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The map label for this gene is pmbA [H]

Identifier: 123966151

GI number: 123966151

Start: 820607

End: 821959

Strand: Reverse

Name: pmbA [H]

Synonym: P9515_09181

Alternate gene names: 123966151

Gene position: 821959-820607 (Counterclockwise)

Preceding gene: 123966152

Following gene: 123966150

Centisome position: 48.23

GC content: 32.3

Gene sequence:

>1353_bases
ATGAATGCAACAGAAATAACAAATCAAATAAAACACACAGCAAATATATTAAATATCGATAAATGGGATTACGGAGCTAG
TTTTTCTAATGATTATTCTGTTCAAGTTGATAGAGGTGCAGCAAAACAATTAAAAGCTTCTCAAAAACAGGTTATAACGT
TGAGAGTTTGGAATAAAGAAAATTTAGTTGGTATAACTACGACAAGTGATTTAAGTGAAACTGGAATCAAAAAAGCCTTA
AAACAAGCAAATATTGCCTCAGAATTTGGAAATAAAAATGAATTTACCGATTTTTCTCCATTAGCCAAAGATCCTATACA
AGTTAAAGAAATAGATAAAAAGAATCCATTAGGAATTAAAAAGCTACTTAAAATTCTTAGGGAAGCCGAATCGAAACTTA
TTGACAGTCACGAAGCTATTAAATCTATTCCTTACAATGGTTTGTCAGAAAGTTTTTACGAAAGAATTTATGCCAATAGT
GATGGCGCATTTAGAAACTACAGTAAAAGTCAAGCTGCTTTATATTTATATGCCAGAGCAGAAGAAAAAGATAAAAAACC
CAGGAGTTCAGGTTCAGTTAAACTTGGTTATGGTGTCGAAGACATTGATATTGAATCATGCATTAAAGAAGCTTCAAAGA
AAACAATTGCACATTTAGACTATTCATCAATAAAAACTGGTAAGTATTTAGTTTGTCTATCACCTGAAGCCTTTCTGACA
ATAATAAATGCCTTTAGTTCACTCTTCAATGCAAGAAGCATTATTGATGGTGTAAGTCTTTCAGATAAAAACTCCATAGG
CGACATGATTTCAATCTCAGCTTTGAATATCTATGATGATGGACTTAATGATAAAAATATATCTTCAGCACCATTCGATG
GTGAAGGCACTCCAACAAAAAAACTGAGCATAATTAAAAAAGGTAAATTAGAAAACTTTCTACATTCTGAATCGACAGCA
AAAATTTTTAATACCATACCTACTGGTCATGCAGGTCTTGGTTCAAAAGTATCGGTTTCTCCAGATTGGCTAGTAATTGA
AAGATCAAGCGATTGTTCGGAACTAAATATGTCATTGGAGCATACAAATTATGAAGGAGAGTTTGTATATATTGAAGAAT
TGAATGCTATTCATGCAGGAGTAAGAGCCAGTCAAGGATCCTTTTCTCTTCCTTTCGAGGGATGGATTTATAGTAATGGG
AAAAAATCCTCAATTGAGTCTGCGACTGTAGCAGGGGATATAAAATATCTTCTTAGAAATATAGTTAATATTGAAAATGA
AGAAGAATTAACTACAAGTGGAATTTCCCCACATATTTGGATTAGTGAATTATCAATAACTGGAGACGAGTGA

Upstream 100 bases:

>100_bases
TAGCACCAGGTTTTTGTGGCTCAGTAAGCGGAAGCGTAAATGTAACAGTCGGTCAGCCACATATAAAAGTTGATTCAATA
ACTGTAGGAGGTAGATAAAA

Downstream 100 bases:

>100_bases
GAATTATATTTTGGGGAACTCCTGAATATTCAGTAAAAAGTCTTGAAGTATTAAAAAAATCAGATCATGAAATTTTAGCT
GTCATAACGCAGCCAGATAA

Product: putative modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNATEITNQIKHTANILNIDKWDYGASFSNDYSVQVDRGAAKQLKASQKQVITLRVWNKENLVGITTTSDLSETGIKKAL
KQANIASEFGNKNEFTDFSPLAKDPIQVKEIDKKNPLGIKKLLKILREAESKLIDSHEAIKSIPYNGLSESFYERIYANS
DGAFRNYSKSQAALYLYARAEEKDKKPRSSGSVKLGYGVEDIDIESCIKEASKKTIAHLDYSSIKTGKYLVCLSPEAFLT
IINAFSSLFNARSIIDGVSLSDKNSIGDMISISALNIYDDGLNDKNISSAPFDGEGTPTKKLSIIKKGKLENFLHSESTA
KIFNTIPTGHAGLGSKVSVSPDWLVIERSSDCSELNMSLEHTNYEGEFVYIEELNAIHAGVRASQGSFSLPFEGWIYSNG
KKSSIESATVAGDIKYLLRNIVNIENEEELTTSGISPHIWISELSITGDE

Sequences:

>Translated_450_residues
MNATEITNQIKHTANILNIDKWDYGASFSNDYSVQVDRGAAKQLKASQKQVITLRVWNKENLVGITTTSDLSETGIKKAL
KQANIASEFGNKNEFTDFSPLAKDPIQVKEIDKKNPLGIKKLLKILREAESKLIDSHEAIKSIPYNGLSESFYERIYANS
DGAFRNYSKSQAALYLYARAEEKDKKPRSSGSVKLGYGVEDIDIESCIKEASKKTIAHLDYSSIKTGKYLVCLSPEAFLT
IINAFSSLFNARSIIDGVSLSDKNSIGDMISISALNIYDDGLNDKNISSAPFDGEGTPTKKLSIIKKGKLENFLHSESTA
KIFNTIPTGHAGLGSKVSVSPDWLVIERSSDCSELNMSLEHTNYEGEFVYIEELNAIHAGVRASQGSFSLPFEGWIYSNG
KKSSIESATVAGDIKYLLRNIVNIENEEELTTSGISPHIWISELSITGDE
>Mature_450_residues
MNATEITNQIKHTANILNIDKWDYGASFSNDYSVQVDRGAAKQLKASQKQVITLRVWNKENLVGITTTSDLSETGIKKAL
KQANIASEFGNKNEFTDFSPLAKDPIQVKEIDKKNPLGIKKLLKILREAESKLIDSHEAIKSIPYNGLSESFYERIYANS
DGAFRNYSKSQAALYLYARAEEKDKKPRSSGSVKLGYGVEDIDIESCIKEASKKTIAHLDYSSIKTGKYLVCLSPEAFLT
IINAFSSLFNARSIIDGVSLSDKNSIGDMISISALNIYDDGLNDKNISSAPFDGEGTPTKKLSIIKKGKLENFLHSESTA
KIFNTIPTGHAGLGSKVSVSPDWLVIERSSDCSELNMSLEHTNYEGEFVYIEELNAIHAGVRASQGSFSLPFEGWIYSNG
KKSSIESATVAGDIKYLLRNIVNIENEEELTTSGISPHIWISELSITGDE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=438, Percent_Identity=21.4611872146119, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49585; Mature: 49585

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATEITNQIKHTANILNIDKWDYGASFSNDYSVQVDRGAAKQLKASQKQVITLRVWNKE
CCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDLSETGIKKALKQANIASEFGNKNEFTDFSPLAKDPIQVKEIDKKNPLGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
KLLKILREAESKLIDSHEAIKSIPYNGLSESFYERIYANSDGAFRNYSKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC
EEKDKKPRSSGSVKLGYGVEDIDIESCIKEASKKTIAHLDYSSIKTGKYLVCLSPEAFLT
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCHHHHH
IINAFSSLFNARSIIDGVSLSDKNSIGDMISISALNIYDDGLNDKNISSAPFDGEGTPTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCH
KLSIIKKGKLENFLHSESTAKIFNTIPTGHAGLGSKVSVSPDWLVIERSSDCSELNMSLE
HHHEEHHCCHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCHHHHCCEEE
HTNYEGEFVYIEELNAIHAGVRASQGSFSLPFEGWIYSNGKKSSIESATVAGDIKYLLRN
ECCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
IVNIENEEELTTSGISPHIWISELSITGDE
HHCCCCCHHHHHCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MNATEITNQIKHTANILNIDKWDYGASFSNDYSVQVDRGAAKQLKASQKQVITLRVWNKE
CCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDLSETGIKKALKQANIASEFGNKNEFTDFSPLAKDPIQVKEIDKKNPLGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
KLLKILREAESKLIDSHEAIKSIPYNGLSESFYERIYANSDGAFRNYSKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEC
EEKDKKPRSSGSVKLGYGVEDIDIESCIKEASKKTIAHLDYSSIKTGKYLVCLSPEAFLT
CCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCHHHHH
IINAFSSLFNARSIIDGVSLSDKNSIGDMISISALNIYDDGLNDKNISSAPFDGEGTPTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCH
KLSIIKKGKLENFLHSESTAKIFNTIPTGHAGLGSKVSVSPDWLVIERSSDCSELNMSLE
HHHEEHHCCHHHHHHCCCHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCHHHHCCEEE
HTNYEGEFVYIEELNAIHAGVRASQGSFSLPFEGWIYSNGKKSSIESATVAGDIKYLLRN
ECCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
IVNIENEEELTTSGISPHIWISELSITGDE
HHCCCCCHHHHHCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]