATTTCAATGTTAAAGAAAGTAATCATTTAGTTGCGAGGATTAGATGTAGCAGCAAGGGATATTGCTTT
GCAGTTAGAGAAAACAATAAGGAAGATATATACATCAAAGAAAATTTACTTAATTGTGCCTGGAATGGAGATAAAGTTCT
TGTAAGAATAATAAAAGAAGGAGTTAGAAGAAGATCACCTGAAGGAATAGTAGATTGTATACTTGAAAGAACAAACCAAA
TACTTTTAGCAAAAGTTGAAGTAATCAACGAGGAGATATATGGAGTACCTATTGATGACAGAATTCTATCTAAAATAAAA
CTCCCAAAAGAAGATGAAAAATATAAATATATTCCTGATAATAAAAATATTGTAAAAATAGAGATTGACCTATTCCCAAT
TGGACAACAAGACGGAGTTGGTCATGTCATAAAGGAATTAAAACTAAATAATAACGAAAAACTAGATGCAGATTTCGTAT
TATCTAAAAGTAATCTTAATAGAATTATCAAACTTAAAGATCCAAAAATTGCTGGAAAAAATGAACTTCCAAGATTAGAT
TTAACGAATAAAAATTCATATATGTTCAAAAGTTGGAATGGTGAAAATTCACCAACACTTCCAATATTTCAAATAGACAA
AAATAAAGATGATTCATATAAACTCTGGGTTCACACAAATACAATTGCAGAAAAAATAGATCTAAGTAGTAAGAAGGGCT
TAGAAGTATTTTTTAATAGTTTTGAATCATTCCCTTTGCTAGAAAAATGGCAACCTTATATTAGTGAGGAAATATGCAAT
TCTTCAAAATTTAATGTAGGAGAAATTAATGAAGCTATAAGTTTATGTATAGATATTAATACTGAGAATGAAATAACAAA
CTGGTCATTCCATTTGACAAAAGTAAAATGTACACTTTTAGTGGAGAATAAACATTTAGATGCCCTATTAACGAGGAAAA
GTAGTACTAGAATTACGTCGAGAATCCTCAAACCTATTAAGGATTACATAGAAGATTTAGATAAAATTATAGAAATATCT
AAGTTTTTTAGAACAAAACAAATATTGCATGGAAAAATTGAGATACCAAAACCAAATAGTAATATAGATTCTTTAAGGGA
ATTATTAGTCCACAATCCTGTTGACTATTCAAAGGAATATTTTGAACCATTAAATAATAATGATATTCAATCATATTTAT
CACCTTTACTATACGAGGCTAATTCATTATGGTTTAAACATTCAAATAAATACGGCTTAATTAATGCTTCATATATTTCC
CAAGAGTTAGATTACATAAATATTAGTGAAATTATTAAAAATTCAGAATTAATAAATAGTGATATCGAGCTAAATGAAAA
TGGCACCTTAACATTTAAGCAATTAGTTGAATTATGTAGCGATGATGATAATAAAAAAAGAATTTTGCATAAGTTATTAA
TAAATGTTTTAAGAGATAATGAAGTGAAACTGATAACAAAAGATTTTAATAAAATAGATTCTAAAAAAATTATTTCACCA
TGGACTTTACCATCATACGATTTTATAAATTTAATTAATCAATACAATATCTATAACATGATAATAAATGGTAAAAGAAC
CAAAAATACTAAAGGAAATATTATTAATATTATGGAAAAGGACACTTGGAAAGATGTTAGTTGGAGTATTTATAATTCAA
CAACTTTAAAAAGTATGGATTTATTATTTAATCAACATTTAATAGACAAAATTAACGAATATAAAAATAAAGTAAGGCAA
TTCAAGTTAAATATGATTAATATAAAACAAGTAAGAAAAGCAGAAAAACTTATAGGAAATACATATAGCGGTTTAATAAT
GACAGTTCAAAGTTATGGCTTTTTCGTTGAGATAAGAGAGTTATTTGTGGAAGGTTTAGTACATGTAAGTACCCTAAGTA
ATGATTGGTACGAATATAGATCTCGACAAAACGTATTAATTGGCAGAAAATCAAAGAAATCTTATAAGATTGGAGATATA
ATTGATGTCAAAATTATTAAGGTTGATATTCTTAAATATCAAATAGATCTTGAACTTGTTTAG

Upstream 100 bases:

>100_bases
AAGCTTATAATAAAGAAAGGAATATTAATTAGTTTTTGATTAAAAAGAAAGATATAATGTAAACGTATCTCTACACATCA
GTTGAATATCTAGGGTCATT

Downstream 100 bases:

>100_bases
AGATAACTAATCTATATACTCTAAACAATTTAATTAATTTTTATTAATAACTTACAATAAAATGAATTATAAAAAAAAAG
GTATATTATTAAGTATTTTC

Product: putative acetazolamide conferring resistance protein Zam

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MFSTSSIIDKLKQEEGLEYKKLCRLLKLSKNADKDKLDIALRALEELEIIDKNENNEYFNVKESNHLVARIRCSSKGYCF
AVRENNKEDIYIKENLLNCAWNGDKVLVRIIKEGVRRRSPEGIVDCILERTNQILLAKVEVINEEIYGVPIDDRILSKIK
LPKEDEKYKYIPDNKNIVKIEIDLFPIGQQDGVGHVIKELKLNNNEKLDADFVLSKSNLNRIIKLKDPKIAGKNELPRLD
LTNKNSYMFKSWNGENSPTLPIFQIDKNKDDSYKLWVHTNTIAEKIDLSSKKGLEVFFNSFESFPLLEKWQPYISEEICN
SSKFNVGEINEAISLCIDINTENEITNWSFHLTKVKCTLLVENKHLDALLTRKSSTRITSRILKPIKDYIEDLDKIIEIS
KFFRTKQILHGKIEIPKPNSNIDSLRELLVHNPVDYSKEYFEPLNNNDIQSYLSPLLYEANSLWFKHSNKYGLINASYIS
QELDYINISEIIKNSELINSDIELNENGTLTFKQLVELCSDDDNKKRILHKLLINVLRDNEVKLITKDFNKIDSKKIISP
WTLPSYDFINLINQYNIYNMIINGKRTKNTKGNIINIMEKDTWKDVSWSIYNSTTLKSMDLLFNQHLIDKINEYKNKVRQ
FKLNMINIKQVRKAEKLIGNTYSGLIMTVQSYGFFVEIRELFVEGLVHVSTLSNDWYEYRSRQNVLIGRKSKKSYKIGDI
IDVKIIKVDILKYQIDLELV

Sequences:

>Translated_740_residues
MFSTSSIIDKLKQEEGLEYKKLCRLLKLSKNADKDKLDIALRALEELEIIDKNENNEYFNVKESNHLVARIRCSSKGYCF
AVRENNKEDIYIKENLLNCAWNGDKVLVRIIKEGVRRRSPEGIVDCILERTNQILLAKVEVINEEIYGVPIDDRILSKIK
LPKEDEKYKYIPDNKNIVKIEIDLFPIGQQDGVGHVIKELKLNNNEKLDADFVLSKSNLNRIIKLKDPKIAGKNELPRLD
LTNKNSYMFKSWNGENSPTLPIFQIDKNKDDSYKLWVHTNTIAEKIDLSSKKGLEVFFNSFESFPLLEKWQPYISEEICN
SSKFNVGEINEAISLCIDINTENEITNWSFHLTKVKCTLLVENKHLDALLTRKSSTRITSRILKPIKDYIEDLDKIIEIS
KFFRTKQILHGKIEIPKPNSNIDSLRELLVHNPVDYSKEYFEPLNNNDIQSYLSPLLYEANSLWFKHSNKYGLINASYIS
QELDYINISEIIKNSELINSDIELNENGTLTFKQLVELCSDDDNKKRILHKLLINVLRDNEVKLITKDFNKIDSKKIISP
WTLPSYDFINLINQYNIYNMIINGKRTKNTKGNIINIMEKDTWKDVSWSIYNSTTLKSMDLLFNQHLIDKINEYKNKVRQ
FKLNMINIKQVRKAEKLIGNTYSGLIMTVQSYGFFVEIRELFVEGLVHVSTLSNDWYEYRSRQNVLIGRKSKKSYKIGDI
IDVKIIKVDILKYQIDLELV
>Mature_740_residues
MFSTSSIIDKLKQEEGLEYKKLCRLLKLSKNADKDKLDIALRALEELEIIDKNENNEYFNVKESNHLVARIRCSSKGYCF
AVRENNKEDIYIKENLLNCAWNGDKVLVRIIKEGVRRRSPEGIVDCILERTNQILLAKVEVINEEIYGVPIDDRILSKIK
LPKEDEKYKYIPDNKNIVKIEIDLFPIGQQDGVGHVIKELKLNNNEKLDADFVLSKSNLNRIIKLKDPKIAGKNELPRLD
LTNKNSYMFKSWNGENSPTLPIFQIDKNKDDSYKLWVHTNTIAEKIDLSSKKGLEVFFNSFESFPLLEKWQPYISEEICN
SSKFNVGEINEAISLCIDINTENEITNWSFHLTKVKCTLLVENKHLDALLTRKSSTRITSRILKPIKDYIEDLDKIIEIS
KFFRTKQILHGKIEIPKPNSNIDSLRELLVHNPVDYSKEYFEPLNNNDIQSYLSPLLYEANSLWFKHSNKYGLINASYIS
QELDYINISEIIKNSELINSDIELNENGTLTFKQLVELCSDDDNKKRILHKLLINVLRDNEVKLITKDFNKIDSKKIISP
WTLPSYDFINLINQYNIYNMIINGKRTKNTKGNIINIMEKDTWKDVSWSIYNSTTLKSMDLLFNQHLIDKINEYKNKVRQ
FKLNMINIKQVRKAEKLIGNTYSGLIMTVQSYGFFVEIRELFVEGLVHVSTLSNDWYEYRSRQNVLIGRKSKKSYKIGDI
IDVKIIKVDILKYQIDLELV

Specific function: Not known; control resistance to the carbonic anhydrase inhibitor acetazolamide [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082383, Length=83, Percent_Identity=38.5542168674699, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 86330; Mature: 86330

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSTSSIIDKLKQEEGLEYKKLCRLLKLSKNADKDKLDIALRALEELEIIDKNENNEYFN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
VKESNHLVARIRCSSKGYCFAVRENNKEDIYIKENLLNCAWNGDKVLVRIIKEGVRRRSP
EECCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCCC
EGIVDCILERTNQILLAKVEVINEEIYGVPIDDRILSKIKLPKEDEKYKYIPDNKNIVKI
CHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEECCCCCCEEEE
EIDLFPIGQQDGVGHVIKELKLNNNEKLDADFVLSKSNLNRIIKLKDPKIAGKNELPRLD
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCEE
LTNKNSYMFKSWNGENSPTLPIFQIDKNKDDSYKLWVHTNTIAEKIDLSSKKGLEVFFNS
ECCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHH
FESFPLLEKWQPYISEEICNSSKFNVGEINEAISLCIDINTENEITNWSFHLTKVKCTLL
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCEEEEEEECCCCCCCCCEEEEEEEEEEEEE
VENKHLDALLTRKSSTRITSRILKPIKDYIEDLDKIIEISKFFRTKQILHGKIEIPKPNS
EECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
NIDSLRELLVHNPVDYSKEYFEPLNNNDIQSYLSPLLYEANSLWFKHSNKYGLINASYIS
CHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEHHHHH
QELDYINISEIIKNSELINSDIELNENGTLTFKQLVELCSDDDNKKRILHKLLINVLRDN
HCCCEECHHHHHCCHHHCCCCCEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
EVKLITKDFNKIDSKKIISPWTLPSYDFINLINQYNIYNMIINGKRTKNTKGNIINIMEK
CEEEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEC
DTWKDVSWSIYNSTTLKSMDLLFNQHLIDKINEYKNKVRQFKLNMINIKQVRKAEKLIGN
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECHHHHHHHHHHHCC
TYSGLIMTVQSYGFFVEIRELFVEGLVHVSTLSNDWYEYRSRQNVLIGRKSKKSYKIGDI
CCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCCCE
IDVKIIKVDILKYQIDLELV
EEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MFSTSSIIDKLKQEEGLEYKKLCRLLKLSKNADKDKLDIALRALEELEIIDKNENNEYFN
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
VKESNHLVARIRCSSKGYCFAVRENNKEDIYIKENLLNCAWNGDKVLVRIIKEGVRRRSP
EECCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCCC
EGIVDCILERTNQILLAKVEVINEEIYGVPIDDRILSKIKLPKEDEKYKYIPDNKNIVKI
CHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEECCCCCCEEEE
EIDLFPIGQQDGVGHVIKELKLNNNEKLDADFVLSKSNLNRIIKLKDPKIAGKNELPRLD
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCEE
LTNKNSYMFKSWNGENSPTLPIFQIDKNKDDSYKLWVHTNTIAEKIDLSSKKGLEVFFNS
ECCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHH
FESFPLLEKWQPYISEEICNSSKFNVGEINEAISLCIDINTENEITNWSFHLTKVKCTLL
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCEEEEEEECCCCCCCCCEEEEEEEEEEEEE
VENKHLDALLTRKSSTRITSRILKPIKDYIEDLDKIIEISKFFRTKQILHGKIEIPKPNS
EECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
NIDSLRELLVHNPVDYSKEYFEPLNNNDIQSYLSPLLYEANSLWFKHSNKYGLINASYIS
CHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEHHHHH
QELDYINISEIIKNSELINSDIELNENGTLTFKQLVELCSDDDNKKRILHKLLINVLRDN
HCCCEECHHHHHCCHHHCCCCCEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
EVKLITKDFNKIDSKKIISPWTLPSYDFINLINQYNIYNMIINGKRTKNTKGNIINIMEK
CEEEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEC
DTWKDVSWSIYNSTTLKSMDLLFNQHLIDKINEYKNKVRQFKLNMINIKQVRKAEKLIGN
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECHHHHHHHHHHHCC
TYSGLIMTVQSYGFFVEIRELFVEGLVHVSTLSNDWYEYRSRQNVLIGRKSKKSYKIGDI
CCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCCCCCCE
IDVKIIKVDILKYQIDLELV
EEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727754; 8905231 [H]